{"product_id":"biomiga-one-step-rt-pcr-kit-rt0201","title":"Biomiga One-Step RT-PCR Kit, MMLV RT + Hot-Start Taq (RT0201)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003eOne tube\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eRT and PCR together\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003eHot start\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eTaq + MMLV RT mix\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003eUp to 4 kb\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eRNA targets (Biomiga)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e0.01 pg–2 µg\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eTotal RNA input\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eONE-STEP RT-PCR\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrom RNA to PCR product in one tube and one program.\u003c\/h2\u003e\n\u003cp\u003eBiomiga's One-Step RT-PCR Kit (RT0201) reverse-transcribes and amplifies a specific RNA target from total RNA or mRNA in a single reaction. It uses a mixture of MMLV reverse transcriptase and hot-start Taq DNA polymerase in an optimized buffer. Biomiga states it can detect RNA targets up to 4.0 kb from 0.01 pg to 2 µg of total RNA. A separate 5 mM magnesium sulfate stock is included for optimization.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/RT0201-inline-workflow-mobile.png?v=1791231026\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/RT0201-inline-workflow-desktop.png?v=1791231026\" alt=\"One-step RT-PCR workflow for Biomiga RT0201: assemble on ice, reverse transcribe at 40–50 °C, amplify for 40 cycles, check the product on a gel\" width=\"1720\" height=\"908\" loading=\"lazy\" style=\"display:block;width:100%;max-width:860px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:860px;text-align:center\"\u003eBiomiga's suggested starting program, 50 µL reactions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAssemble on ice\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003e25 µL 2× reaction mix, 1 µL enzyme mix, forward and reverse primers, and RNA template in a 50 µL reaction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eReverse transcribe\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eProgram the thermal cycler so cDNA synthesis is followed immediately by PCR: 40–50 °C for 10 min.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAmplify\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003e94 °C for 2 min, then 40 cycles of 94 °C for 15 s, 55–65 °C for 30 s and 68 °C for 1 min per kb.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eCheck on a gel\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAnalyze the RT-PCR products by gel electrophoresis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eReaction setup\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:420px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e50 µL reaction\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eFinal\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e2× reaction mix (dNTPs, Mg²⁺)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e25 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eEnzyme mix (MMLV RT \/ hot-start Taq)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eForward primer (10 µM)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e200 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eReverse primer (10 µM)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e200 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eRNA template\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003ex µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.01 pg–2 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 50 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eThermal program\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:380px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eStep\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTemperature\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTime\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eCycles\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ecDNA synthesis\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e40–50 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 min\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eDenaturation\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e94 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2 min\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePCR: denature\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e94 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e15 s\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePCR: anneal\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e55–65 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30 s\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePCR: extend\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e68 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 min per kb\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px;margin:16px 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eMagnesium\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eThe 2× reaction mix already contains Mg²⁺. If needed, optimize further with the supplied 5 mM magnesium sulfate. Note this is MgSO₄, not MgCl₂.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eDetection\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eThe kit produces an end-point PCR product for gel analysis. It contains no fluorescent detection dye, so it is not a real-time qPCR kit.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich kit do I need?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/RT0201-inline-chooser-mobile.png?v=1791231026\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/RT0201-inline-chooser-desktop.png?v=1791231026\" alt=\"Two-step or one-step: Biomiga RT0201 One-Step RT-PCR Kit highlighted, compared with the FS01 1st Strand cDNA Synthesis Kit\" width=\"1720\" height=\"1038\" loading=\"lazy\" style=\"display:block;width:100%;max-width:860px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:860px;text-align:center\"\u003eBoth kits start from purified RNA; they differ in how the cDNA is made and used.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 220px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTHIS KIT\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0 6px;font-size:17px\"\u003eOne-Step RT-PCR Kit (RT0201)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eRT and PCR in one tube with your own primers; end-point product for gel analysis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTWO-STEP\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-1st-strand-cdna-synthesis-kit-fs01\" style=\"color:#13202E\"\u003e1st Strand cDNA Synthesis Kit (FS01)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eMakes a reusable cDNA pool for qPCR or several PCR assays.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eNeed quantitative results? Make cDNA with \u003ca href=\"\/products\/biomiga-1st-strand-cdna-synthesis-kit-fs01\" style=\"color:#C2570C\"\u003eFS01\u003c\/a\u003e, then run a \u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-no-rox-qp1311\" style=\"color:#C2570C\"\u003eBiomiga SYBR Green qPCR mix\u003c\/a\u003e matched to your instrument.\u003c\/p\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003eForward and reverse primers, purified RNA (for example from the \u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311\" style=\"color:#C2570C\"\u003eR6311\u003c\/a\u003e or \u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#C2570C\"\u003eR6321\u003c\/a\u003e kits), nuclease-free water and gel electrophoresis supplies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eIn the box\u003c\/h3\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:300px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e100 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eEnzyme mix (MMLV RT \/ hot-start Taq)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e2× reaction mix (contains dNTPs and Mg²⁺)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e5 mM magnesium sulfate\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eThe 5- and 25-reaction packs contain the same three components in 5- and 25-reaction quantities.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this kit is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eOne target, fast answer:\u003c\/strong\u003e RNA in, PCR product out in a single program\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eFewer handling steps:\u003c\/strong\u003e no separate cDNA step or tube transfer\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eHot start:\u003c\/strong\u003e hot-start Taq in the enzyme mix for the PCR stage\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eTrial size:\u003c\/strong\u003e the 5-reaction pack lets you test your primers first\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eRelated Biomiga products\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-1st-strand-cdna-synthesis-kit-fs01\" style=\"color:#13202E\"\u003e1st Strand cDNA Synthesis Kit (FS01)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTwo-step route for qPCR and multiple assays.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311\" style=\"color:#13202E\"\u003eCell \u0026amp; Tissue RNA Mini Kit (R6311)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTotal RNA from cells and tissue.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#13202E\"\u003eCell\/Tissue RNA Kit Plus (R6321)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eAdds a gDNA clearance column.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-express-hi-fi-dna-polymerase-ehf1101\" style=\"color:#13202E\"\u003eExpress Hi Fi DNA Polymerase (EHF1101)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eHigh-fidelity PCR when you need to clone.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A one-step RT-PCR kit with MMLV RT and hot-start Taq in one enzyme mix\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eTargets:\u003c\/strong\u003e Up to 4.0 kb, per Biomiga\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eRNA input:\u003c\/strong\u003e 0.01 pg–2 µg total RNA per 50 µL reaction\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePacks:\u003c\/strong\u003e 5, 25 or 100 reactions\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e −20 °C; stable 2 years\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat is included?\u003c\/summary\u003e\u003cp\u003eAn enzyme mix (MMLV RT and hot-start Taq), a 2× reaction mix containing dNTPs and Mg²⁺, and 5 mM magnesium sulfate. You supply primers, RNA and water.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan I use it for real-time qPCR?\u003c\/summary\u003e\u003cp\u003eNo. Biomiga's protocol is for end-point detection by gel, and the kit contains no fluorescent dye. For quantitative work, make cDNA with \u003ca href=\"\/products\/biomiga-1st-strand-cdna-synthesis-kit-fs01\" style=\"color:#C2570C\"\u003eFS01\u003c\/a\u003e and use a SYBR Green qPCR mix, or ask us about Biomiga's one-step qRT-PCR mix.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat RT temperature should I use?\u003c\/summary\u003e\u003cp\u003eBiomiga suggests 40–50 °C for 10 minutes, followed immediately by PCR.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow long should the extension be?\u003c\/summary\u003e\u003cp\u003e68 °C for 1 minute per kb of target, for 40 cycles.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhy magnesium sulfate?\u003c\/summary\u003e\u003cp\u003eThe 2× reaction mix already contains magnesium. The 5 mM MgSO₄ is supplied for further optimization if needed; use it rather than MgCl₂.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eShould I choose this kit or the 1st Strand cDNA Synthesis Kit?\u003c\/summary\u003e\u003cp\u003eChoose RT0201 for a quick end-point answer on a specific target. Choose FS01 when you want a cDNA pool for qPCR or several assays.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should the kit be stored?\u003c\/summary\u003e\u003cp\u003eAt −20 °C. Biomiga states the kit is stable for 2 years when stored this way.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\u003cp style=\"font-size:12px;color:#7A8896;margin:16px 0 0\"\u003eInstrument and product names are trademarks of their respective owners.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"5 reactions","offer_id":52851506086186,"sku":"BTS-RT0201-00","price":40.0,"currency_code":"USD","in_stock":true},{"title":"25 reactions","offer_id":52851506118954,"sku":"BTS-RT0201-01","price":150.0,"currency_code":"USD","in_stock":true},{"title":"100 reactions","offer_id":52851506151722,"sku":"BTS-RT0201-02","price":415.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/RT0201-1.jpg?v=1791231372","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-one-step-rt-pcr-kit-rt0201","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}