{"product_id":"biomiga-express-hi-fi-dna-polymerase-ehf1101","title":"Biomiga Express Hi Fi DNA Polymerase, High-Fidelity PCR Enzyme (EHF1101)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e2 U\/µL\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eEnzyme concentration\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e10 s\/kb\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eExtension at 72 °C (routine protocol)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e500 \/ 2,500 U\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003ePack sizes\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003eBlunt\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003ePCR product ends\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBIOMIGA PCR ENZYMES\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eA high-fidelity PCR enzyme with everything you need to tune it.\u003c\/h2\u003e\n\u003cp\u003eExpress Hi Fi DNA Polymerase from Biomiga ships with its own 5X reaction buffer, a separate 20 mM MgCl₂ stock and DMSO, so you can run Biomiga's routine protocol as written or adjust magnesium and additives for a difficult template. The 1X buffer delivers 1.5 mM Mg²⁺; Biomiga recommends optimizing in 0.2 mM steps using the supplied MgCl₂. Extension runs at 72 °C for 10 seconds per kb, and the PCR products have blunt ends.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/EHF1101-inline-workflow-mobile.png?v=1791154355\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/EHF1101-inline-workflow-desktop.png?v=1791154355\" alt=\"Routine PCR workflow and thermal profile for Biomiga Express Hi Fi DNA Polymerase: 95 °C 30 s, 25–35 cycles of 95 °C, 52–68 °C and 72 °C at 10 s per kb, 72 °C 8 min final extension\" width=\"1600\" height=\"1628\" loading=\"lazy\" style=\"display:block;width:100%;max-width:800px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:800px;text-align:center\"\u003eBiomiga's routine PCR outline. Optimize annealing temperature and cycle number for your template.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAssemble on ice\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eCombine 5X buffer, dNTPs, primers, template and optional DMSO; add the enzyme last and mix gently.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eStart hot\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eMove tubes straight from ice into a thermocycler already at denaturation temperature.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eCycle\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003e95 °C for 30 s, then 25–35 cycles of 95 °C, 52–68 °C and 72 °C at 10 s per kb; finish at 72 °C for 8 min.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eClone blunt\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eBiomiga notes the products have blunt ends and recommends blunt-end cloning.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eReaction setup\u003c\/h2\u003e\n\u003cp\u003eFrom Biomiga's instructions for use. Assemble on ice.\u003c\/p\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:480px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e20 µL reaction\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e50 µL reaction\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eFinal\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e5X Express Hi Fi Buffer\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e4 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1X (1.5 mM Mg²⁺)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mM dNTPs\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.4 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e200 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e10 µM forward primer\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2.5 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.5 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003e10 µM reverse primer\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2.5 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.5 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eTemplate DNA\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003evariable\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003evariable\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eunder 250 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eDMSO (optional)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eper IFU\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eper IFU\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eExpress Hi Fi DNA Polymerase\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e0.5 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 20 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 50 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eThermocycling (routine PCR)\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:420px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eStep\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTemperature\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTime\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eInitial denaturation\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e95 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30 s\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\" rowspan=\"3\"\u003e25–35 cycles\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e95 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e5–10 s\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:10px\"\u003e52–68 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10–30 s\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:10px\"\u003e72 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 s per kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eFinal extension\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e72 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e8 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eHold\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e4–8 °C\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px;margin:16px 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eTemplate amount (50 µL)\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eGenomic DNA 50–250 ng; plasmid or viral DNA 10–50 ng. High-quality template greatly improves results.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003ePrimers and Mg²⁺\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003ePrimers can run at 0.2–1 µM final. Mg²⁺ is affected by dNTPs, template, additives and EDTA; adjust in 0.2 mM steps with the supplied MgCl₂.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003edNTPs, forward and reverse primers, template DNA, nuclease-free water, PCR tubes and a thermocycler (with a heated lid, or mineral oil to overlay).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eWhat's in the box\u003c\/h3\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto\"\u003e\u003ctable style=\"width:100%;min-width:300px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:8px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003eEHF1101-01\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003eEHF1101-02\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eExpress Hi Fi DNA Polymerase (2 U\/µL)\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e500 U (250 µL)\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e2,500 U (1,250 µL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003e5X Buffer\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e2 × 1,200 µL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e10 × 1,200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003e20 mM MgCl₂\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eDMSO\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e500 µL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e1,000 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eBuilt for\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eCloning labs\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eAmplifying inserts for blunt-end vectors.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eConstruct work\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eMg²⁺ and DMSO as separate knobs for difficult or GC-rich templates.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eCore and teaching labs\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eOne enzyme standardized across many users with the 2,500 U pack.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich format do I need?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/EHF1101-inline-format-mobile.png?v=1791154355\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/EHF1101-inline-format-desktop.png?v=1791154355\" alt=\"Comparison of Biomiga EHF1101 separate enzyme and buffer versus EHF1102 2X master mix, with EHF1101 pack sizes\" width=\"1600\" height=\"1196\" loading=\"lazy\" style=\"display:block;width:100%;max-width:800px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:800px;text-align:center\"\u003eSeparate enzyme for tuning, or Biomiga's premixed 2X master mix for fewer pipetting steps.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 240px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTHIS KIT\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003eExpress Hi Fi DNA Polymerase (EHF1101)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eEnzyme, 5X buffer, MgCl₂ and DMSO supplied separately. You add dNTPs. Best when you want control over Mg²⁺, DMSO and enzyme amount.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eExpress Hi Fi 2X Master Mix (EHF1102)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eBiomiga premixes the same polymerase with buffer, Mg²⁺ and dNTPs; you add primers and template. 100- or 500-reaction packs.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch;margin:16px 0 0\"\u003e\u003ctable style=\"width:100%;min-width:440px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003e\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eEHF1101 (this kit)\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eEHF1102 Master Mix\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eSeparate enzyme + 5X buffer\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e2X ready mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eMg²⁺ \/ DMSO adjustment\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eSupplied separately\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eMg²⁺ pre-set in mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003edNTPs\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eYou add\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eIncluded\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eYou add\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003edNTPs, primers, template, water\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003ePrimers, template, water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePacks\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e500 U, 2,500 U\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e100, 500 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eNeed the master mix? Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a quote.\u003c\/p\u003e\n\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this kit is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eBlunt-end cloning:\u003c\/strong\u003e Biomiga's products have blunt ends, ready for blunt-end vectors.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eFast extension:\u003c\/strong\u003e 10 seconds per kb at 72 °C in the routine protocol.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eSeparate knobs:\u003c\/strong\u003e MgCl₂ and DMSO come as their own tubes rather than a fixed master mix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eTwo scales:\u003c\/strong\u003e 500 U to get started, 2,500 U with buffer and additives scaled to match.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eRelated Biomiga kits\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-endofree-ezfilter-plasmid-maxiprep-kit\" style=\"color:#13202E\"\u003eEndoFree ezFilter Plasmid Maxiprep Kit (PD1522)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003ePurify transfection-grade plasmid after cloning your blunt-end insert.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A high-fidelity PCR DNA polymerase supplied with its own buffer, MgCl₂ and DMSO.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 2 U\/µL.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eProducts:\u003c\/strong\u003e Blunt-ended.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePack sizes:\u003c\/strong\u003e EHF1101-01 (500 U) and EHF1101-02 (2,500 U). Ships frozen.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat comes in each pack?\u003c\/summary\u003e\u003cp\u003eEvery pack contains the enzyme at 2 U\/µL, 5X Express Hi Fi Buffer, 20 mM MgCl₂ and DMSO. EHF1101-01 has 500 U (250 µL) of enzyme; EHF1101-02 has 2,500 U (1,250 µL), with buffer, MgCl₂ and DMSO scaled up to match. See the box contents table above.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat kind of ends do the PCR products have?\u003c\/summary\u003e\u003cp\u003eBiomiga states the products have blunt ends and recommends blunt-end cloning. Blunt products lack the single 3′ A overhang that TA-cloning vectors need.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eAbout how many reactions does a pack run?\u003c\/summary\u003e\u003cp\u003eAt the IFU's 1 µL of enzyme per 50 µL reaction, the enzyme volume works out to roughly 250 reactions (EHF1101-01) or 1,250 (EHF1101-02); the buffer at 10 µL per reaction covers about 240 or 1,200. Smaller 20 µL reactions stretch both further. These are approximate divisions of Biomiga's volumes, not a stated reaction count.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDo I need to add magnesium?\u003c\/summary\u003e\u003cp\u003eNot for a standard run: the 1X buffer already gives 1.5 mM Mg²⁺. Biomiga notes that dNTP concentration, the template, additives and any EDTA affect the optimum, and suggests adjusting in 0.2 mM steps with the MgCl₂ provided.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhen should I use the DMSO?\u003c\/summary\u003e\u003cp\u003eFor GC-rich templates or sequences with strong secondary structure. Follow the current instructions for use for the final concentration.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow much template should I use?\u003c\/summary\u003e\u003cp\u003eFor a 50 µL reaction, Biomiga suggests 50–250 ng of genomic DNA or 10–50 ng of plasmid or viral DNA. High-quality template improves results.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan I run it in a protocol written for another vendor's high-fidelity enzyme?\u003c\/summary\u003e\u003cp\u003eUse Biomiga's buffer, magnesium and cycling conditions rather than another product's. Biomiga has not published a side-by-side comparison, so run a small pilot before switching an established assay.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eIs there a ready-to-use version?\u003c\/summary\u003e\u003cp\u003eYes. Biomiga's EHF1102 2X Master Mix premixes the polymerase with buffer, Mg²⁺ and dNTPs. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e to order it.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"500 U","offer_id":52839594623274,"sku":"BTS-EHF1101-01","price":375.0,"currency_code":"USD","in_stock":true},{"title":"2,500 U","offer_id":52839594656042,"sku":"BTS-EHF1101-02","price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/EHF1101-1.jpg?v=1791154605","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-express-hi-fi-dna-polymerase-ehf1101","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}