{"product_id":"beads-stabilizer-tris","title":"Beads Stabilizer (TRIS)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eBeads Stabilizer (TRIS) – Catalog #B2025671\u003c\/h2\u003e\n\u003cp\u003eBeads Stabilizer (TRIS) is a buffered solution designed to preserve the activity and binding capacity of magnetic and agarose beads during storage and between use cycles. Supplied as 50 mL of TRIS (tromethamine)-buffered solution, this stabilizer is widely used in biochemical workflows where pH stability and minimal interference with enzyme reactions are important.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025671\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eTRIS-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003epH:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e~8.0 (adjustable to pH 7–9)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBead maintenance and storage, affinity purification, enzyme assays, pull-down assays, magnetic bead separation, protein binding workflows\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBeads stabilizer, TRIS buffer, tromethamine buffer, TRIS solution, magnetic beads maintenance, agarose beads stabilizer, buffer solution, bead stabilization, Tris-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eTRIS (2-amino-2-hydroxymethyl-propane-1,3-diol or tromethamine) is a widely used zwitterionic buffer effective across pH 7–9, with buffering capacity typically centered around pH 8.0. Unlike phosphate buffers, TRIS does not chelate divalent metal cations and does not react with aldehydes, making it ideal for enzyme assays, protein purification, and binding workflows where these interactions must be preserved.\u003c\/p\u003e\n\u003cp\u003eThe TRIS-buffered formulation of this bead stabilizer is particularly useful when beads must be stored or used in applications requiring higher pH conditions, or when phosphate-related precipitation or interference is a concern.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eStoring magnetic and agarose beads between use cycles\u003c\/li\u003e\n\u003cli\u003eBuffer for enzyme-coupled assays and kinetic studies\u003c\/li\u003e\n\u003cli\u003eAffinity purification workflows requiring minimal metal ion chelation\u003c\/li\u003e\n\u003cli\u003ePull-down and immunoprecipitation assays at physiological to slightly alkaline pH\u003c\/li\u003e\n\u003cli\u003ePrevention of bead aggregation during extended storage\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the stabilizer at 2–8°C. Before use, allow refrigerated solutions to warm to room temperature. For bead storage, resuspend beads in this buffer at a concentration suitable for your application.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003epH adjustment:\u003c\/strong\u003e TRIS buffers can be adjusted by changing the ratio of TRIS-HCl (acidic form) to TRIS base (free base) or by adding HCl or NaOH. Typical working range is pH 7–9.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMetal ion compatibility:\u003c\/strong\u003e Unlike EDTA or phosphate buffers, TRIS does not chelate Mg²⁺, Ca²⁺, or Zn²⁺, preserving their availability in enzyme reactions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSterile use:\u003c\/strong\u003e If required, filter the beads in this buffer through 0.22 µm or sterilize by autoclaving at 121°C, 15 psi for 15 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGentle handling:\u003c\/strong\u003e Resuspend beads by inverting the tube several times; extended vortexing may cause aggregation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e50 mL of TRIS-buffered solution\u003c\/li\u003e\n\u003cli\u003epH ~8.0 (adjustable to 7–9 range)\u003c\/li\u003e\n\u003cli\u003eNon-chelating buffer suitable for enzyme assays and metal-ion-dependent reactions\u003c\/li\u003e\n\u003cli\u003eReady-to-use or autoclavable for sterile applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMaintains bead integrity and prevents aggregation during storage\u003c\/li\u003e\n\u003cli\u003eDoes not chelate divalent cations needed in enzyme assays\u003c\/li\u003e\n\u003cli\u003eCompatible with a wide range of pH conditions (pH 7–9)\u003c\/li\u003e\n\u003cli\u003eMinimizes background interference in protein binding workflows\u003c\/li\u003e\n\u003cli\u003eIndustry standard for enzyme kinetics and biochemical assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the pH of this TRIS solution?\u003c\/strong\u003e\u003cbr\u003eApproximately 8.0; TRIS buffers are stable across pH 7–9 and can be adjusted by changing the ratio of acid to base form.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow does TRIS differ from phosphate buffer?\u003c\/strong\u003e\u003cbr\u003eTRIS does not chelate divalent cations (Mg²⁺, Ca²⁺, Zn²⁺), does not react with aldehydes, and provides excellent buffering capacity in the pH 7–9 range, making it preferred for enzyme assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this for bead storage?\u003c\/strong\u003e\u003cbr\u003eYes. Resuspend beads in this buffer at an appropriate concentration and store at 2–8°C. TRIS is compatible with most bead types used in affinity purification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs temperature control critical?\u003c\/strong\u003e\u003cbr\u003eTRIS buffer is somewhat temperature-sensitive; its pH changes by ~0.03 units per degree Celsius. Account for this in temperature-critical applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat additives are compatible with TRIS?\u003c\/strong\u003e\u003cbr\u003eYou can add NaCl for osmotic stability, MgCl₂ or CaCl₂ for enzyme cofactors, and detergents such as Triton X-100 or Tween-20 for non-ionic washing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGumerova NI, Rompel A. More Than a Buffer in Biochemistry: Tris as an Architect and Gatekeeper of Metal-Oxo Assembly. \u003cem\u003eAngew Chem Int Ed Engl.\u003c\/em\u003e 2026;65(13):e25355.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/anie.202525355\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eRoush AE, Riaz M, Misra SK, Weinberger SR, Sharp JS. Intrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(hydroxymethyl)aminomethane. \u003cem\u003eJ Am Soc Mass Spectrom.\u003c\/em\u003e 2020;31(2):169-172.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/jasms.9b00088\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eShi SR, Cote RJ, Young L, Imam SA, Taylor CR. Use of pH 9.5 Tris-HCl buffer containing 5% urea for antigen retrieval immunohistochemistry. \u003cem\u003eBiotech Histochem.\u003c\/em\u003e 1996;71(4):190-6.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3109\/10520299609117158\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809017000234,"sku":"BTS-B2025671","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025671.png?v=1790859385","url":"https:\/\/bluetigerscientific.com\/products\/beads-stabilizer-tris","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}