{"product_id":"anti-gst-magnetic-beads","title":"Anti-GST Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-GST Magnetic Beads – Catalog #B2025649\u003c\/h2\u003e\n\u003cp\u003eAnti-GST Magnetic Beads (Catalog #B2025649) are laboratory tools for rapid affinity purification of GST-tagged recombinant proteins. The beads are coated with antibodies that recognize the GST epitope, enabling one-step magnetic bead-based separation of GST fusion proteins from complex protein mixtures. Supplied as 500 μL of suspension, they provide a convenient alternative to column chromatography for protein purification workflows in molecular biology, structural biology, and biochemistry research.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025649\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget epitope:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlutathione S-Transferase (GST)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead properties:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic, used with magnetic separation racks\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification of GST-tagged proteins, co-immunoprecipitation, protein-protein interaction studies, magnetic bead-based separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-GST beads, GST affinity beads, GST-tagged protein purification, GST magnetic beads, GST-binding beads, glutathione S-transferase purification, magnetic affinity purification, GST-tag isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlutathione S-Transferase (GST) is a ~26 kDa protein originally from \u003cem\u003eSchistosoma japonicum\u003c\/em\u003e that binds glutathione with extremely high affinity (Kd ~1 μM or lower). The GST tag has become one of the most popular protein fusion tags in molecular biology because it increases solubility, aids in protein folding, and provides a simple purification handle using glutathione-conjugated chromatography resins or, as with these beads, antibody-based magnetic separation.\u003c\/p\u003e\n\u003cp\u003eAnti-GST magnetic beads exploit the GST tag's accessibility and the high specificity of anti-GST antibodies to capture and isolate GST fusion proteins with minimal non-specific binding. They work particularly well for protein-protein interaction studies, where GST-tagged bait proteins are used to pull down interaction partners, and for rapid purification workflows where chromatography equipment is unavailable or inconvenient.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step affinity purification of GST-tagged recombinant proteins\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of protein-protein interaction partners\u003c\/li\u003e\n\u003cli\u003eEnrichment of GST-tagged proteins from cell lysates\u003c\/li\u003e\n\u003cli\u003ePull-down assays for studying protein interactions\u003c\/li\u003e\n\u003cli\u003eRapid protein isolation for biochemical assays and structural studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eThese beads are supplied as a suspension. Gently mix before each use; vortexing may damage the antibody coating. Add the beads directly to your lysate or protein mixture and incubate at room temperature or 4°C for 20–60 minutes to allow binding, then place the tube in a magnetic rack for 1–2 minutes to pellet the beads. Remove the supernatant and wash the beads 3–5 times with binding buffer or PBS.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding buffer:\u003c\/strong\u003e PBS, TBS, or your native lysis buffer works well. Avoid harsh detergents or organic solvents, which may disrupt the antibody coating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation time:\u003c\/strong\u003e 20–60 minutes is typical. Longer incubation may improve recovery but can increase non-specific binding if lysates are kept at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Perform 3–5 washes with 10–15 bead volumes of binding buffer per wash to minimize non-specific protein co-precipitation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute with low pH buffer (0.1 M glycine, pH 2–3), denaturing conditions (6 M urea, 8 M guanidinium chloride), or by competitive elution with free glutathione.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a commercial magnetic rack designed for 1.5 or 2 mL tubes (e.g., DynaMag, Invitrogen).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 μL of anti-GST magnetic beads in suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use beads for immediate affinity purification\u003c\/li\u003e\n\u003cli\u003eHigh-specificity antibodies against the GST tag\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eGST is one of the most widely used and well-characterized protein tags\u003c\/li\u003e\n\u003cli\u003eMagnetic bead format is faster than column chromatography\u003c\/li\u003e\n\u003cli\u003eHigh affinity and specificity of anti-GST antibodies minimizes non-specific binding\u003c\/li\u003e\n\u003cli\u003eWorks with virtually all GST-tagged proteins regardless of the fusion partner\u003c\/li\u003e\n\u003cli\u003eCompatible with standard co-immunoprecipitation and pull-down protocols\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I optimize binding conditions?\u003c\/strong\u003e\u003cbr\u003eTitrate the amount of beads added (typically 5–25 μL per mL of lysate), incubation time (20–60 minutes), and salt concentration to maximize recovery while minimizing non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use a standard benchtop magnet instead of a magnetic rack?\u003c\/strong\u003e\u003cbr\u003eA dedicated magnetic rack is recommended for consistent, rapid bead precipitation. Benchtop magnets may work but are less efficient and may increase handling time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the best way to elute my protein?\u003c\/strong\u003e\u003cbr\u003eLow pH (glycine, pH 2–3) works well for most applications but may denature some proteins. Try denaturing conditions (urea, guanidinium) or competitive elution with free glutathione if your protein is sensitive to pH extremes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWill the beads interfere with downstream assays?\u003c\/strong\u003e\u003cbr\u003eRemove beads completely by placing the tube in the magnet rack. Pipette off the supernatant carefully to avoid transferring any beads. A second, very brief incubation in the magnet can help settle residual beads.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I request technical support or lot documentation?\u003c\/strong\u003e\u003cbr\u003eYes. Contact us for COA (Certificate of Analysis), TDS (Technical Data Sheet), or protocol optimization assistance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchäfer F, Seip N, Maertens B, Block H, Kubicek J. Purification of GST-Tagged Proteins. \u003cem\u003eMethods Enzymol.\u003c\/em\u003e 2015;559:127-39.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/bs.mie.2014.11.005\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGnanasekaran P, Pappu HR. Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2023;2690:111-115.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-3327-4_10\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBlock H, Maertens B, Spriestersbach A, Kubicek J, Schäfer F. Proteolytic Affinity Tag Cleavage. \u003cem\u003eMethods Enzymol.\u003c\/em\u003e 2015;559:71-97.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/bs.mie.2014.11.009\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eScheich C, Sievert V, Büssow K. An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. \u003cem\u003eBMC Biotechnol.\u003c\/em\u003e 2003;3:12.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1186\/1472-6750-3-12\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759074602,"sku":"BTS-B2025649","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025649.png?v=1790801834","url":"https:\/\/bluetigerscientific.com\/products\/anti-gst-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}