{"product_id":"beads-stabilizer-pbs-protein-free","title":"Beads Stabilizer (PBS, Protein-free)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eBeads Stabilizer (PBS, Protein-free) – Catalog #B2025657\u003c\/h2\u003e\n\u003cp\u003eBeads Stabilizer (PBS, Protein-free) is a buffered solution designed to preserve the activity and binding capacity of magnetic and agarose beads during storage and between use cycles. Supplied as 50 mL of isotonic phosphate-buffered saline without added proteins, this formulation is particularly valuable in workflows where protein interference could compromise assay specificity or binding efficiency.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025657\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePhosphate-buffered saline (PBS), protein-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003epH:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e~7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBead maintenance and storage, affinity purification, immunoprecipitation, magnetic bead separation, protein binding assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBeads stabilizer, PBS buffer, phosphate-buffered saline, protein-free buffer, magnetic beads maintenance, agarose beads stabilizer, buffer solution, bead stabilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePhosphate-buffered saline (PBS) is an isotonic, physiological buffer system widely used in cell biology and immunological assays. At pH 7.4, PBS mimics the ionic strength and osmotic pressure of cellular fluid, making it ideal for maintaining the structural integrity of beads and preserving the function of immobilized or captured biomolecules during storage.\u003c\/p\u003e\n\u003cp\u003eThe protein-free formulation of this stabilizer is critical for applications where added proteins could compete for binding sites on the beads or interfere with detection. This is especially important in affinity chromatography, immunoprecipitation, and pull-down assays where background signal from excess protein must be minimized.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMaintaining magnetic and agarose beads between use cycles\u003c\/li\u003e\n\u003cli\u003eBuffer for bead-based affinity purification workflows\u003c\/li\u003e\n\u003cli\u003eStorage medium for coated or antibody-conjugated beads\u003c\/li\u003e\n\u003cli\u003eAssay buffer for protein-binding and separation applications\u003c\/li\u003e\n\u003cli\u003ePrevention of bead aggregation during storage\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the stabilizer at 2–8°C. Before use, allow refrigerated solutions to warm to room temperature. For bead storage, resuspend beads in this buffer at a concentration suitable for your application, then store at 2–8°C or at room temperature depending on bead type and desired shelf life.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotonicity:\u003c\/strong\u003e This PBS formulation is balanced to match osmotic conditions of cells and tissues; use without further dilution for bead suspension.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSterile use:\u003c\/strong\u003e If required, filter the beads in this buffer through 0.22 µm or sterilize by autoclaving at 121°C, 15 psi for 15 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAvoiding aggregation:\u003c\/strong\u003e Mix gently when resuspending beads; extended vortexing may cause aggregation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e50 mL of phosphate-buffered saline (PBS) without added proteins\u003c\/li\u003e\n\u003cli\u003epH ~7.4 isotonic buffer suitable for bead maintenance\u003c\/li\u003e\n\u003cli\u003eProtein-free formulation to minimize background in binding assays\u003c\/li\u003e\n\u003cli\u003eReady-to-use or autoclavable for sterile applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMaintains bead integrity and prevents aggregation during storage\u003c\/li\u003e\n\u003cli\u003eProtein-free formulation eliminates background interference\u003c\/li\u003e\n\u003cli\u003ePhysiologically relevant pH and osmotic conditions\u003c\/li\u003e\n\u003cli\u003eCompatible with downstream affinity and binding workflows\u003c\/li\u003e\n\u003cli\u003eCost-effective for large-scale bead-based applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the pH of this PBS solution?\u003c\/strong\u003e\u003cbr\u003eApproximately 7.4, designed to match physiological conditions and maintain bead functionality.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy is the protein-free formulation important?\u003c\/strong\u003e\u003cbr\u003eAdded proteins such as BSA can compete for binding sites on beads or interfere with detection assays. The protein-free formulation is ideal for affinity purification and pull-down assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this for long-term storage of beads?\u003c\/strong\u003e\u003cbr\u003eYes. Store at 2–8°C. For very long-term storage, consider adding 0.02% sodium azide as a preservative, or prepare fresh stocks periodically.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs this solution sterile?\u003c\/strong\u003e\u003cbr\u003eIt is sterile-filtered (0.22 µm). For critical applications, you may further autoclave the beads in this buffer at 121°C, 15 psi for 15 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I modify the buffer?\u003c\/strong\u003e\u003cbr\u003eFor specialized applications, this buffer can be supplemented with additives such as divalent cations (Mg²⁺, Ca²⁺) or detergents; consult relevant protocols for your specific assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHeng BC, Cowan CM, Basu S. Temperature and calcium ions affect aggregation of mesenchymal stem cells in phosphate buffered saline. \u003cem\u003eCytotechnology.\u003c\/em\u003e 2008;58(2):69-75.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s10616-008-9174-8\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eAabling RR, Alstrup T, Kjær EM, Poulsen KJ, Pedersen JO, Revenfeld AL, Møller BK, Eijken M. Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants. \u003cem\u003eRegen Ther.\u003c\/em\u003e 2023;23:67-75.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.reth.2023.03.006\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBecker I, Strauch SM, Hauslage J, Lebert M. Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects. \u003cem\u003eLife Sci Space Res (Amst).\u003c\/em\u003e 2017;13:12-18.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.lssr.2017.02.001\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809016803626,"sku":"BTS-B2025657","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025657.png?v=1790859374","url":"https:\/\/bluetigerscientific.com\/products\/beads-stabilizer-pbs-protein-free","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}