{"product_id":"biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311","title":"Biomiga Cell \u0026 Tissue Total RNA Extraction Kit, Spin Column (R6311)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e~30 min\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eBiomiga-stated prep time\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e50–100 µL\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eElution volume\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e\u0026gt;200 nt\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eRNA retained by the column\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e4 \/ 50 \/ 250\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003ePreps per kit\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eTOTAL RNA, CELLS AND TISSUE\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eTotal RNA from cells and tissue in a single spin-column run.\u003c\/h2\u003e\n\u003cp\u003eThe Biomiga Cell \u0026amp; Tissue Total RNA Extraction Kit (R6311) isolates total RNA from cultured cells and animal tissue without phenol\/chloroform extraction. Samples are lysed in Buffer LY, bound to an RNA column with ethanol, washed, and eluted in 50–100 µL of DEPC-treated water. Biomiga describes the RNA as ready for RT-PCR, real-time PCR, Northern analysis and microarray work, and especially suited to quantitative gene-expression studies.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6311-gallery-2-workflow.png?v=1791150742\" alt=\"R6311 workflow overview: cell or tissue RNA preparation, spin-column purification, purified RNA for RT-PCR, real-time PCR, Northern and microarray\" loading=\"lazy\" style=\"display:block;width:100%;max-width:640px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:640px;text-align:center\"\u003eWorkflow overview; follow the R6311 user manual for volumes, speeds and incubation times.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eLyse\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAdd 500 µL Buffer LY (with β-mercaptoethanol added) to the cell pellet or well. Homogenize tissue in Buffer LY with a rotor-stator or ultrasonic homogenizer.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eBind\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAdd half a volume of 100% ethanol to the lysate, then spin it through the RNA column.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eWash\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eWash with Buffer RB, then RNA Wash Buffer. An optional on-column DNase I step fits in between. Finish with a dry spin, lid open.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eElute\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAdd 50–100 µL of DEPC-treated water and spin. Store the RNA at −20 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eFull protocol, volumes and speeds are in the user manual.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eInput and expected yield\u003c\/h2\u003e\n\u003cp\u003eBiomiga's recommended maximum per prep, with reported total RNA yields. Yields vary with sample quality and handling.\u003c\/p\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:400px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eSample\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eMax per prep\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTotal RNA yield (µg)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eLiver\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e130\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePancreas\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eSpleen\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e15 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eBrain\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e120 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eLung\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e100 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e70\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eHeart\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e100 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eMuscle\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e80 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eKidney\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e20 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e45\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eTomato leaves\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e200 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eHeLa cells\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e5 × 10⁶\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e120\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eUsing more tissue than recommended can exceed the column's capacity, which lowers yield and raises genomic DNA carryover.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhat to expect from the RNA\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eSize range\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eRNA shorter than about 200 bases doesn't bind efficiently, so this kit isn't suited to miRNA or other small-RNA work.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eGenomic DNA\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eBiomiga notes that only trace genomic DNA remains. The optional on-column DNase I step removes it when your assay needs that. DNase I is not included; Biomiga sells it separately.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eGetting the most RNA\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-left:4px solid #2E7D5B;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px;color:#2E7D5B\"\u003eDo\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003eAdd 1% β-mercaptoethanol to Buffer LY before use, then keep it at 4 °C\u003c\/li\u003e\n\u003cli\u003eAdd ethanol to the RNA Wash Buffer before first use\u003c\/li\u003e\n\u003cli\u003eSnap-freeze tissue you can't process right away\u003c\/li\u003e\n\u003cli\u003eCheck RNA integrity on a denaturing gel before critical downstream work\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-left:4px solid #B3261E;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px;color:#B3261E\"\u003eDon't\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003eExceed the input in the table above\u003c\/li\u003e\n\u003cli\u003eSkip the lid-open dry spin\u003c\/li\u003e\n\u003cli\u003eLoad excess RNA into RT-PCR if genomic DNA signal is a problem; Biomiga suggests 50–100 ng\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003e100% ethanol, β-mercaptoethanol, a microcentrifuge, a tissue homogenizer (for tissue samples), RNase-free DNase I (optional), and pipettes with RNase-free filter tips.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eWhat's in the box\u003c\/h3\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto\"\u003e\u003ctable style=\"width:100%;min-width:260px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:8px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003e50 preps\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003e250 preps\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eBuffer LY\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e30 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e135 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eBuffer RB\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e30 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e135 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eRNA Wash Buffer (concentrate)\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eDEPC-treated water\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eRNA columns\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eBiomiga also lists collection tubes and 1.5 mL RNase-free microfuge tubes. The 4-prep trial contains the same components in trial quantities.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eBuilt for\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eGene expression\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eRT-qPCR from cell and tissue samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eTissue panels\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eInput guidance for ten sample types.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eNorthern and microarray\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTotal RNA in a small elution volume.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eMethod switching\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eValidate on your own samples with the 4-prep trial.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich Biomiga RNA kit do I need?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6311-gallery-3-kit-chooser.png?v=1791150743\" alt=\"Kit chooser: R6311 with RNA columns and optional DNase I versus R6321 Plus with gDNA clearance columns\" loading=\"lazy\" style=\"display:block;width:100%;max-width:640px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:640px;text-align:center\"\u003eDocumented kit contents differ; we don't compare yields between kits.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTHIS KIT\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003eCell \u0026amp; Tissue RNA Mini (R6311)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTotal RNA, with an optional DNase step for gDNA.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#13202E\"\u003eCell\/Tissue RNA Kit Plus (R6321)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eAdds a gDNA clearance column to remove genomic DNA without the DNase step.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e96-well Tissue RNA Kit (R6811)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eThe same job in a 96-well plate format.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e1st cDNA Synthesis Kit (FS01)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eThe next step after extraction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eNeed one of the others? Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a quote.\u003c\/p\u003e\n\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this kit is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eRoutine total RNA:\u003c\/strong\u003e cells and animal tissue for RT-qPCR, Northern or microarray.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003egDNA under your control:\u003c\/strong\u003e trace levels by default, with an on-column DNase step when needed.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eSimple storage:\u003c\/strong\u003e kit components store at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eLow-risk trial:\u003c\/strong\u003e 4 preps to test your own samples before committing to 50 or 250.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A silica spin-column kit for total RNA from cultured cells and animal tissue.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eElution:\u003c\/strong\u003e 50–100 µL DEPC-treated water.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eRNA size:\u003c\/strong\u003e RNA longer than about 200 nt is retained.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePack sizes:\u003c\/strong\u003e 4, 50 and 250 preps.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDoes it recover miRNA or other small RNAs?\u003c\/summary\u003e\u003cp\u003eNo. Biomiga notes that RNA shorter than about 200 bases doesn't bind the column efficiently, so this kit isn't suited to small-RNA work.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eIs the RNA free of genomic DNA?\u003c\/summary\u003e\u003cp\u003eNot guaranteed. Biomiga notes trace genomic DNA may remain. You can add the optional on-column DNase I step (DNase sold separately), or choose \u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#C2570C\"\u003eR6321 Plus\u003c\/a\u003e, which includes a gDNA clearance column.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat sample types does it handle?\u003c\/summary\u003e\u003cp\u003eSuspension and adherent cultured cells, and animal tissues such as liver, kidney, spleen, lung, heart, muscle, brain and pancreas. Biomiga's yield table also includes tomato leaves. Ask us before using other plant tissues.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat do I need to prepare before first use?\u003c\/summary\u003e\u003cp\u003eAdd 1% β-mercaptoethanol to Buffer LY and store it at 4 °C. Add 100% ethanol to the RNA Wash Buffer: 48 mL for the 50-prep kit, 200 mL for the 250-prep kit.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow long does a prep take?\u003c\/summary\u003e\u003cp\u003eBiomiga describes the method as taking about 30 minutes. The optional DNase step adds a 15-minute incubation, and tissue homogenization time varies by sample.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should the kit be stored?\u003c\/summary\u003e\u003cp\u003eKit components store at room temperature. Once β-mercaptoethanol is added, keep Buffer LY at 4 °C. Store purified RNA at −20 °C or colder. The expiry date is printed on the label.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow does it compare with the QIAGEN RNeasy Mini Kit?\u003c\/summary\u003e\u003cp\u003eBoth are silica spin-column kits for total RNA from cells and animal tissue, and both are designed around RNA longer than about 200 nucleotides, so neither is a small-RNA kit. Neither removes genomic DNA in its standard protocol without an optional DNase step. QIAGEN publishes a binding capacity of up to 100 µg per RNeasy Mini column; Biomiga's R6311 manual doesn't state a column capacity, so we don't compare capacity or yield. The two kits use different buffers and protocols, so validate R6311 on your own samples first; the 4-prep trial pack is sized for that. RNeasy and QIAGEN are trademarks of QIAGEN; Blue Tiger Scientific is not affiliated with QIAGEN.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan this kit be used for clinical or diagnostic work?\u003c\/summary\u003e\u003cp\u003eNo. It is intended for in vitro research use only and is not for use in diagnostic procedures or in humans.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"4 Preps","offer_id":52839300399402,"sku":"BTS-R6311-00","price":20.0,"currency_code":"USD","in_stock":true},{"title":"50 Preps","offer_id":52839302431018,"sku":"BTS-R6311-01","price":150.0,"currency_code":"USD","in_stock":true},{"title":"250 Preps","offer_id":52839302463786,"sku":"BTS-R6311-02","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6311-1.jpg?v=1791150241","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}