{"product_id":"anti-gfp-nanobody-magnetic-beads","title":"Anti-GFP Nanobody Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-GFP Nanobody Magnetic Beads – Catalog #B2025464\u003c\/h2\u003e\n\u003cp\u003eAnti-GFP Nanobody Magnetic Beads (Catalog #B2025464) are laboratory tools for rapid affinity purification of GFP-tagged recombinant proteins. The beads are coated with nanobodies—small (~15 kDa), camelid-derived antibody fragments that recognize GFP with high specificity and affinity. Supplied as 0.1 mL in solution, they facilitate one-step, magnetic bead-based separation of GFP-tagged target proteins from complex protein mixtures, enabling efficient isolation of protein-protein complexes and study of protein interactions.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025464\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e0.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget epitope:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGFP and GFP variants (EGFP, mGFP, superfolder GFP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead properties:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic, used with magnetic separation racks\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification of GFP-tagged proteins, co-immunoprecipitation, protein-protein interaction studies, magnetic bead-based separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-GFP nanobody, GFP-binding nanobody, GFP affinity beads, GFP magnetic beads, GFP purification, GFP-tagged protein isolation, nanobody affinity chromatography, magnetic affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eNanobodies (variable domains of camelid heavy-chain antibodies) have emerged as powerful alternatives to conventional antibodies for protein purification and detection. They are smaller (~15 kDa vs. ~150 kDa for full antibodies), more thermodynamically stable, and often exhibit higher specificity and affinity for their epitopes. GFP-specific nanobodies bind with Kd values in the picomolar to nanomolar range, making them exceptionally efficient for capturing GFP-tagged proteins.\u003c\/p\u003e\n\u003cp\u003eGreen Fluorescent Protein (GFP) and its variants (EGFP, superfolder GFP, mGFP) are among the most widely used protein tags in research, with applications spanning cell biology, structural biology, and biochemistry. Anti-GFP nanobody magnetic beads combine the targeting specificity of nanobodies with the convenience of magnetic separation, enabling researchers to isolate GFP-tagged proteins from complex lysates in minutes without requiring chromatography equipment.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid one-step affinity purification of GFP-tagged recombinant proteins\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of protein-protein interaction partners\u003c\/li\u003e\n\u003cli\u003eEnrichment of GFP-tagged proteins from cell lysates\u003c\/li\u003e\n\u003cli\u003eRemoval of non-tagged contaminant proteins\u003c\/li\u003e\n\u003cli\u003eStructural biology workflows requiring high-purity GFP fusion proteins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eThese beads are supplied as a suspension. Gently mix before each use; vortexing may damage the nanobody coating. Add the beads directly to your lysate or protein mixture and incubate at 4°C for 10–30 minutes to allow binding, then place the tube in a magnetic rack for 1–2 minutes to pellet the beads. Remove the supernatant and wash the beads 3–5 times with binding buffer or PBS.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation:\u003c\/strong\u003e Room temperature or 4°C; optimize based on your target protein's stability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding buffer:\u003c\/strong\u003e PBS, TBS, or your native lysis buffer works well. Avoid harsh detergents or organic solvents, which may disrupt the nanobody coating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute with low pH buffer (0.1 M glycine, pH 2–3) or denaturing conditions (e.g., 6 M urea, 8 M guanidinium chloride). Mild conditions (imidazole competition) may also be effective for some applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a commercial magnetic rack designed for 1.5 or 2 mL tubes (e.g., DynaMag, Invitrogen).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e0.1 mL of anti-GFP nanobody magnetic beads in solution\u003c\/li\u003e\n\u003cli\u003eReady-to-use beads for immediate affinity purification\u003c\/li\u003e\n\u003cli\u003eHigh-affinity nanobody targeting GFP and common variants\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eNanobodies offer superior specificity and picomolar to nanomolar affinity\u003c\/li\u003e\n\u003cli\u003eMagnetic bead format eliminates the need for chromatography columns\u003c\/li\u003e\n\u003cli\u003eFast purification: typically 30 minutes from lysate to isolated protein\u003c\/li\u003e\n\u003cli\u003eCompatible with standard protocols for immunoprecipitation and affinity purification\u003c\/li\u003e\n\u003cli\u003eWorks efficiently with a wide range of GFP variants\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhich GFP variants does this recognize?\u003c\/strong\u003e\u003cbr\u003eThese nanobodies bind GFP and common engineered variants including EGFP, superfolder GFP (sfGFP), and mGFP. Cross-reactivity with less common variants (e.g., mCherry, mOrange) may vary; contact us for variant-specific information.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use a standard benchtop magnet instead of a magnetic rack?\u003c\/strong\u003e\u003cbr\u003eA dedicated magnetic rack is recommended for consistent, rapid bead precipitation. Benchtop magnets may work but are less efficient and may increase sample handling time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the bead size?\u003c\/strong\u003e\u003cbr\u003eSpecific bead diameter is not provided by the supplier. Request the COA\/TDS for lot-specific information on bead size and surface area.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in a flow-through mode (negative selection)?\u003c\/strong\u003e\u003cbr\u003eThese beads are optimized for positive affinity capture. Flow-through mode may not be practical because the beads will immediately bind GFP-tagged proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long can I store eluted protein?\u003c\/strong\u003e\u003cbr\u003eEluted protein stability depends on your buffer and storage conditions. For extended storage, consider dialyzing into a physiological buffer and storing at –20°C or –80°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKubala MH, Kovtun O, Alexandrov K, Collins BM. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. \u003cem\u003eProtein Sci.\u003c\/em\u003e 2010;19(12):2389-401.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/pro.519\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eStevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T. A nanobody-based strategy for rapid and scalable purification of human protein complexes. \u003cem\u003eNat Protoc.\u003c\/em\u003e 2024;19(1):127-158.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/s41596-023-00904-w\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMatsuda S, Aguilar G, Vigano MA, Affolter M. Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2022;2446:581-593.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-2075-5_30\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWeng D, Yang L, Xie Y. Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis. \u003cem\u003eProtein Expr Purif.\u003c\/em\u003e 2024;221:106501.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.pep.2024.106501\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805756584234,"sku":"BTS-B2025464","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025464.png?v=1790801825","url":"https:\/\/bluetigerscientific.com\/products\/anti-gfp-nanobody-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}