{"product_id":"anti-ha-magnetic-beads","title":"Anti-HA Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-HA Magnetic Beads – Catalog #B2025663\u003c\/h2\u003e\n\u003cp\u003eAnti-HA Magnetic Beads (Catalog #B2025663) are laboratory tools for rapid affinity purification of HA-tagged recombinant proteins. The beads are coated with monoclonal antibodies that bind the HA epitope (nine amino acids from influenza hemagglutinin: YPYDVPDYA), enabling one-step magnetic bead-based separation of HA fusion proteins from complex protein mixtures. Supplied as 500 μL of suspension, they offer a fast, convenient alternative to column chromatography for protein purification, immunoprecipitation, and protein-protein interaction studies.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025663\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget epitope:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHA tag (YPYDVPDYA; hemagglutinin from influenza)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead properties:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic, used with magnetic separation racks\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification of HA-tagged proteins, co-immunoprecipitation, protein-protein interaction studies, immunofluorescence, protein isolation from cell lysates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-HA beads, HA tag purification, HA-tagged protein isolation, HA epitope beads, HA magnetic beads, hemagglutinin tag, magnetic affinity purification, HA-tag magnetic particles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe HA epitope (YPYDVPDYA) is a nine-amino-acid tag derived from the influenza virus hemagglutinin protein. It is one of the smallest and least disruptive epitope tags used in molecular biology, making it ideal for applications where protein size and function must be preserved. The HA tag is easily recognized by well-characterized monoclonal antibodies and has been validated in thousands of studies across cell biology, developmental biology, and biochemistry.\u003c\/p\u003e\n\u003cp\u003eAnti-HA magnetic beads use high-affinity monoclonal antibodies to capture HA-tagged proteins with specificity and efficiency. The beads facilitate rapid isolation of HA fusion proteins from cell lysates, making them particularly valuable for co-immunoprecipitation (co-IP) experiments, protein complex purification, and studies of transient or weak protein-protein interactions. Their small size and mild handling make them suitable for maintaining protein complex integrity during purification.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step affinity purification of HA-tagged proteins from lysates\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of protein-protein interaction partners\u003c\/li\u003e\n\u003cli\u003eIsolation of protein complexes while preserving native interactions\u003c\/li\u003e\n\u003cli\u003ePull-down assays in structural and cell biology\u003c\/li\u003e\n\u003cli\u003eRapid sample preparation for mass spectrometry\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eThese beads are supplied as a suspension. Gently mix before each use; avoid vortexing, which may damage the antibody coating. Add the beads directly to your lysate or protein mixture and incubate at 4°C for 30–60 minutes to allow binding, then place the tube in a magnetic rack for 1–2 minutes to pellet the beads. Remove the supernatant and wash the beads 3–5 times with binding buffer or PBS.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding buffer:\u003c\/strong\u003e PBS, TBS, or your native lysis buffer works well. Standard lysis buffers (with protease inhibitors if desired) are compatible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation:\u003c\/strong\u003e 30–60 minutes at 4°C is typical. Longer incubations improve recovery but may also increase co-precipitation of non-specific proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Perform 3–5 washes with 10–15 bead volumes of binding buffer per wash to remove non-specifically bound proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute with low pH buffer (0.1 M glycine, pH 2.8–3.0) or denaturing conditions (6 M urea, 8 M guanidinium chloride). Some applications tolerate gentler elution with hot 2% SDS.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a commercial magnetic rack suitable for 1.5 or 2 mL tubes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 μL of anti-HA magnetic beads in suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use beads for immediate co-immunoprecipitation and purification\u003c\/li\u003e\n\u003cli\u003eHigh-specificity monoclonal antibodies against the HA epitope\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHA is a small, well-tolerated epitope tag with minimal impact on protein folding and function\u003c\/li\u003e\n\u003cli\u003eMagnetic bead format enables rapid purification without column chromatography\u003c\/li\u003e\n\u003cli\u003eMonoclonal antibodies provide excellent specificity and low background\u003c\/li\u003e\n\u003cli\u003eWorks well for co-immunoprecipitation and protein complex isolation\u003c\/li\u003e\n\u003cli\u003eCompatible with downstream applications including mass spectrometry\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow specific is the anti-HA antibody?\u003c\/strong\u003e\u003cbr\u003eMonoclonal anti-HA antibodies are highly specific to the HA epitope (YPYDVPDYA) and show negligible cross-reactivity with other epitope tags or endogenous proteins in cell lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads with live cells or only with lysates?\u003c\/strong\u003e\u003cbr\u003eThese beads are designed for use with cell lysates and purified protein. For whole-cell immunofluorescence, use anti-HA antibodies without magnetic beads.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the recovery efficiency?\u003c\/strong\u003e\u003cbr\u003eRecovery depends on incubation time, antibody-epitope affinity, and bead concentration. Typical recoveries are 50–90% under optimized conditions. Request a COA\/TDS for lot-specific performance data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I scale up the protocol?\u003c\/strong\u003e\u003cbr\u003eYes. These beads scale linearly; simply use proportionally more beads and longer incubation times. For large-scale purifications (\u0026gt;10 mL lysate), consider contacting us for bulk quantities and optimization advice.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I remove the beads from my sample?\u003c\/strong\u003e\u003cbr\u003ePlace the tube in a magnetic rack for 1–2 minutes and carefully pipette off the supernatant. Beads will adhere to the tube wall. A second brief incubation in the magnet can help ensure complete removal.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGötzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. \u003cem\u003eNat Commun.\u003c\/em\u003e 2019;10(1):4403.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/s41467-019-12301-7\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLiu D, Liu C, Hu J, Hang L, Li X, Wei Y, Zhu H, Zhang Q, Wang X. Construction and evaluation of HA-epitope-tag introduction onto the VP1 structural protein of a novel HY12 enterovirus. \u003cem\u003eVirology.\u003c\/em\u003e 2018;525:106-116.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.virol.2018.09.010\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZhao X, Li G, Liang S. Several affinity tags commonly used in chromatographic purification. \u003cem\u003eJ Anal Methods Chem.\u003c\/em\u003e 2013;2013:581093.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1155\/2013\/581093\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759893802,"sku":"BTS-B2025663","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025663.png?v=1790801843","url":"https:\/\/bluetigerscientific.com\/products\/anti-ha-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}