{"product_id":"biomiga-lentivirus-concentration-reagent-v2001","title":"Biomiga Lentivirus Concentration Reagent, No Ultracentrifuge (V2001)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e50–100×\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eBiomiga-stated concentration\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e~60–70%\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eBiomiga-stated recovery\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e30 mL\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eSupernatant per prep\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e15–25 °C\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eBuffer storage\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eLENTIVIRUS CONCENTRATION\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eConcentrate lentivirus on a benchtop centrifuge, not an ultracentrifuge.\u003c\/h2\u003e\n\u003cp\u003eUltracentrifugation has been the traditional way to separate lentivirus from cellular proteins and media, but Biomiga notes it is time-consuming and limits how much material you can process. The Biomiga Lentivirus Concentration Reagent (V2001) concentrates recombinant lentivirus from transfected-cell culture supernatant with two buffers and low-speed spins. Biomiga reports the virus can be concentrated 50–100-fold, with a recovery rate of around 60–70%. Biomiga states the virus is stable in Buffer LP, so the incubation can run overnight.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in humans. All steps require at least Biosafety Level 2 conditions.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/V2001-inline-workflow-mobile.png?v=1791157436\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/V2001-inline-workflow-desktop.png?v=1791157436\" alt=\"Lentivirus concentration workflow with Biomiga V2001: clarify, add Buffer LP, pellet at 3,000 rpm, resuspend in Buffer LS, store at −80 °C\" width=\"1640\" height=\"974\" loading=\"lazy\" style=\"display:block;width:100%;max-width:820px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:820px;text-align:center\"\u003eConditions from Biomiga's instructions for use; up to 30 mL of supernatant per prep.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eClarify\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eSpin the culture media 10 min at 3,000 rpm, 4 °C, and pass the supernatant through a 0.45 µm filter. Clarified supernatant can be stored at −80 °C until you're ready.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAdd Buffer LP\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eMix 1 volume of Buffer LP with 3 volumes of supernatant (for example, 5 mL into 15 mL). Incubate at 4 °C for at least 4 hours or overnight.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003ePellet\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eSpin 30 min at 3,000 rpm, 4 °C. Remove all the supernatant; the pellet may look hazy. Keep it on ice.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eResuspend and store\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eDissolve the pellet in 300–500 µL Buffer LS, spin 2 min at 8,000 rpm, and transfer the supernatant to a clean vial. Aliquot and store at −80 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eProtocol at a glance\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:440px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eStep\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eSpeed \/ temperature\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTime\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eClarify\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e3,000 rpm, 4 °C, then 0.45 µm filter\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eBuffer LP incubation\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e4 h to overnight\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePellet\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e3,000 rpm, 4 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eResuspend and clear\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e300–500 µL Buffer LS; 8,000 rpm, 4 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eStore\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e−80 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich pack do I need?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/V2001-inline-packs-mobile.png?v=1791157436\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/V2001-inline-packs-desktop.png?v=1791157436\" alt=\"Which V2001 pack to choose: supernatant capacity for each pack size, three times the Buffer LP volume\" width=\"1640\" height=\"1418\" loading=\"lazy\" style=\"display:block;width:100%;max-width:820px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:820px;text-align:center\"\u003eSupernatant capacity is 3 × the Buffer LP volume, from Biomiga's 1 : 3 ratio.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:440px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003ePack\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eBuffer LP\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eBuffer LS\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eSupernatant it can treat\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eV2001-00\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eup to 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eV2001-01\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e100 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eup to 300 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eV2001-02\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e500 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eup to 1.5 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eV2001-03\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2,000 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eincluded\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eup to 6 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px;margin:16px 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTRIAL SIZE\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003eV2001-00 · 10 mL\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eOne full 30 mL prep, or a first trial on your own supernatant.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eV2001-01 · 100 mL\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eRegular small-scale production.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eV2001-02 · 500 mL\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eLarge or pooled harvests.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eV2001-03 · 2,000 mL\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eCore facilities and scale-up.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003eClarified virus supernatant, 0.45 µm filters, sterile conical and microcentrifuge tubes, a refrigerated centrifuge (3,000 rpm), a microcentrifuge (8,000 rpm, 4 °C), cryovials and a BSL-2 workspace.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eIn the kit\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eBuffer LP\u003c\/strong\u003e: added at 1 volume per 3 volumes of supernatant.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eBuffer LS\u003c\/strong\u003e: 300–500 µL per prep to resuspend the virus pellet.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eVolumes for each pack are in the table above.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eBuilt for\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eTransduction stocks\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eRaise titer before infecting hard-to-transduce cells.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eLabs without an ultracentrifuge\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eA refrigerated benchtop centrifuge is enough.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eViral vector cores\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eScale up with the 500 mL and 2,000 mL packs.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this reagent is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eNo ultracentrifuge access:\u003c\/strong\u003e low-speed spins at 3,000 rpm.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eOvernight-friendly:\u003c\/strong\u003e Biomiga states the virus is stable in Buffer LP, so the incubation can run overnight.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eSimple storage:\u003c\/strong\u003e both buffers store at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eTry before scaling:\u003c\/strong\u003e the 10 mL pack treats one full 30 mL prep.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eRelated Biomiga kits\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-endofree-ezfilter-plasmid-maxiprep-kit\" style=\"color:#13202E\"\u003eEndoFree ezFilter Plasmid Maxiprep Kit (PD1522)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eEndotoxin-free plasmid prep for the transfection step upstream.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eLentivirus Purification Mini and Maxi Kits (V1170, V1270)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eBiomiga's lentivirus purification kits. Ask us for details.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eNeed one of the others? Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a quote.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A two-buffer reagent set for concentrating recombinant lentivirus from culture supernatant without ultracentrifugation.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eRatio:\u003c\/strong\u003e 1 volume Buffer LP to 3 volumes supernatant.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePer prep:\u003c\/strong\u003e up to 30 mL supernatant; 300–500 µL Buffer LS.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePack sizes:\u003c\/strong\u003e 10, 100, 500 and 2,000 mL.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only, under BSL-2 conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow much does it concentrate the virus?\u003c\/summary\u003e\u003cp\u003eBiomiga reports 50–100-fold concentration with a recovery rate of around 60–70%. Results depend on your construct, packaging system and harvest.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDo I need an ultracentrifuge?\u003c\/summary\u003e\u003cp\u003eNo. Biomiga's protocol uses a refrigerated centrifuge at 3,000 rpm and a microcentrifuge at 8,000 rpm, both at 4 °C. Biomiga gives speeds in rpm, so check your rotor's g-force.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow much supernatant can I process?\u003c\/summary\u003e\u003cp\u003eUp to 30 mL per prep, about 1–2 T75 flasks. Each prep takes one third of its volume in Buffer LP, so 30 mL of supernatant needs 10 mL. Across a whole pack, the supernatant you can treat is three times the Buffer LP volume.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow long does it take?\u003c\/summary\u003e\u003cp\u003ePlan for an incubation of at least 4 hours, or overnight, at 4 °C, plus about 45 minutes of spins. Clarified supernatant can be stored at −80 °C before you start.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should the reagent and the virus be stored?\u003c\/summary\u003e\u003cp\u003eStore both buffers at room temperature (15–25 °C). Aliquot the concentrated virus and store it at −80 °C. The expiry date is on the label.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat biosafety level is required?\u003c\/summary\u003e\u003cp\u003eBiomiga warns that lentivirus-containing media and concentrated virus can be infectious to humans and animals. All steps must be performed under at least Biosafety Level 2 conditions.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow does it compare with Takara's Lenti-X Concentrator?\u003c\/summary\u003e\u003cp\u003eBoth use 1 volume of reagent per 3 volumes of clarified, 0.45 µm-filtered supernatant, followed by a low-speed spin at 4 °C instead of ultracentrifugation. Takara's published protocol calls for 30 minutes to overnight at 4 °C and a 1,500 × g spin for 45 minutes. Biomiga specifies at least 4 hours to overnight and 30 minutes at 3,000 rpm. We don't compare recovery between the two products; validate on your own supernatant, and the 10 mL pack is sized for a trial. Lenti-X is a trademark of Takara Bio Inc.; Blue Tiger Scientific is not affiliated with Takara Bio.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan it be used for clinical or in-human work?\u003c\/summary\u003e\u003cp\u003eNo. It is for in vitro research use only and not for use in humans.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"10 mL","offer_id":52839762362666,"sku":"BTS-V2001-00","price":24.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":52839762395434,"sku":"BTS-V2001-01","price":154.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":52839762428202,"sku":"BTS-V2001-02","price":508.0,"currency_code":"USD","in_stock":true},{"title":"2,000 mL","offer_id":52839762460970,"sku":"BTS-V2001-03","price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/V2001-1.jpg?v=1791157644","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-lentivirus-concentration-reagent-v2001","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}