{"product_id":"biomiga-1st-strand-cdna-synthesis-kit-fs01","title":"Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) \u0026 Hexamer (FS01)","description":"\u003cdiv style=\"font-family:inherit;color:#13202E;line-height:1.6;max-width:1000px;margin:0 auto;width:100%;box-sizing:border-box;overflow-wrap:anywhere\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:1px;background:#24425F;border-radius:12px;overflow:hidden;margin:0 0 clamp(28px,6vw,40px)\"\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;color:#F2A65A;white-space:nowrap;line-height:1.2\"\u003e10 pg–5 µg\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eTotal RNA input\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e20 µL\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eReaction volume\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003edT + hexamer\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003ePrimers included\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 140px;background:#0E2A47;padding:clamp(14px,4vw,20px);color:#fff;box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:clamp(21px,6vw,30px);font-weight:700;white-space:nowrap;line-height:1.2\"\u003e2 years\u003c\/div\u003e\n\u003cdiv style=\"font-size:14px;color:#C9D5E1;line-height:1.35;margin-top:4px\"\u003eStability at −20 °C\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eTWO-STEP RT-PCR · cDNA SYNTHESIS\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrom RNA to PCR-ready cDNA in about an hour.\u003c\/h2\u003e\n\u003cp\u003eBiomiga's 1st Strand cDNA Synthesis Kit (FS01) converts total or poly(A)+ RNA into first-strand cDNA. Its reverse transcriptase mix combines an engineered MMLV reverse transcriptase with a ribonuclease inhibitor protein, and the kit supplies both oligo(dT)\u003csub\u003e20\u003c\/sub\u003e and random hexamer annealing buffers. Biomiga states the system works from 10 pg to 5 µg of total RNA and detects RNA targets from 100 bp to 10 kb, and that it is suited to gene isolation, high-throughput expression profiling by real-time quantitation, and end-point RT-PCR.\u003c\/p\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:0\"\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eHow it works\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/FS01-inline-workflow-mobile.png?v=1791231026\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/FS01-inline-workflow-desktop.png?v=1791231025\" alt=\"First-strand cDNA synthesis workflow for Biomiga FS01: anneal primer at 65 °C, add RT mix, incubate at 37 °C for 50 min, use 1–2 µL in PCR or qPCR\" width=\"1720\" height=\"908\" loading=\"lazy\" style=\"display:block;width:100%;max-width:860px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:860px;text-align:center\"\u003eBiomiga's 1st-strand protocol, 20 µL per reaction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 1\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAnneal primer\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eCombine RNA with 5 µL annealing buffer OT (oligo(dT)\u003csub\u003e20\u003c\/sub\u003e) or RH (random hexamer), water to 10 µL. Heat at 65 °C for 3 min, then place on ice.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 2\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eAdd RT mix\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eAdd 10 µL of reaction mixture (9 µL cDNA synthesis buffer + 1 µL RT and RNase inhibitor mix). Mix gently and spin briefly.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 3\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eReverse transcribe\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003e37 °C for 50 min. With random hexamers, incubate 5 min at room temperature (22–24 °C) first.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 210px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eSTEP 4\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0;font-size:18px\"\u003eUse or store\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eOptionally heat to 85 °C for 5 min. Use 1–2 µL of the 20 µL reaction per PCR; store the rest at −20 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eReaction setup\u003c\/h2\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:420px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eRNA\/primer mix (10 µL)\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eOligo(dT)\u003csub\u003e20\u003c\/sub\u003e\n\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003eRandom hexamer\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eRNA (10 pg–5 µg)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003ex µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003ex µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eAnnealing buffer OT\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e5.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eAnnealing buffer RH\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e—\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e5.0 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 10 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003eto 10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv style=\"height:12px\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:360px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eRT reaction mixture\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e1 reaction\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e10 reactions\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ecDNA synthesis buffer\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e9.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e90 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eRT and RNase inhibitor mix\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eTotal\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e10 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eFor mRNA targets under 1.5 kb, a 30-minute incubation is an option. RT temperature can be optimized between 37 and 42 °C. Heat inactivation at 85 °C is recommended when using less than 10 ng of RNA.\u003c\/p\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px;margin:16px 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eNo-RT control\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eFor accurate expression profiling, run the same amount of RNA without reverse transcriptase. Any signal in that control points to genomic DNA contamination.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 240px;border:1px solid #DCE2E8;border-top:4px solid #0E2A47;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:17px\"\u003eDNase option\u003c\/h3\u003e\n\u003cp style=\"margin:0\"\u003eIf the no-RT control shows product, Biomiga describes an optional DNase I treatment before cDNA synthesis. DNase I and its buffer are not included.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eWhich kit do I need?\u003c\/h2\u003e\n\u003cdiv style=\"margin:0 0 16px;width:100%\"\u003e\n\u003cpicture\u003e\u003csource media=\"(max-width:640px)\" srcset=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/FS01-inline-chooser-mobile.png?v=1791231025\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/FS01-inline-chooser-desktop.png?v=1791231026\" alt=\"Two-step or one-step: Biomiga FS01 1st Strand cDNA Synthesis Kit highlighted, compared with the RT0201 One-Step RT-PCR Kit\" width=\"1720\" height=\"1050\" loading=\"lazy\" style=\"display:block;width:100%;max-width:860px;height:auto;margin:0 auto;border-radius:12px\"\u003e\u003c\/picture\u003e\u003cp style=\"font-size:14px;color:#5B6B7C;margin:8px auto 0;max-width:860px;text-align:center\"\u003eBoth kits start from purified RNA; they differ in how the cDNA is made and used.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 220px;border:2px solid #C2570C;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eTHIS KIT\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0 6px;font-size:17px\"\u003e1st Strand cDNA Synthesis Kit (FS01)\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eMakes a reusable cDNA pool with oligo(dT) or random hexamer priming, for qPCR or end-point PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003cdiv style=\"font-size:13px;font-weight:600;color:#C2570C\"\u003eONE-STEP\u003c\/div\u003e\n\u003ch3 style=\"margin:6px 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-one-step-rt-pcr-kit-rt0201\" style=\"color:#13202E\"\u003eOne-Step RT-PCR Kit (RT0201)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eRT and PCR in one tube with your own primers; end-point product for gel analysis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:12px 0 0\"\u003eFor quantitative results, run FS01 cDNA with a Biomiga SYBR Green qPCR mix matched to your instrument: \u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-no-rox-qp1311\" style=\"color:#C2570C\"\u003eno ROX (QP1311)\u003c\/a\u003e, \u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-low-rox-qp1312\" style=\"color:#C2570C\"\u003elow ROX (QP1312)\u003c\/a\u003e or \u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-rox-qp1313\" style=\"color:#C2570C\"\u003eROX (QP1313)\u003c\/a\u003e.\u003c\/p\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:clamp(20px,5vw,32px);margin:clamp(28px,6vw,40px) 0 0\"\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0\"\u003e\n\u003cp style=\"font-size:13px;font-weight:600;letter-spacing:.12em;color:#C2570C;margin:0 0 6px\"\u003eBEFORE YOU START\u003c\/p\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eYou supply\u003c\/h2\u003e\n\u003cp style=\"margin:0\"\u003ePurified RNA (for example from the \u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311\" style=\"color:#C2570C\"\u003eR6311\u003c\/a\u003e or \u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#C2570C\"\u003eR6321\u003c\/a\u003e kits), PCR or qPCR reagents for the next step, and optional RNase-free DNase I.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 280px;min-width:0;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 8px;font-size:18px\"\u003eIn the box\u003c\/h3\u003e\n\u003cdiv style=\"width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch\"\u003e\u003ctable style=\"width:100%;min-width:330px;border-collapse:collapse;font-size:15px\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#EEF2F6;text-align:left\"\u003e\n\u003cth style=\"padding:10px\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e20 rxn\u003c\/th\u003e\n\u003cth style=\"padding:10px\"\u003e50 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eRT and RNase inhibitor mix\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e20 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003ecDNA synthesis buffer\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e250 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eAnnealing buffer OT (oligo(dT)\u003csub\u003e20\u003c\/sub\u003e)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e125 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e250 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eAnnealing buffer RH (random hexamer)\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e125 µL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e250 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-top:1px solid #E4E9EE\"\u003e\n\u003ctd style=\"padding:10px\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.0 mL\u003c\/td\u003e\n\u003ctd style=\"padding:10px\"\u003e1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:14px;color:#5B6B7C;margin:10px 0 0\"\u003eThe 5- and 100-reaction packs contain the same five components in 5- and 100-reaction quantities.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background:#0E2A47;color:#fff;border-radius:12px;padding:clamp(18px,5vw,28px);margin:clamp(28px,6vw,40px) 0 0;box-sizing:border-box\"\u003e\n\u003ch2 style=\"margin:0 0 12px;font-size:clamp(20px,5vw,26px);line-height:1.25;color:#fff\"\u003eWhen this kit is the right call\u003c\/h2\u003e\n\u003cul style=\"margin:0;padding-left:20px;color:#E3EAF1\"\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eMany targets, one RNA sample:\u003c\/strong\u003e make a cDNA pool once and run as many PCR or qPCR assays as you need\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eYour choice of priming:\u003c\/strong\u003e oligo(dT)\u003csub\u003e20\u003c\/sub\u003e for poly(A) mRNA or random hexamers for broader coverage\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eWide input range:\u003c\/strong\u003e 10 pg to 5 µg total RNA per reaction, per Biomiga\u003c\/li\u003e\n\u003cli style=\"margin-bottom:6px\"\u003e\n\u003cstrong style=\"color:#F2A65A\"\u003eTrial size:\u003c\/strong\u003e the 5-reaction pack lets you test it on your own samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eRelated Biomiga products\u003c\/h2\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;gap:12px\"\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-spin-column-r6311\" style=\"color:#13202E\"\u003eCell \u0026amp; Tissue RNA Mini Kit (R6311)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eTotal RNA from cells and tissue.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-cell-tissue-total-rna-extraction-kit-plus-gdna-removal-columns-r6321\" style=\"color:#13202E\"\u003eCell\/Tissue RNA Kit Plus (R6321)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eAdds a gDNA clearance column.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-sybr-green-qpcr-master-mix-no-rox-qp1311\" style=\"color:#13202E\"\u003eSYBR Green qPCR Master Mixes (QP1311–QP1313)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eQuantify your cDNA by real-time PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex:1 1 220px;border:1px solid #DCE2E8;border-radius:12px;padding:clamp(14px,4vw,18px);box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 6px;font-size:17px\"\u003e\u003ca href=\"\/products\/biomiga-express-hi-fi-dna-polymerase-ehf1101\" style=\"color:#13202E\"\u003eExpress Hi Fi DNA Polymerase (EHF1101)\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eHigh-fidelity PCR from cDNA for cloning.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"margin:clamp(28px,6vw,40px) 0 16px;font-size:clamp(22px,5.5vw,28px);line-height:1.25\"\u003eFrequently asked questions\u003c\/h2\u003e\n\u003cdiv style=\"border:1px solid #DCE2E8;border-radius:12px;padding:clamp(16px,4vw,20px);margin:0 0 16px;box-sizing:border-box\"\u003e\n\u003ch3 style=\"margin:0 0 10px;font-size:17px\"\u003eQuick facts\u003c\/h3\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eWhat it is:\u003c\/strong\u003e A first-strand cDNA synthesis system with an engineered MMLV RT, RNase inhibitor, and oligo(dT)\u003csub\u003e20\u003c\/sub\u003e and random hexamer primers\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eRNA input:\u003c\/strong\u003e 10 pg–5 µg total RNA per 20 µL reaction\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003ePacks:\u003c\/strong\u003e 5, 20, 50 or 100 reactions\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e −20 °C (non-frost-free); stable 2 years\u003c\/p\u003e\n\u003cp style=\"margin:0 0 6px\"\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e In vitro research use only\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eShould I use oligo(dT) or random hexamers?\u003c\/summary\u003e\u003cp\u003eOligo(dT)\u003csub\u003e20\u003c\/sub\u003e primes from the poly(A) tail of mRNA. Random hexamers prime throughout the RNA, including transcripts without a poly(A) tail. Both annealing buffers are included, so you can choose per experiment.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow much RNA can I use?\u003c\/summary\u003e\u003cp\u003eFrom 10 pg to 5 µg of total RNA per 20 µL reaction, per Biomiga. Poly(A)+ purified RNA can also be used.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow long does it take?\u003c\/summary\u003e\u003cp\u003eAbout an hour: 3 minutes at 65 °C to anneal, then 50 minutes at 37 °C (plus 5 minutes at room temperature first with random hexamers). For mRNA targets under 1.5 kb, 30 minutes at 37 °C is an option.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow much cDNA goes into a PCR?\u003c\/summary\u003e\u003cp\u003eBiomiga recommends 1–2 µL of the 20 µL first-strand reaction per PCR. Store the rest at −20 °C.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eDo I need to heat-inactivate the RT?\u003c\/summary\u003e\u003cp\u003eIt is optional. Biomiga recommends 85 °C for 5 minutes when the reaction used less than 10 ng of RNA.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow do I check for genomic DNA contamination?\u003c\/summary\u003e\u003cp\u003eRun a no-RT control with the same amount of RNA but no reverse transcriptase. If it gives a signal, treat the RNA with RNase-free DNase I before cDNA synthesis, or start from an RNA kit with gDNA removal such as R6321.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eIs DNase I included?\u003c\/summary\u003e\u003cp\u003eNo. Biomiga describes an optional DNase I step, but DNase I and its reaction buffer are supplied by you.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eShould I choose this kit or the One-Step RT-PCR Kit?\u003c\/summary\u003e\u003cp\u003eChoose FS01 when you want a cDNA pool to use in several assays or in qPCR. Choose RT0201 when you want to go from RNA to an end-point PCR product in one tube.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan I switch from another vendor's cDNA synthesis kit?\u003c\/summary\u003e\u003cp\u003eUsually, but Biomiga has not published side-by-side data. Run a small pilot with a no-RT control before switching an established assay.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eHow should the kit be stored?\u003c\/summary\u003e\u003cp\u003eAt −20 °C in a non-frost-free freezer. Biomiga states the kit is stable for 2 years when stored this way.\u003c\/p\u003e\u003c\/details\u003e\u003cdetails style=\"border-bottom:1px solid #DCE2E8;padding:14px 0\"\u003e\u003csummary style=\"font-weight:600;cursor:pointer;padding:4px 0\"\u003eCan my institution get a formal quote?\u003c\/summary\u003e\u003cp\u003eYes. Email \u003ca href=\"mailto:info@bluetigerscientific.com\" style=\"color:#C2570C\"\u003einfo@bluetigerscientific.com\u003c\/a\u003e or text \u003ca href=\"tel:833-928-4437\" style=\"color:#C2570C\"\u003e(833) 928-4437\u003c\/a\u003e for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.\u003c\/p\u003e\u003c\/details\u003e\u003cp style=\"font-size:12px;color:#7A8896;margin:16px 0 0\"\u003eInstrument and product names are trademarks of their respective owners.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Biomiga","offers":[{"title":"5 reactions","offer_id":52851497206058,"sku":"BTS-FS01-00","price":40.0,"currency_code":"USD","in_stock":true},{"title":"20 reactions","offer_id":52851497238826,"sku":"BTS-FS01-01","price":150.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions","offer_id":52851497271594,"sku":"BTS-FS01-02","price":320.0,"currency_code":"USD","in_stock":true},{"title":"100 reactions","offer_id":52851497304362,"sku":"BTS-FS01-03","price":560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Biomiga1stStrandcDNASynthesisKit_Oligo_dT_Hexamer_FS01.jpg?v=1791239111","url":"https:\/\/bluetigerscientific.com\/products\/biomiga-1st-strand-cdna-synthesis-kit-fs01","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}