{"product_id":"anti-mcherry-nanobody-magnetic-beads","title":"Anti-mCherry Nanobody Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-mCherry Nanobody Magnetic Beads – Catalog #B2025472\u003c\/h2\u003e\n\u003cp\u003eAnti-mCherry Nanobody Magnetic Beads (Catalog #B2025472) are affinity purification reagents designed for one-step capture and isolation of mCherry-tagged proteins. The beads combine a superparamagnetic core with nanobodies specific to the red fluorescent protein mCherry, enabling efficient immunomagnetic separation directly compatible with standard workflows.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025472\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e0.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunomagnetic affinity purification of mCherry-tagged proteins, protein complex isolation, rapid pull-down assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003emCherry-tag purification, nanobody affinity beads, magnetic immunoprecipitation, mCherry protein isolation, red fluorescent protein purification, nanobody magnetic particles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003emCherry is a bright monomeric red fluorescent protein widely used as an affinity epitope in recombinant protein work. Nanobodies are single-domain antibodies derived from camelids, offering compact size (~15 kDa), high thermal stability, and exceptional binding affinity. Anti-mCherry nanobodies recognize conformational epitopes on the mCherry protein body with Kd values in the nM range. When coupled to magnetic particles, they create a robust affinity matrix for immunomagnetic capture.\u003c\/p\u003e\n\u003cp\u003eCompared to conventional anti-mCherry antibodies, nanobodies offer superior resistance to denaturants and proteolysis, making them ideal for challenging purification conditions. The magnetic particles allow rapid, equipment-free separation: bind protein complexes in minutes, wash away contaminants, and elute the target.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step affinity purification of mCherry-fusion proteins from cell lysates\u003c\/li\u003e\n\u003cli\u003eProtein complex co-immunoprecipitation (co-IP)\u003c\/li\u003e\n\u003cli\u003eRapid pull-down assays and binding kinetics studies\u003c\/li\u003e\n\u003cli\u003eInteractome mapping and multi-protein isolation workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eEquilibrate the beads to room temperature before use. Resuspend gently (do not vortex) and add to your sample or lysis buffer. Incubate at 4°C or room temperature with gentle rotation (minutes to hours, depending on target concentration). Apply a magnet to pellet the beads, remove supernatant, wash 3–5 times with PBS or binding buffer, and elute with low pH or denaturing buffer.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Use physiological buffers (PBS, TBS) or mild lysis buffers; extreme pH or high salt may reduce binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Include detergent (0.1% Triton X-100 or Tween-20) in wash buffer to remove non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Low pH (pH 2–3, 0.1 M glycine–HCl), 6 M urea, or SDS (1%) will elute bound protein; choose based on downstream analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e0.1 mL of anti-mCherry nanobody magnetic beads, ready to use\u003c\/li\u003e\n\u003cli\u003eHigh-affinity, specific capture of mCherry-tagged proteins\u003c\/li\u003e\n\u003cli\u003eFast separation using a standard magnet\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eNanobodies provide higher affinity and stability than conventional antibodies\u003c\/li\u003e\n\u003cli\u003eMagnetic format enables hands-on, equipment-free purification\u003c\/li\u003e\n\u003cli\u003eRapid protocol suitable for time-sensitive work\u003c\/li\u003e\n\u003cli\u003eMinimal sample handling reduces loss of protein complexes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the Kd of anti-mCherry nanobodies?\u003c\/strong\u003e\u003cbr\u003eHigh-affinity anti-mCherry nanobodies typically bind with Kd values in the low nM range, though the exact value may vary by nanobody clone. Contact us for the lot-specific characterization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads with fluorescence detection?\u003c\/strong\u003e\u003cbr\u003eYes. The magnetic core does not interfere with fluorescence from mCherry or co-expressed tags; however, measure fluorescence before magnet application to avoid quenching by the iron oxide.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads suitable for native or denaturing conditions?\u003c\/strong\u003e\u003cbr\u003ePrimarily optimized for native conditions (physiological pH and ionic strength). Nanobodies tolerate moderate denaturants (e.g., 1% SDS) but are best used under conditions that preserve protein-protein interactions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much protein can the beads capture?\u003c\/strong\u003e\u003cbr\u003eBinding capacity depends on target size and nanobody density. Start with 10–50 µL of bead suspension per 1–5 µg of target; scale up or down based on recovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse the beads?\u003c\/strong\u003e\u003cbr\u003eBeads can be reused after thorough washing and regeneration, though repeated rounds may reduce binding efficiency. For most applications, fresh beads are recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I know if binding was successful?\u003c\/strong\u003e\u003cbr\u003ePerform a positive control by spiking your sample with a known mCherry-tagged protein, or measure absorbance at 587 nm (mCherry excitation wavelength) before and after binding.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCong ATQ, Witter TL, Schellenberg MJ. High-efficiency recombinant protein purification using mCherry and YFP nanobody affinity matrices. \u003cem\u003eProtein Sci.\u003c\/em\u003e 2022;31(9):e4383.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/pro.4383\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLiang H, Ma Z, Wang Z, Zhong P, Li R, Jiang H, Zong X, Zhong C, Liu X, Liu P, Liu J, Zhu H, Liu R, Ding Y. Structural Insights into the Binding of Red Fluorescent Protein mCherry-Specific Nanobodies. \u003cem\u003eInt J Mol Sci.\u003c\/em\u003e 2023;24(8).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3390\/ijms24086952\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWang Z, Li L, Hu R, Zhong P, Zhang Y, Cheng S, Jiang H, Liu R, Ding Y. Structural insights into the binding of nanobodies LaM2 and LaM4 to the red fluorescent protein mCherry. \u003cem\u003eProtein Sci.\u003c\/em\u003e 2021;30(11):2298-2309.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/pro.4194\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT, Rout MP. A robust pipeline for rapid production of versatile nanobody repertoires. \u003cem\u003eNat Methods.\u003c\/em\u003e 2014;11(12):1253-60.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/nmeth.3170\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809016574250,"sku":"BTS-B2025472","price":1205.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025472.png?v=1790859346","url":"https:\/\/bluetigerscientific.com\/products\/anti-mcherry-nanobody-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}