{"product_id":"anti-mbp-magnetic-beads","title":"Anti-MBP Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-MBP Magnetic Beads – Catalog #B2025665\u003c\/h2\u003e\n\u003cp\u003eAnti-MBP Magnetic Beads (Catalog #B2025665) are laboratory tools for rapid affinity purification of recombinant proteins fused to Maltose Binding Protein (MBP). The beads are coated with antibodies that recognize MBP, enabling one-step magnetic bead-based separation of MBP fusion proteins from complex protein mixtures. Supplied as 500 μL of suspension, they provide a convenient, fast alternative to column chromatography for protein purification workflows in molecular biology, structural biology, and biochemistry research.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025665\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget tag:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMaltose Binding Protein (MBP; 42 kDa from \u003cem\u003eE. coli\u003c\/em\u003e)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead properties:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic, used with magnetic separation racks\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification of MBP-tagged proteins, co-immunoprecipitation, protein-protein interaction studies, magnetic bead-based separation, solubility-enhanced protein isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-MBP beads, MBP affinity beads, maltose-binding protein purification, MBP magnetic beads, MBP-tagged proteins, MBP-fusion proteins, magnetic affinity purification, maltose binding protein isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eMaltose Binding Protein (MBP), from the bacterial maltose transport system of \u003cem\u003eEscherichia coli\u003c\/em\u003e, has become one of the most widely used protein fusion tags in structural and functional biology. At 42 kDa, MBP is larger than many other tags but offers unique advantages: it significantly improves the solubility and folding of fused partner proteins, is highly immunogenic (facilitating antibody production), and binds maltose and other oligosaccharides with useful affinity, allowing for alternative purification strategies if needed.\u003c\/p\u003e\n\u003cp\u003eAnti-MBP magnetic beads leverage the accessibility of MBP and the specificity of anti-MBP antibodies to capture and isolate MBP fusion proteins with minimal non-specific binding. They are particularly valuable when high-solubility protein production is required, or when expression systems rely on MBP as a primary solubility tag. The beads work well for co-immunoprecipitation experiments and rapid protein purification where chromatography is impractical.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step affinity purification of MBP-tagged recombinant proteins\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of protein-protein interaction partners\u003c\/li\u003e\n\u003cli\u003eIsolation of MBP fusion proteins from bacterial or insect cell lysates\u003c\/li\u003e\n\u003cli\u003ePull-down assays in structural and cell biology studies\u003c\/li\u003e\n\u003cli\u003eRapid sample preparation for biochemical assays and mass spectrometry\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eThese beads are supplied as a suspension. Gently mix before each use; avoid vortexing, which may damage the antibody coating. Add the beads directly to your lysate or protein mixture and incubate at room temperature or 4°C for 30–60 minutes to allow binding. Place the tube in a magnetic rack for 1–2 minutes to pellet the beads, then remove the supernatant and wash the beads 3–5 times with binding buffer or PBS.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding buffer:\u003c\/strong\u003e PBS, TBS, or your native lysis buffer works well. Avoid harsh detergents or organic solvents that may compromise the antibody coating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation time:\u003c\/strong\u003e 30–60 minutes is typical. Longer incubations at 4°C improve recovery while minimizing temperature-dependent protein degradation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Perform 3–5 washes with 10–15 bead volumes of binding buffer per wash to remove non-specifically bound proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute with low pH buffer (0.1 M glycine, pH 2–3), denaturing conditions (6 M urea, 8 M guanidinium chloride), or by competitive elution with free maltose or other MBP ligands.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a commercial magnetic rack designed for 1.5 or 2 mL tubes (e.g., DynaMag, Invitrogen).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 μL of anti-MBP magnetic beads in suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use beads for immediate affinity purification\u003c\/li\u003e\n\u003cli\u003eHigh-specificity antibodies against Maltose Binding Protein\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMBP is one of the most effective solubility-enhancing tags for recombinant protein expression\u003c\/li\u003e\n\u003cli\u003eMagnetic bead format is faster and more convenient than column chromatography\u003c\/li\u003e\n\u003cli\u003eHigh affinity and specificity of anti-MBP antibodies minimize non-specific binding\u003c\/li\u003e\n\u003cli\u003eWorks with MBP-tagged proteins from any expression system (bacterial, insect, yeast, mammalian)\u003c\/li\u003e\n\u003cli\u003eCompatible with standard co-immunoprecipitation and pull-down protocols\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I remove the MBP tag after purification?\u003c\/strong\u003e\u003cbr\u003eYes. MBP can be cleaved by proteases such as Factor Xa or TEV protease. If you need MBP-free protein, perform purification with these beads first, then cleave with your protease of choice and re-purify if needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWill the beads work if my MBP fusion is very large (e.g., \u0026gt;100 kDa)?\u003c\/strong\u003e\u003cbr\u003eYes. MBP-based purification works well regardless of fusion partner size. Very large fusion proteins may show slightly slower binding kinetics; consider extending the incubation time to 60–90 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use a standard benchtop magnet instead of a magnetic rack?\u003c\/strong\u003e\u003cbr\u003eA dedicated magnetic rack is recommended for consistent, rapid bead precipitation. Benchtop magnets may work but are generally less efficient.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the bead size?\u003c\/strong\u003e\u003cbr\u003eSpecific bead diameter is not provided in standard documentation. Request the COA\/TDS for lot-specific information on bead size and surface area.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for in vivo applications?\u003c\/strong\u003e\u003cbr\u003eNo. These beads are for in vitro research use only (RUO). Do not use in living systems, animals, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003evan den Noort M, de Boer M, Poolman B. Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein. \u003cem\u003eJ Mol Biol.\u003c\/em\u003e 2021;433(15):167036.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jmb.2021.167036\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHou S, Wang W, Hao T, Lei H. MS2-MBP-Based Affinity Purification of Nucleus- or Cytoplasm-Localized lncRNA-Protein Complexes Formed In Vivo. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2023;2666:231-245.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-3191-1_17\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLebendiker M, Danieli T. Purification of Proteins Fused to Maltose-Binding Protein. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2017;1485:257-273.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-6412-3_13\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGumpena R, Lountos GT, Waugh DS. MBP-binding DARPins facilitate the crystallization of an MBP fusion protein. \u003cem\u003eActa Crystallogr F Struct Biol Commun.\u003c\/em\u003e 2018;74(Pt 9):549-557.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1107\/S2053230X18009901\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805762646314,"sku":"BTS-B2025665","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025665.png?v=1790801865","url":"https:\/\/bluetigerscientific.com\/products\/anti-mbp-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}