{"product_id":"biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321","title":"Biomiga Cell\/Tissue Total RNA Extraction Kit Plus, gDNA Removal Columns (R6321)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e\u0026lt;30 min\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eBiomiga-stated prep time\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e50–100 µL\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eElution volume\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e30 mg\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eMax tissue per prep (or 1 × 10⁷ cells)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e4 \/ 50 \/ 250\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003ePreps per kit\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eTOTAL RNA WITH gDNA REMOVAL\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eGenomic DNA cleared before the RNA ever reaches the column.\u003c\/h2\u003e\n\u003cp\u003eThe Biomiga Cell\/Tissue Total RNA Extraction Kit Plus (R6321) lyses samples in denaturing Buffer HLY, which Biomiga says immediately inhibits RNase activity. The lysate passes through a DNA Clearance column that traps genomic DNA. The flow-through is mixed with ethanol, bound to an ezBind RNA column, washed twice, and eluted in 50–100 µL of DEPC-treated water. Biomiga states the protocol takes under 30 minutes and describes the RNA as ready for NGS, RT-PCR, qPCR, differential display and microarrays.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture data-bts-r6321-inline=\"workflow\" style=\"display:block;margin:20px 0\"\u003e\u003csource media=\"(max-width:700px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6321-workflow-mobile.png?v=1791155408\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6321-workflow-desktop.png?v=1791155407\" alt=\"R6321 Plus overview: DNA clearance, transfer of RNA-containing flow-through, RNA capture and purification\" loading=\"lazy\" style=\"display:block;width:100%;max-width:980px;height:auto;margin:auto;border-radius:10px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:640px;text-align:center\"\u003eWorkflow overview; follow the R6321 user manual for volumes, speeds and buffer preparation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eLyse\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAdd 500 µL Buffer HLY (with β-mercaptoethanol added) and homogenize. Use 700 µL for more than 5 × 10⁶ cells or more than 15 mg tissue.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eClear gDNA\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eSpin the lysate through the DNA Clearance column. Keep the flow-through; the genomic DNA stays on the column.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eBind and wash\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAdd half a volume of 100% ethanol, bind to the ezBind RNA column, then wash with Buffer HRB and RNA Wash Buffer. Finish with a dry spin.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eElute\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eElute in 50–100 µL of DEPC-treated water. Biomiga notes that re-applying the eluate typically recovers another 20–30% of the RNA.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eFull protocol, volumes and speeds are in the user manual.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eInput and expected yield\u003c\/h2\u003e\n\u003cp\u003eBiomiga's reported total RNA yields per 10 mg of tissue or per 10⁶ cells. Yields vary with sample type, quality and handling.\u003c\/p\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:400px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eSample\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eInput\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eTotal RNA yield (µg)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eLiver\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eHeart\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eSpleen\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30–40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eKidney\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e20–30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eMuscle\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ePancreas\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eLung\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 mg\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10–20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eCOS-7 cells\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 × 10⁶\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eHeLa cells\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 × 10⁶\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eHEK293 \/ NIH 3T3 cells\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1 × 10⁶\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10–12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eDon't exceed 30 mg of tissue or 1 × 10⁷ cells per column; overloading lowers yield and lets genomic DNA through.\u003c\/p\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhat to expect from the RNA\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eSize range\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eRNA shorter than about 200 bases doesn't bind efficiently, so this kit isn't suited to miRNA or other small-RNA work.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eGenomic DNA\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eBiomiga states that the clearance column and RNA column together remove nearly all genomic DNA, so DNase digestion isn't needed for most applications. Sensitive applications may need an additional DNase I digest; Biomiga's DNase I (E11-01) is sold separately.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eGetting the most RNA\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-left:4px solid #2E7D5B;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px;color:#2E7D5B\"\u003eDo\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003eAdd β-mercaptoethanol (or 1 M DTT) to the Buffer HLY you'll use, as the manual describes; it then keeps up to 1 month at room temperature\u003c\/li\u003e\n\u003cli\u003eWarm Buffer HLY to 37 °C if crystals form\u003c\/li\u003e\n\u003cli\u003eAdd ethanol to the RNA Wash Buffer before first use\u003c\/li\u003e\n\u003cli\u003eRemove all culture medium before lysis\u003c\/li\u003e\n\u003cli\u003eStabilize tissue in RNAsecure solution or snap-freeze it\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;border:1px solid #DCE2E8;border-left:4px solid #B3261E;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px;color:#B3261E\"\u003eDon't\u003c\/h3\u003e\n\u003cul style=\"margin:0;padding-left:20px\"\u003e\n\u003cli\u003eExceed 30 mg tissue or 1 × 10⁷ cells per column\u003c\/li\u003e\n\u003cli\u003eSkip the dry spin before elution\u003c\/li\u003e\n\u003cli\u003eAdd bleach or acidic solutions to the preparation waste; Biomiga's manual notes Buffer HLY contains chaotropic salts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003e100% ethanol, β-mercaptoethanol (or 1 M DTT), a microcentrifuge, RNase-free 1.5 mL tubes and tips, and a homogenizer for tissue samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eWhat's in the box\u003c\/h3\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto\"\u003e\u003ctable style=\"width:100%;min-width:300px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:8px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003e4\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003e50\u003c\/th\u003e\n\u003cth style=\"padding:8px\"\u003e250 preps\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eBuffer HLY\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e4 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e150 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eBuffer HRB\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e4 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e50 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e150 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eRNA Wash Buffer (concentrate)\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e2 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e12 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eDEPC-treated water\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eezBind RNA columns\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:8px\"\u003eDNA Clearance columns\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"padding:8px\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eBiomiga also lists collection tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eBuilt for\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eNGS input\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eBiomiga lists NGS among the applications for this RNA.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eRT-qPCR\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003egDNA cleared on a column, without a separate DNase step for most assays.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eCell-line panels\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eYield guidance for HeLa, HEK293, NIH 3T3 and COS-7.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eTissue work\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eInput guidance for seven animal tissues.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich Biomiga RNA kit do I need?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture data-bts-r6321-inline=\"comparison\" style=\"display:block;margin:20px 0\"\u003e\u003csource media=\"(max-width:700px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6321-compare-mobile.png?v=1791155407\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6321-compare-desktop.png?v=1791155408\" alt=\"R6321 dedicated DNA-clearance column compared with R6311 optional-DNase workflow; sensitive R6321 applications may also require DNase\" loading=\"lazy\" style=\"display:block;width:100%;max-width:980px;height:auto;margin:auto;border-radius:10px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:640px;text-align:center\"\u003eDocumented kit contents differ; we don't compare yields between kits.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 200px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTHIS KIT\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:17px\"\u003eCell\/Tissue RNA Kit Plus (R6321)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTotal RNA with genomic DNA removed on a clearance column.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311\" style=\"color:#13202E\"\u003eCell \u0026amp; Tissue RNA Mini (R6311)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eNo gDNA column; an optional DNase step instead.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e96-well Tissue RNA Kit (R6811)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTissue RNA in a 96-well plate format.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 200px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003eRNAsecure Solution (R1011)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eStabilize RNA in harvested tissue before extraction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eNeed one of the others? Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a quote.\u003c\/p\u003e\n\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this kit is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eNo DNase step for most work:\u003c\/strong\u003e genomic DNA is removed on the clearance column; add DNase only for sensitive applications.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eReady for NGS and qPCR:\u003c\/strong\u003e applications listed by Biomiga for the purified RNA.\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eCell-line guidance:\u003c\/strong\u003e expected yields for four common lines.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eLow-risk trial:\u003c\/strong\u003e 4 preps to test your own samples before committing to 50 or 250.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A silica spin-column kit for total RNA from cultured cells and animal tissue, with a genomic DNA clearance column.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eElution:\u003c\/strong\u003e 50–100 µL DEPC-treated water.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eMax input:\u003c\/strong\u003e 30 mg tissue or 1 × 10⁷ cells per prep.\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePack sizes:\u003c\/strong\u003e 4, 50 and 250 preps.\u003c\/p\u003e\n\u003cp style=\"margin:0\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow is genomic DNA removed?\u003c\/summary\u003e\u003cp\u003eThe lysate passes through a DNA Clearance column that traps genomic DNA before the RNA binds to the ezBind RNA column. Biomiga notes the clearance column also helps homogenize the sample.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDo I still need a DNase step?\u003c\/summary\u003e\u003cp\u003eUsually not. Biomiga states the clearance column and RNA column together remove nearly all genomic DNA, so DNase digestion isn't necessary for most downstream applications. Some sensitive applications may need an additional DNase I digest; Biomiga's DNase I (E11-01) is sold separately.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDoes it recover miRNA or other small RNAs?\u003c\/summary\u003e\u003cp\u003eNo. Biomiga notes that RNA shorter than about 200 bases doesn't bind the column efficiently, so this kit isn't suited to small-RNA work.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat samples can I use, and how much?\u003c\/summary\u003e\u003cp\u003eCultured cells (suspension or adherent) and animal tissue. Use 500 µL Buffer HLY for up to 5 × 10⁶ cells or 15 mg tissue, and 700 µL for up to 1 × 10⁷ cells or 30 mg tissue. Don't exceed 1 × 10⁷ cells or 30 mg tissue per column; overloading causes genomic DNA carryover.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eWhat do I need to prepare before first use?\u003c\/summary\u003e\u003cp\u003eAdd β-mercaptoethanol (or 1 M DTT) to the volume of Buffer HLY you'll use, as described in the user manual; once added, Buffer HLY keeps at room temperature for up to 1 month. Add 100% ethanol to the RNA Wash Buffer: 8 mL for the 4-prep kit, 48 mL for the 50-prep kit, or 200 mL for the 250-prep kit. If crystals form in Buffer HLY, dissolve them at 37 °C before use.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow long does a prep take?\u003c\/summary\u003e\u003cp\u003eBiomiga states the protocol can be completed in under 30 minutes. Tissue homogenization time varies by sample.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should the kit be stored and handled?\u003c\/summary\u003e\u003cp\u003eAll components store at room temperature. Store purified RNA at −20 °C or colder. The expiry date is printed on the label. Biomiga's manual advises gloves and eye protection when handling Buffers HLY and HRB, and not adding bleach or acidic solutions to the preparation waste.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow does it compare with the QIAGEN RNeasy Plus Mini Kit?\u003c\/summary\u003e\u003cp\u003eBoth are silica spin-column kits that remove genomic DNA on a dedicated column before RNA binding. Both retain RNA longer than about 200 nucleotides, and both list a maximum of 1 × 10⁷ cells or 30 mg tissue per prep. QIAGEN publishes up to 100 µg total RNA per RNeasy Plus Mini column and a protocol under 25 minutes. Biomiga states under 30 minutes for R6321 and doesn't publish a column capacity, so we don't compare capacity or yield. Buffers and protocols differ, so validate on your own samples; the 4-prep trial is sized for that. RNeasy and QIAGEN are trademarks of QIAGEN; Blue Tiger Scientific is not affiliated with QIAGEN.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan this kit be used for clinical or diagnostic work?\u003c\/summary\u003e\u003cp\u003eNo. It is intended for in vitro research use only and is not for use in diagnostic procedures or in humans.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"4 Preps","offer_id":52839468761386,"sku":"BTS-R6321-00","price":20.0,"currency_code":"USD","in_stock":true},{"title":"50 Preps","offer_id":52839469187370,"sku":"BTS-R6321-01","price":190.0,"currency_code":"USD","in_stock":true},{"title":"250 Preps","offer_id":52839469220138,"sku":"BTS-R6321-02","price":780.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/R6321-1.jpg?v=1791152115","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}