{"product_id":"biomiga-endofree-ezfilter-plasmid-maxiprep-kit","title":"Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e\u0026lt;0.1 EU\/µg\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eEndotoxin in purified DNA\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e1,200 µg\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eBinding capacity per column\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e150–400 mL\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eE. coli culture per prep\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e~5 min\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eLysate clearing by syringe filter\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eWHY ENDOTOXIN-FREE\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eClean plasmid for the cells that notice.\u003c\/h2\u003e\n\u003cp\u003ePlasmid made with a standard prep carries bacterial endotoxin (lipopolysaccharide) from the \u003cem\u003eE. coli\u003c\/em\u003e outer membrane. Robust lines like HEK293 tolerate it, but endotoxin-sensitive cell lines, primary cells and microinjection work call for it to be removed first. The Biomiga EndoFree ezFilter Plasmid Maxiprep Kit (PD1522) strips endotoxin inside the purification itself and delivers plasmid below 0.1 EU per µg, eluted directly in 1.5 to 2 mL with no precipitation step.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eLyse and clear\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eResuspend, lyse and neutralize, then push the lysate through the 60 mL ezFilter syringe. About five minutes instead of a 20-minute high-speed spin.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eStrip endotoxin and bind\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eEndotoxin-removal buffer and ethanol go into the cleared lysate, which then loads onto the maxi column. No phase-partition extraction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eWash and dry\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eWash the column and dry it, on a vacuum manifold or in a centrifuge.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eElute\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eElute in 1.5 to 2 mL of EndoFree Elution Buffer and reapply once for a second pass.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eFull volumes, speeds and the centrifuge-only protocol are in the user manual.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eExpected yield\u003c\/h2\u003e\n\u003cp\u003eYield depends on the plasmid's origin of replication, its size and the host strain. Biomiga's figures per 200 mL of culture:\u003c\/p\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:440px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003ePlasmid\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eOrigin\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eCopy number\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eYield (µg \/ 200 mL)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epUC\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eMutated pMB1\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e500–700\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e\u003cstrong\u003e700–1,000\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epBluescript\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eColE1\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e300–500\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e450–600\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epGEM\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eMutated pMB1\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e300–400\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e350–450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003epMB1\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e15–20\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e35–50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epSuperCos\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003epMB1\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10–20\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30–50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epACYC\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eP15A\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10–12\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e25–40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003epSC101\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003epSC101\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eGetting the most plasmid\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-left:4px solid #2E7D5B;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px;color:#2E7D5B\"\u003eDo\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003eUse an \u003cem\u003eendA\u003c\/em\u003e- host such as DH5α or TOP10\u003c\/li\u003e\n\u003cli\u003eGrow 12 to 16 hours in LB to an OD600 of 2.0 to 3.0\u003c\/li\u003e\n\u003cli\u003eResuspend the pellet completely before lysis\u003c\/li\u003e\n\u003cli\u003eWarm Buffer B1 to redissolve any precipitate, and keep its cap tight\u003c\/li\u003e\n\u003cli\u003eScale culture and buffers up 2 to 3 times for low-copy plasmids\u003c\/li\u003e\n\u003cli\u003eDry the column fully before eluting\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-left:4px solid #B3261E;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px;color:#B3261E\"\u003eDon't\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003eOverload the column: biomass (OD600 x mL) is optimal at 450 to 550\u003c\/li\u003e\n\u003cli\u003eVortex after adding B1 or lyse longer than 5 minutes\u003c\/li\u003e\n\u003cli\u003eHold culture at 4 °C overnight; freeze the pellet at -20 °C instead\u003c\/li\u003e\n\u003cli\u003eForget to add RNase A to Buffer A1\u003c\/li\u003e\n\u003cli\u003eUse rich media above OD600 3.0\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eWorking with an \u003cem\u003eendA\u003c\/em\u003e+ strain such as HB101 or JM101? Ask us about Biomiga PD1713.\u003c\/p\u003e\n\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eEZFILTER VS. STANDARD MAXI\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhy the ezFilter version?\u003c\/h2\u003e\n\u003cp\u003eClearing the lysate is the slowest, most equipment-hungry step of a maxiprep. The ezFilter syringe turns it into a five-minute bench step, so you don't need a high-speed rotor for clearing or wait out a 20-minute spin. With a vacuum manifold, several preps run side by side.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eYou supply:\u003c\/strong\u003e 100% ethanol, 50 mL conical tubes, and a centrifuge reaching 5,000 x g or a vacuum manifold with a 200 to 600 mbar pump.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eWhat's in the box (25 preps)\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003e25 ezBind maxi columns\u003c\/li\u003e\n\u003cli\u003e25 filter syringes, 60 mL\u003c\/li\u003e\n\u003cli\u003eBuffers A1 and B1, 270 mL each\u003c\/li\u003e\n\u003cli\u003eBuffer N3, 80 mL\u003c\/li\u003e\n\u003cli\u003eBuffer RET, 2 x 250 mL\u003c\/li\u003e\n\u003cli\u003eBuffers ER (13 mL) and D1 (27 mL)\u003c\/li\u003e\n\u003cli\u003eEndoFree Elution Buffer, 135 mL\u003c\/li\u003e\n\u003cli\u003eRNase A, 20 mg\/mL, 1.35 mL\u003c\/li\u003e\n\u003cli\u003eUser manual\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eThe 2-prep trial and 10-prep sizes contain the same components scaled down.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eBuilt for labs where endotoxin matters\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003ePrimary cell work\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTransfect sensitive primary cultures without endotoxin in the prep.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eSensitive cell lines\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eFor lines that respond to endotoxin, where HEK293-grade preps fall short.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eMicroinjection\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eEndotoxin-free plasmid for injection work.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eCore facilities\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eRun several maxipreps in parallel on a vacuum manifold.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich Biomiga plasmid kit do I need?\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTHIS KIT\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003eEndoFree ezFilter Maxi (PD1522)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eEndotoxin-free, 150–400 mL culture, fastest lysate clearing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eEndoFree ezFlow Maxi (PD1520)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eSame endotoxin-free maxi scale, without the syringe filter.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eEndoFree ezFlow Midi (PD1420)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eEndotoxin-free DNA at midi scale for smaller cultures.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003ePlasmid ezFilter Maxi (PD1512)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eWhen endotoxin doesn't matter: cloning, mapping, sequencing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eNeed one of the others? Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a quote.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it compares\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:460px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eFeature\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eBiomiga PD1522\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eQIAGEN EndoFree Plasmid Maxi\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eChemistry\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eezBind DNA binding column\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eAnion-exchange resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eProcessing\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eVacuum or centrifuge\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eGravity flow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eLysate clearing\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e60 mL ezFilter syringe\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eQIAfilter cartridge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eEndotoxin removal\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eIntegrated (Buffer RET)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eIntegrated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eStated endotoxin\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eBelow 0.1 EU\/µg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eBelow 0.04 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eSpecifications as published by each manufacturer. QIAGEN and EndoFree are trademarks of QIAGEN; Blue Tiger Scientific is not affiliated with QIAGEN.\u003c\/p\u003e\n\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this kit is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eBigger cultures:\u003c\/strong\u003e accepts 150 to 400 mL per prep, with headroom for low-copy plasmids.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eYour equipment, your way:\u003c\/strong\u003e vacuum manifold or centrifuge, no gravity-flow waiting.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eReady to use:\u003c\/strong\u003e direct elution in 1.5 to 2 mL, no alcohol precipitation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eTry before you stock up:\u003c\/strong\u003e a 2-prep trial size lets you validate it on your own plasmid first.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"margin:14px 0 0;font-size:14px;color:#C9D5E1\"\u003eIf your application needs the lowest possible endotoxin, compare stated levels above and ask us before ordering.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A maxiprep kit that purifies endotoxin-free plasmid DNA from 150–400 mL of \u003cem\u003eE. coli\u003c\/em\u003e culture.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eBest for:\u003c\/strong\u003e Transfection of endotoxin-sensitive cell lines and primary cells, and microinjection.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eAlternative to:\u003c\/strong\u003e QIAGEN EndoFree Plasmid Maxi and similar endotoxin-free maxi kits.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eOutput:\u003c\/strong\u003e Up to 1,000 µg of a high-copy plasmid from 200 mL, below 0.1 EU\/µg endotoxin.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePack sizes:\u003c\/strong\u003e 2 preps (trial), 10 preps and 25 preps.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eIs this an alternative to the QIAGEN EndoFree Plasmid Maxi Kit?\u003c\/summary\u003e\u003cp\u003eYes, for labs that want endotoxin-free plasmid at maxi scale. Both build endotoxin removal into the prep. The Biomiga kit uses a binding column on a vacuum manifold or centrifuge and elutes directly; the QIAGEN kit uses anion-exchange chromatography under gravity flow. QIAGEN states a lower endotoxin level (below 0.04 EU\/µg versus below 0.1 EU\/µg).\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow much plasmid DNA will I get?\u003c\/summary\u003e\u003cp\u003eBiomiga lists 700 to 1,000 µg from 200 mL of culture for a high-copy pUC plasmid, and less for lower-copy vectors. See the yield table above.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow long does the prep take?\u003c\/summary\u003e\u003cp\u003eBiomiga positions its EndoFree ezFlow line as a prep of under 60 minutes, not counting the overnight culture. Times vary with the protocol and the number of preps.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDo I need a vacuum manifold?\u003c\/summary\u003e\u003cp\u003eNo. The manual includes a centrifuge-only protocol. A manifold makes it easier to run several preps at once.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan I use it for low-copy plasmids?\u003c\/summary\u003e\u003cp\u003eYes. Scale the culture volume and the buffer volumes up 2 to 3 times, as Biomiga recommends.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eIs the DNA suitable for primary cells?\u003c\/summary\u003e\u003cp\u003eBiomiga designed the endotoxin-free DNA for transfection of endotoxin-sensitive cell lines, primary cultured cells and microinjection.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should the kit be stored?\u003c\/summary\u003e\u003cp\u003eAt room temperature (22 to 25 °C), except Buffer A1 after RNase A is added and Buffer ER, which go to 4 °C. Shelf life is guaranteed for 12 months from purchase.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan this kit be used for clinical or in vivo human use?\u003c\/summary\u003e\u003cp\u003eNo. It is intended for in vitro research use only and is not for use in humans.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"2 Preps","offer_id":52823404183850,"sku":"BTS-PD1522-00","price":45.0,"currency_code":"USD","in_stock":true},{"title":"10 Preps","offer_id":52823816077610,"sku":"BTS-PD1522-01","price":165.0,"currency_code":"USD","in_stock":true},{"title":"25 Preps","offer_id":52823404216618,"sku":"BTS-PD1522-02","price":328.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/PD1522-5-2000x1500.jpg?v=1790981258","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-endofree-ezfilter-plasmid-maxiprep-kit","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}