{"product_id":"anti-v5-magnetic-beads","title":"Anti-V5 Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-V5 Magnetic Beads – Catalog #B2025667\u003c\/h2\u003e\n\u003cp\u003eAnti-V5 Magnetic Beads (Catalog #B2025667) are affinity purification reagents that combine a magnetic core with antibodies targeting the V5 epitope tag. V5 is a 14 amino acid peptide sequence derived from the paramyxovirus SV5, widely used in molecular and cell biology as a small, immunogenic affinity tag. These beads provide one-step immunomagnetic capture of V5-fusion proteins with minimal sample handling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025667\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunomagnetic affinity purification of V5-tagged proteins, co-immunoprecipitation (co-IP), protein complex isolation, rapid pull-down assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eV5-tag purification, anti-V5 antibody beads, V5 epitope affinity, magnetic immunoprecipitation, V5 protein isolation, V5 fusion protein purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe V5 epitope tag is a 14 amino acid peptide (GKPIPNPLLGLDST) recognized by widely used anti-V5 monoclonal antibodies. V5 is small enough to be appended to proteins without disrupting function, yet immunogenic enough for reliable detection and purification. It is commonly fused to the C-terminus of proteins of interest, enabling rapid identification, localization, and isolation from complex mixtures.\u003c\/p\u003e\n\u003cp\u003eMagnetic bead-based purification offers practical advantages: no centrifugation is required, separation is rapid (minutes), and protein complexes remain intact under mild binding and wash conditions. The anti-V5 coating on these beads provides specific recognition of the V5 tag with minimal cross-reactivity to untagged cellular proteins.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step affinity purification of V5-tagged recombinant proteins\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation of V5-fusion complexes from whole-cell lysates\u003c\/li\u003e\n\u003cli\u003eProtein interaction mapping (co-IP experiments)\u003c\/li\u003e\n\u003cli\u003eQualitative assessment of tag accessibility and protein expression\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eGently equilibrate the beads (do not vortex) and add to your sample in binding buffer. Incubate at 4°C or room temperature with gentle rotation to allow antibody-epitope interaction. Apply a magnet to pellet beads, remove the supernatant, and perform 3–5 washes with ice-cold wash buffer. Elute bound protein with low pH buffer, urea solution, or SDS-PAGE loading buffer depending on downstream use.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding buffer:\u003c\/strong\u003e PBS, TBS, or mild cell lysis buffer (e.g., 1% Triton X-100, 150 mM NaCl, 50 mM Tris pH 7.4).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Include 0.1% detergent (Triton X-100 or Tween-20) and 150–500 mM NaCl to reduce non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e 100 mM glycine–HCl (pH 2.5), 6 M urea, or 1% SDS; pH 2.5 elution is mildest for downstream applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 µL of anti-V5 magnetic beads, ready to use\u003c\/li\u003e\n\u003cli\u003eRapid, one-step affinity capture of V5-tagged proteins\u003c\/li\u003e\n\u003cli\u003eMagnetic format requiring no centrifugation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eV5 tag is widely recognized and immunogenic\u003c\/li\u003e\n\u003cli\u003eMagnetic beads eliminate centrifugation steps, reducing sample handling time\u003c\/li\u003e\n\u003cli\u003eWorks in a broad range of lysis and binding buffers\u003c\/li\u003e\n\u003cli\u003eCompatible with downstream mass spectrometry and protein assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the V5 epitope tag?\u003c\/strong\u003e\u003cbr\u003eThe V5 epitope is a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the paramyxovirus SV5 and recognized by widely used monoclonal antibodies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads at room temperature or must I use 4°C?\u003c\/strong\u003e\u003cbr\u003eRoom temperature incubation is acceptable and often faster; 4°C is recommended to minimize non-specific binding and protein degradation during extended incubations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the binding capacity?\u003c\/strong\u003e\u003cbr\u003eBinding capacity depends on protein size and concentration. Start with 25–50 µL of beads per 1–10 µg of V5-tagged target and optimize based on your recovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I perform ChIP with these beads?\u003c\/strong\u003e\u003cbr\u003eYes. V5-tagged chromatin proteins can be cross-linked and immunoprecipitated with these beads; include a cross-linking step (e.g., formaldehyde) before lysis and pull-down.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the shelf life?\u003c\/strong\u003e\u003cbr\u003eStore at –20°C; typical shelf life is 12–24 months. Avoid repeated freeze–thaw cycles; aliquot before freezing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZheng X, Pincus D. Serial Immunoprecipitation of 3xFLAG\/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins. \u003cem\u003eBio Protoc.\u003c\/em\u003e 2017;7(12).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.21769\/BioProtoc.2348\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKolodziej KE, Pourfarzad F, de Boer E, Krpic S, Grosveld F, Strouboulis J. Optimal use of tandem biotin and V5 tags in ChIP assays. \u003cem\u003eBMC Mol Biol.\u003c\/em\u003e 2009;10:6.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1186\/1471-2199-10-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eRanawakage DC, Takada T, Kamachi Y. HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions. \u003cem\u003eSci Rep.\u003c\/em\u003e 2019;9(1):6895.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/s41598-019-43319-y\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809016934698,"sku":"BTS-B2025667","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025667.png?v=1790859380","url":"https:\/\/bluetigerscientific.com\/products\/anti-v5-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}