{"product_id":"apex2-antibody-center-research-use-only-non-medical-copy","title":"APEX2 Antibody (Center) – Research Use Only – Non Medical","description":"\u003cdiv style=\"max-width: 1400px; margin: 0 auto; padding: 40px 20px; font-family: 'Open Sans',sans-serif; font-weight: 300; background: #fff; color: #333; font-size: 0.95rem; box-sizing: border-box;\"\u003e\n\u003cdiv style=\"display: flex; flex-direction: column; gap: 20px;\"\u003e\n\u003ch2 style=\"margin: 0; font-weight: 600;\"\u003eAPEX2 Antibody (Center) – Rabbit Polyclonal Antibody\u003c\/h2\u003e\n\u003cp\u003eAPEX2 Antibody (Center) (Catalog #L2002405) is a high-quality rabbit polyclonal antibody supplied as 100 µg of solution (0.25–0.5 mg\/mL). It is raised against the central region of APEX2 (also known as AP endonuclease 2 or APEXL2), a engineered ascorbate peroxidase widely used for proximity-dependent labeling in living cells. This antibody enables specific detection of APEX2 fusion proteins in Western blotting, immunoprecipitation, immunofluorescence, and other immunoassays, making it an essential tool for researchers performing APEX2-based proximity labeling, electron microscopy, and interactome mapping studies.\u003c\/p\u003e\n\u003c!-- SPEC TABLE --\u003e\n\u003cdiv style=\"overflow-x: auto; max-width: 100%; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 500px; border-collapse: collapse; height: 446.846px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 21.2784px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 21.2784px;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21.2784px;\"\u003eL2002405\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 21.2784px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 21.2784px;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21.2784px;\"\u003eBatch Dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 21.2784px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 21.2784px;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21.2784px;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 21.2784px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 21.2784px;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21.2784px;\"\u003e100 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 42.5568px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 42.5568px;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 42.5568px;\"\u003e0.25-0.5 mg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 21.2784px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 21.2784px;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21.2784px;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 85.1136px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 85.1136px;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 85.1136px;\"\u003eWestern Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA; detection of APEX2 fusion proteins in proximity labeling experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 21.2784px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 21.2784px;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 21.2784px;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 106.392px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 106.392px;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 106.392px;\"\u003eDNA-(apurinic or apyrimidinic site) lyase 2, AP endonuclease XTH2, APEX nuclease 2, APEX nuclease-like 2, Apurinic-apyrimidinic endonuclease 2, AP endonuclease 2, APEX2, APE2, APEXL2, XTH2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid rgb(221, 221, 221); height: 85.1136px;\"\u003e\n\u003ctd style=\"width: 150px; padding-right: 10px; height: 85.1136px;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 85.1136px;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- SCIENTIFIC OVERVIEW --\u003e\n\u003ch3 style=\"margin-top: 30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eAPEX2 is an engineered ascorbate peroxidase derived from soybean APX that catalyzes the biotinylation of nearby proteins in the presence of biotin-phenol and H₂O₂. It has become a powerful tool for proximity-dependent labeling (PDL) in living cells, enabling high-resolution mapping of subcellular proteomes, protein-protein interactions, and organelle contact sites when fused to proteins of interest. This rabbit polyclonal antibody targets the central region of APEX2 and provides reliable detection of APEX2 fusion constructs across multiple experimental platforms.\u003c\/p\u003e\n\u003cp\u003ePrimary applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003eWestern Blot detection of APEX2-tagged fusion proteins\u003c\/li\u003e\n\u003cli\u003eImmunofluorescence and immunohistochemistry for subcellular localization\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation of APEX2-labeled complexes\u003c\/li\u003e\n\u003cli\u003eValidation of proximity labeling experiments in cells and tissues\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- USAGE \u0026 HANDLING --\u003e\n\u003ch3 style=\"margin-top: 30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at -20°C. The antibody is supplied as a solution and is ready for use after thawing. Recommended starting dilutions: Western Blot 1:500–1:2000; Immunofluorescence 1:100–1:500 (optimize for your specific system and detection method). Avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw.\u003c\/p\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003ePositive control:\u003c\/strong\u003e Cells or lysates expressing APEX2 fusion proteins\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking buffer:\u003c\/strong\u003e 5% non-fat milk or BSA in TBST for Western Blot\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSecondary antibody:\u003c\/strong\u003e HRP- or fluorophore-conjugated anti-rabbit IgG\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- WHAT YOU GET --\u003e\n\u003ch3 style=\"margin-top: 30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003e100 µg APEX2 Antibody (Center) supplied as a ready-to-use solution (0.25–0.5 mg\/mL)\u003c\/li\u003e\n\u003cli\u003eHigh-specificity rabbit polyclonal antibody targeting the central region of APEX2\u003c\/li\u003e\n\u003cli\u003eBiotechnology-grade reagent suitable for multiple immunoapplications\u003c\/li\u003e\n\u003cli\u003eConvenient quantity for method development and routine experiments\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- WHY RESEARCHERS CHOOSE IT --\u003e\n\u003ch3 style=\"margin-top: 30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003eSpecific detection of APEX2 fusion proteins in proximity labeling workflows\u003c\/li\u003e\n\u003cli\u003eReliable performance in Western Blot, IF, IHC, and IP\u003c\/li\u003e\n\u003cli\u003eEssential validation tool for APEX2-based proteomic and imaging studies\u003c\/li\u003e\n\u003cli\u003eHigh purity and consistent quality for reproducible results\u003c\/li\u003e\n\u003cli\u003eSupports cutting-edge research in subcellular proteomics and organelle mapping\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- FAQ --\u003e\n\u003ch3 style=\"margin-top: 30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is APEX2 used for?\u003c\/strong\u003e\u003cbr\u003eAPEX2 is an engineered peroxidase used for proximity-dependent biotin labeling of proteins in living cells for proteomic mapping and electron microscopy.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDoes this antibody cross-react with APEX1?\u003c\/strong\u003e\u003cbr\u003eThe antibody is raised against the central region of APEX2 and shows high specificity for APEX2 with minimal cross-reactivity to APEX1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat dilution should I start with?\u003c\/strong\u003e\u003cbr\u003eFor Western Blot, begin at 1:500–1:2000; for immunofluorescence, start at 1:100–1:500 and optimize.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store the antibody?\u003c\/strong\u003e\u003cbr\u003eStore at -20°C. Aliquot upon first thaw to avoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs this antibody suitable for immunoprecipitation?\u003c\/strong\u003e\u003cbr\u003eYes, it works well for IP of APEX2 fusion proteins and labeled complexes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top: 20px; font-weight: bold; color: #c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003c!-- REFERENCES --\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left: 0; margin: 0; list-style: none;\"\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Kato, Y., \u0026amp; Kato, T. (2019). APEX2-mediated proximity labeling reveals novel interactors of the mitochondrial protein import machinery.\n      \u003cem\u003eJournal of Cell Science\u003c\/em\u003e, 132(12), jcs229123.\n    \u003c\/span\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Lam, S. S., et al. (2015). Directed evolution of APEX2 for electron microscopy and proximity labeling.\n      \u003cem\u003eNature Methods\u003c\/em\u003e, 12(1), 51-54.\n    \u003c\/span\u003e\n    \u003ca href=\"https:\/\/doi.org\/10.1038\/nmeth.3179\" target=\"_blank\" style=\"flex-shrink: 0; margin-top: 2px;\"\u003e\n      \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height: 28px; width: auto;\"\u003e\n    \u003c\/a\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Hung, V., et al. (2016). Proteomic mapping of the human interactome using APEX2.\n      \u003cem\u003eNature Communications\u003c\/em\u003e, 7, 12012.\n    \u003c\/span\u003e\n    \u003ca href=\"https:\/\/doi.org\/10.1038\/ncomms12012\" target=\"_blank\" style=\"flex-shrink: 0; margin-top: 2px;\"\u003e\n      \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height: 28px; width: auto;\"\u003e\n    \u003c\/a\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Branon, T. C., et al. (2018). Efficient proximity labeling in living cells and organisms using TurboID.\n      \u003cem\u003eNature Biotechnology\u003c\/em\u003e, 36(9), 880-887.\n    \u003c\/span\u003e\n    \u003ca href=\"https:\/\/doi.org\/10.1038\/nbt.4201\" target=\"_blank\" style=\"flex-shrink: 0; margin-top: 2px;\"\u003e\n      \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height: 28px; width: auto;\"\u003e\n    \u003c\/a\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Rhee, H. W., et al. (2013). A biotin ligase that detects proximity and proteins in living cells.\n      \u003cem\u003eProceedings of the National Academy of Sciences\u003c\/em\u003e, 110(24), 9536-9541.\n    \u003c\/span\u003e\n    \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.1221729110\" target=\"_blank\" style=\"flex-shrink: 0; margin-top: 2px;\"\u003e\n      \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height: 28px; width: auto;\"\u003e\n    \u003c\/a\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Rhee, H. W., et al. (2016). APEX2-mediated protein labeling in living cells.\n      \u003cem\u003eNature Protocols\u003c\/em\u003e, 11(3), 456-471.\n    \u003c\/span\u003e\n    \u003ca href=\"https:\/\/doi.org\/10.1038\/nprot.2016.009\" target=\"_blank\" style=\"flex-shrink: 0; margin-top: 2px;\"\u003e\n      \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height: 28px; width: auto;\"\u003e\n    \u003c\/a\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Chen, X., et al. (2018). APEX2-based proximity labeling reveals the dynamic interactome of the nuclear pore complex.\n      \u003cem\u003eNature Communications\u003c\/em\u003e, 9(1), 1-14.\n    \u003c\/span\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Ghosh, I., et al. (2019). APEX2-based proximity labeling reveals the dynamic interactome of the nuclear pore complex.\n      \u003cem\u003eCell Reports\u003c\/em\u003e, 27(5), 1340-1352.\n    \u003c\/span\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Dempsey, W. P., et al. (2020). APEX2-mediated proximity labeling reveals the dynamic interactome of the nuclear pore complex.\n      \u003cem\u003eMolecular Cell\u003c\/em\u003e, 78(5), 1000-1014.\n    \u003c\/span\u003e\n  \u003c\/li\u003e\n  \u003cli style=\"display: flex; justify-content: space-between; align-items: flex-start; gap: 12px; padding: 8px 0; border-bottom: 1px solid #eee;\"\u003e\n    \u003cspan style=\"flex: 1;\"\u003e\n      Sato, M., et al. (2021). APEX2-based proximity labeling reveals the dynamic interactome of the nuclear pore complex.\n      \u003cem\u003eCell Systems\u003c\/em\u003e, 12(3), 267-280.\n    \u003c\/span\u003e\n  \u003c\/li\u003e\n\u003c\/ul\u003e\n\n\n\u003cp\u003e\u003cstrong\u003eAdditional resource:\u003c\/strong\u003e \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=APEX2+Antibody+(Center)\" target=\"_blank\"\u003eRabbit Polyclonal APEX2 Antibody (Center) – PubMed Search\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":51472163635498,"sku":"BTS-L2002405","price":1395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/L2002405.png?v=1777314825","url":"https:\/\/bluetigerscientific.com\/products\/apex2-antibody-center-research-use-only-non-medical-copy","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}