{"product_id":"biomiga-sybr-green-qpcr-master-mix-no-rox-qp1311","title":"Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e2X\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eReady-to-use mix\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003eHot start\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eAntibody-inactivated Taq\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e3 mM\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eFinal Mg²⁺ at 1X\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003eNo ROX\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eReference dye\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eSYBR GREEN qPCR · NO ROX\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eAdd primers and template. Everything else is in the mix.\u003c\/h2\u003e\n\u003cp\u003eBiomiga's SB-Green 2X qPCR Master Mix (QP1311) is a ready-to-use SYBR Green I master mix for real-time quantitative PCR. It contains hot-start Taq DNA polymerase, dNTPs, Mg²⁺, SYBR Green I dye, enhancer and stabilizers. It contains no internal reference dye, for real-time instruments that do not require ROX. The hot-start Taq is antibody-inactivated, which blocks polymerase activity at room temperature; activity is restored once the reaction reaches 95 °C.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/QP131x-inline-workflow-mobile.png?v=1791229700\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/QP131x-inline-workflow-desktop.png?v=1791229700\" alt=\"SYBR Green qPCR workflow with standard and fast cycling conditions for Biomiga 2X qPCR master mixes\" width=\"1720\" height=\"1226\" loading=\"lazy\" style=\"display:block;width:100%;max-width:860px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:860px;text-align:center\"\u003eBiomiga's suggested starting protocol. Optimal conditions vary with primer set.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eThaw and mix\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eThaw at room temperature, mix gently, spin down and keep on ice.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAssemble\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eCombine the 2X mix, primers, template and water on ice or at room temperature, then seal with optical caps or film and spin down.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eActivate and cycle\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003e95 °C for 2 min, then 30–45 cycles of 95 °C and 60 °C, collecting data at 60 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eMelt curve\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eBiomiga recommends a melt curve on every run to check for primer dimers and confirm specificity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eReaction setup\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:460px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e50 µL reaction\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e20 µL reaction\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eFinal\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e2X qPCR Mix (No ROX)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e25 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eForward primer (10 µM)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.4 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e200 nM (100–500 nM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eReverse primer (10 µM)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.4 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e200 nM (100–500 nM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eTemplate\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003evariable\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003evariable\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 50 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 20 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eReactions scale from 5 to 50 µL; keep all volumes proportional. Adding template in 5–10 µL improves precision, and undiluted cDNA should be no more than 10% of the reaction.\u003c\/p\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eCycling conditions\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:420px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eStep\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eStandard cycling\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eFast cycling\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eTaq activation\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e95 °C, 2 min\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e95 °C, 2 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eDenature (30–45 cycles)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e95 °C, 10–15 s\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e95 °C, 3–5 s\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eAnneal\/extend + data collection\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e60 °C, 30–60 s\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e60 °C, 20–30 s\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eMelt curve\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003ePer instrument\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003ePer instrument\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px;margin:16px 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eTemplate input\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003ecDNA from 1 pg–100 ng of total RNA; genomic DNA 100 pg–100 ng; plasmid DNA 10–10⁷ copies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003ePrimers\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eDesign for 70–150 bp amplicons. 200 nM per primer suits most reactions; titrate between 100 and 500 nM if needed.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eMagnesium\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003e3 mM final works for most targets; the optimum can be 3–6 mM. Add 1, 2 or 3 µL of 50 mM MgCl₂ per 50 µL to reach 4, 5 or 6 mM (MgCl₂ not included).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich ROX version fits my instrument?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/QP1311-inline-rox-mobile.png?v=1791229701\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/QP1311-inline-rox-desktop.png?v=1791229700\" alt=\"Which Biomiga SYBR Green qPCR mix fits your instrument: QP1311 No ROX highlighted\" width=\"1720\" height=\"1024\" loading=\"lazy\" style=\"display:block;width:100%;max-width:860px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:860px;text-align:center\"\u003eSame SYBR Green chemistry; only the ROX reference dye differs. Compatibility per Biomiga.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:460px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eMix\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eROX\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eCompatible instruments (per Biomiga)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e\u003cstrong\u003eQP1311 (this mix)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eNo ROX\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eBio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e\u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-low-rox-qp1312\" style=\"color:#C2570C\"\u003eQP1312\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eLow ROX\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eApplied Biosystems 7500, 7500 Fast and ViiA 7; Stratagene Mx3000P, Mx3005P and Mx4000; Agilent AriaMx\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e\u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-rox-qp1313\" style=\"color:#C2570C\"\u003eQP1313\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eROX\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eApplied Biosystems 7000, 7300, 7700, 7900 and 7900HT; StepOne and StepOnePlus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 16px\"\u003eNot sure? Check your instrument's passive reference (ROX) setting, or email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e.\u003c\/p\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eLOW ROX\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-low-rox-qp1312\" style=\"color:#13202E\"\u003eBiomiga SYBR Green qPCR Mix, Low ROX (QP1312)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eFor Applied Biosystems 7500, 7500 Fast and ViiA 7 and more.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eROX\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-rox-qp1313\" style=\"color:#13202E\"\u003eBiomiga SYBR Green qPCR Mix, ROX (QP1313)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eFor Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT and more.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003ePrimers, template and nuclease-free water; optical plates or tubes and sealing film; optional 50 mM MgCl₂. From our store: \u003ca href=\"\/products\/mtc-bio-96-well-low-profile-pcr-plates-0-1ml\" style=\"color:#C2570C\"\u003e96-well qPCR plates\u003c\/a\u003e, \u003ca href=\"\/products\/mtc-bio-pureamp-qpcr-tubes-strips\" style=\"color:#C2570C\"\u003eqPCR tubes and 8-strips\u003c\/a\u003e and \u003ca href=\"\/products\/mtc-bio-pcr-qpcr-sealing-films-foils\" style=\"color:#C2570C\"\u003eoptical sealing films\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eIn the kit\u003c\/h3\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:260px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003ePack\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e2X mix\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e100 reactions\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e500 reactions\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eEach 2X mix contains hot-start Taq, dNTPs, 6 mM Mg²⁺ (3 mM at 1X), SYBR Green I, enhancer and stabilizers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eBuilt for\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eGene expression\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTwo-step RT-qPCR after cDNA synthesis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eDNA quantification\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eGenomic and plasmid targets on a standard curve.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eCore and teaching labs\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eOne ready-to-use mix across users and plates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this mix is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eYour instrument matches:\u003c\/strong\u003e Bio-Rad CFX96, CFX384 and CFX Connect and the other systems listed above.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eAll-in-one:\u003c\/strong\u003e add only primers, template and water.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eFast cycling:\u003c\/strong\u003e Biomiga supplies a fast protocol with 3–5 s denaturation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eForgiving storage:\u003c\/strong\u003e Biomiga reports no significant performance loss after 10 freeze-thaw cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eRelated Biomiga products\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311\" style=\"color:#13202E\"\u003eCell \u0026amp; Tissue RNA Mini Kit (R6311)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTotal RNA from cells and tissue for RT-qPCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#13202E\"\u003eCell\/Tissue RNA Kit Plus (R6321)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eAdds a gDNA clearance column before qPCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-express-hi-fi-dna-polymerase-ehf1101\" style=\"color:#13202E\"\u003eExpress Hi Fi DNA Polymerase (EHF1101)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eHigh-fidelity PCR for cloning standards and inserts.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eAlso from Biomiga: 1st cDNA Synthesis Kit (FS01) and Probe-based 2X qPCR Master Mix (QP1314). Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a quote.\u003c\/p\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A 2X ready-to-use SYBR Green I qPCR master mix with hot-start Taq (No ROX).\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eInstruments:\u003c\/strong\u003e Bio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePacks:\u003c\/strong\u003e 100 reactions (2 × 1.25 mL) or 500 reactions (10 × 1.25 mL).\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e −20 °C, protected from light.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhich version do I need?\u003c\/summary\u003e\u003cp\u003eMatch the mix to your instrument's ROX requirement. QP1311 (No ROX) is for Bio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex. If your instrument isn't listed, check its passive reference setting or ask us.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan I use this mix with TaqMan or other probes?\u003c\/summary\u003e\u003cp\u003eNo. These mixes use SYBR Green I dye-based detection. For probe assays, ask us about Biomiga's Probe-based 2X qPCR Master Mix (QP1314).\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat do I need to add?\u003c\/summary\u003e\u003cp\u003ePrimers, template and nuclease-free water. Add 50 mM MgCl₂ only if you want more than the built-in 3 mM final.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDoes it support fast cycling?\u003c\/summary\u003e\u003cp\u003eYes. After the 2-minute activation at 95 °C, Biomiga's fast protocol uses 95 °C for 3–5 s and 60 °C for 20–30 s per cycle.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should it be stored?\u003c\/summary\u003e\u003cp\u003eAt −20 °C, protected from light. For convenience it can be kept at 4 °C for up to 6 months. Avoid repeated freeze-thaw, although Biomiga reports no significant performance loss after 10 cycles.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat amplicon size works best?\u003c\/summary\u003e\u003cp\u003e70–150 bp. Biomiga advises keeping amplicons under 500 bp and running a melt curve on every plate to check for primer dimers.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow is the mix qualified?\u003c\/summary\u003e\u003cp\u003eBiomiga functionally tests the mix in qPCR and reports linear resolution over six orders of magnitude (R² \u0026gt; 0.995) with efficiency above 90%.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow many reactions are in a pack?\u003c\/summary\u003e\u003cp\u003e100 reactions (2 × 1.25 mL) or 500 reactions (10 × 1.25 mL) of 2X mix, sized for 50 µL reactions. Reactions can be scaled down to 5 µL, which stretches each pack further.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan I switch from another vendor's SYBR Green mix?\u003c\/summary\u003e\u003cp\u003eUsually, as long as the ROX level matches your instrument. Biomiga has not published side-by-side data, so confirm primer efficiency on a standard curve before switching an established assay.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\n\u003cp style=\"font-size:12px;color:#7A8896;margin:16px 0 0\"\u003eSYBR is a trademark of Thermo Fisher Scientific. Instrument names are trademarks of their respective owners. Blue Tiger Scientific is not affiliated with these companies.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"100 reactions","offer_id":52851423281450,"sku":"BTS-QP1311-01","price":110.0,"currency_code":"USD","in_stock":true},{"title":"500 reactions","offer_id":52851423314218,"sku":"BTS-QP1311-02","price":460.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/QP1311-1.jpg?v=1791229949","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-sybr-green-qpcr-master-mix-no-rox-qp1311","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}