{"product_id":"atpa-magnetic-beads","title":"ATPA Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eATPA Magnetic Beads – Catalog #B2025748\u003c\/h2\u003e\n\u003cp\u003eATPA Magnetic Beads are affinity purification reagents combining a superparamagnetic iron oxide core with ATPA-based surface chemistry for selective capture of target biomolecules. This product is designed to facilitate rapid, one-step separation of bound targets from complex mixtures using a standard magnetic separator.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025748\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity-based isolation and purification of biomolecules, selective capture from complex mixtures, sample preparation for downstream analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eATPA magnetic beads, affinity purification beads, magnetic particle separation, selective capture, biomolecule isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eMagnetic bead-based purification is a standard technique in molecular biology and biochemistry for rapid, scalable separation of target molecules from complex samples. The ATPA-coated beads provide selective affinity interaction with target biomolecules while minimizing non-specific adsorption. The superparamagnetic iron oxide core responds rapidly to external magnetic fields, enabling quick pelleting and separation without centrifugation.\u003c\/p\u003e\n\u003cp\u003eThis magnetic format offers practical advantages in high-throughput and automated workflows: beads can be captured in seconds, wash steps are rapid and thorough, and elution conditions are mild, preserving protein activity and integrity. The 10 mL volume provided in this product supports both small-scale optimization and larger batch purifications.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSelective capture and isolation of target biomolecules\u003c\/li\u003e\n\u003cli\u003eRapid purification from cell lysates and complex mixtures\u003c\/li\u003e\n\u003cli\u003eSample preparation for proteomics and bioanalytical workflows\u003c\/li\u003e\n\u003cli\u003eBatch processing and automation-compatible purification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eResuspend beads gently (do not vortex) before use. Add beads to your sample in appropriate binding buffer and incubate with gentle rotation to allow binding to occur. Apply a magnet to pellet beads and remove supernatant. Perform 3–5 washes with ice-cold wash buffer, then elute bound material with a suitable elution buffer (acidic pH, high salt, or denaturing conditions) depending on your downstream application.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding conditions:\u003c\/strong\u003e Optimize pH, ionic strength, and incubation time according to your target properties and the manufacturer's guidance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Remove non-specific binding with detergent-containing buffers or high-salt washes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Choose elution conditions compatible with your downstream analysis; refer to the lot-specific technical data sheet for recommended protocols.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e10 mL of ATPA magnetic beads, ready to use\u003c\/li\u003e\n\u003cli\u003eSelective affinity capture of target biomolecules\u003c\/li\u003e\n\u003cli\u003eRapid, magnet-based separation\u003c\/li\u003e\n\u003cli\u003eScalable format suitable for optimization and batch processing\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMagnetic format enables hands-free, rapid separation\u003c\/li\u003e\n\u003cli\u003eNo centrifugation required, reducing sample loss\u003c\/li\u003e\n\u003cli\u003eLarge volume (10 mL) suitable for extensive experiments or optimization\u003c\/li\u003e\n\u003cli\u003eCompatible with high-throughput and automated workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is ATPA chemistry?\u003c\/strong\u003e\u003cbr\u003eFor a detailed description of ATPA and its binding characteristics, request the technical data sheet or contact us with your specific application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the binding capacity of these beads?\u003c\/strong\u003e\u003cbr\u003eBinding capacity depends on your specific target and assay conditions. Perform a titration experiment with your target molecule to determine optimal bead concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in an automated system?\u003c\/strong\u003e\u003cbr\u003eYes. The magnetic format is well suited to robotic sample processing; consult the technical data sheet for specific compatibility and protocol details.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat are recommended elution conditions?\u003c\/strong\u003e\u003cbr\u003eElution conditions vary depending on the target and desired downstream use. Refer to the lot-specific technical data sheet for recommended pH, salt, and buffer compositions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long can I incubate the beads with my sample?\u003c\/strong\u003e\u003cbr\u003eIncubation time may range from 15 minutes to several hours depending on target concentration and binding kinetics. Optimize for your application; longer incubation may improve recovery but may also increase background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eBeads can be regenerated and reused if thoroughly washed and stored properly; consult the technical data sheet for reuse protocols.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eTsunehiro M, Meki Y, Matsuoka K, Kinoshita-Kikuta E, Kinoshita E, Koike T. A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules. \u003cem\u003eJ Chromatogr B Analyt Technol Biomed Life Sci.\u003c\/em\u003e 2013;925:86-94.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jchromb.2013.02.039\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFujioka H, Tsunehiro M, Kawaguchi M, Kuramoto Y, Kurosaki H, Hieda Y, Kinoshita-Kikuta E, Kinoshita E, Koike T. Simple enrichment of thiol-containing biomolecules by using zinc(II)-cyclen-functionalized magnetic beads. \u003cem\u003eJ Sep Sci.\u003c\/em\u003e 2014;37(13):1601-9.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/jssc.201400135\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eShami-Shah A, Travis BG, Walt DR. Advances in extracellular vesicle isolation methods: a path towards cell-type specific EV isolation. \u003cem\u003eExtracell Vesicles Circ Nucl Acids.\u003c\/em\u003e 2023;4(3):447-460.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.20517\/evcna.2023.14\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809017393450,"sku":"BTS-B2025748","price":2475.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025748.png?v=1790859406","url":"https:\/\/bluetigerscientific.com\/products\/atpa-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}