{"product_id":"anti-gfp-magnetic-beads","title":"Anti-GFP Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-GFP Magnetic Beads – Catalog #B2025655\u003c\/h2\u003e\n\u003cp\u003eAnti-GFP magnetic beads (Catalog #B2025655) are superparamagnetic particles covalently coated with monoclonal antibodies recognizing Green Fluorescent Protein (GFP) and GFP-based fluorescent proteins. Supplied as 500 µL of suspension, these beads provide rapid, one-step affinity capture and purification of GFP-tagged recombinant proteins directly from cell lysates, culture supernatants, or crude protein preparations. Magnetic separation eliminates centrifugation, reducing sample handling time and improving protein recovery while maintaining native protein conformation for downstream functional assays.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025655\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGreen Fluorescent Protein (GFP) and GFP-based tags\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAntibody type:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMonoclonal anti-GFP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGFP-tagged protein purification and isolation, immunoprecipitation of GFP-fusion proteins, co-immunoprecipitation of protein complexes, sample enrichment for proteomics and mass spectrometry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-GFP magnetic beads, GFP affinity beads, GFP-protein purification, magnetic GFP capture, anti-green fluorescent protein beads, GFP pulldown, GFP immunoprecipitation, GFP isolation beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGreen Fluorescent Protein (GFP) and its variants (EGFP, mCherry, mNeonGreen, etc.) are among the most widely used fluorescent tags in modern cell and molecular biology. GFP's ability to fold and fluoresce in living cells, combined with the availability of high-affinity antibodies and the ease of genetic fusion, has made it the standard tag for visualizing protein localization and dynamics. Anti-GFP magnetic beads extend this utility by enabling rapid, affinity-based isolation of GFP-tagged proteins from complex cellular environments.\u003c\/p\u003e\n\u003cp\u003eThe monoclonal anti-GFP antibody coating these beads binds the GFP chromophore and its immediate protein environment with high affinity and specificity, enabling selective capture of GFP-tagged proteins while leaving non-tagged proteins in solution. Because GFP fluorescence does not depend on antibody binding, proteins can be visualized even after bead capture, allowing real-time monitoring of purification and enabling fluorescence-based detection of eluted material.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid isolation of GFP-tagged recombinant proteins from cell lysates\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of protein complexes containing GFP-fusion components\u003c\/li\u003e\n\u003cli\u003eLive-cell protein tracking: capture GFP-tagged proteins directly from living cells\u003c\/li\u003e\n\u003cli\u003eSample enrichment and cleanup before mass spectrometry proteomics studies\u003c\/li\u003e\n\u003cli\u003eFunctional studies: isolate native GFP-protein complexes without denaturation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at −20°C. Thaw at room temperature and mix gently (avoid vortexing) before use. Incubate beads with sample (typically 10–50 µL of bead suspension per mL of lysate) at 4°C for 30 minutes to 2 hours with gentle rotation, or at room temperature for 15–30 minutes with mixing. Use a magnetic rack to immobilize beads, then carefully remove the supernatant. Wash beads 2–3 times in appropriate buffer (PBS, Tris-HCl, or HEPES, pH 7.0–8.0); add detergent (0.1% Triton X-100 or Tween-20) if non-specific binding occurs. Elute with acidic buffer (0.1 M glycine–HCl, pH 2.8), high-salt buffer (500 mM–1 M NaCl), or GFP-competitive peptide, depending on downstream application. Neutralize acidic eluates immediately.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead concentration:\u003c\/strong\u003e Use 10–50 µL per mL of lysate; optimize based on GFP-protein abundance in your system.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation conditions:\u003c\/strong\u003e 4°C is gentler for labile proteins; room temperature is faster if protein stability permits.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWash stringency:\u003c\/strong\u003e Include detergent in washes if non-specific co-precipitation is observed. Increase detergent concentration (up to 0.5%) for highly stringent washing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Acidic elution is fastest and most efficient; neutral elution is preferred if the target protein is sensitive to low pH or if downstream assays require native conformation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 µL of anti-GFP magnetic bead suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use immunoprecipitation and protein isolation reagent\u003c\/li\u003e\n\u003cli\u003eConvenient magnetic separation—no centrifugation required\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-affinity monoclonal antibody ensures specific capture of GFP-fusion proteins\u003c\/li\u003e\n\u003cli\u003eMagnetic separation is fast, gentle, and reduces sample loss compared to centrifugation\u003c\/li\u003e\n\u003cli\u003eCompatible with live cells, cell lysates, and crude preparations\u003c\/li\u003e\n\u003cli\u003eGFP fluorescence allows real-time monitoring and post-purification validation\u003c\/li\u003e\n\u003cli\u003eMinimal sample loss during purification, improving yield of target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo these beads work with mCherry, mNeonGreen, or other GFP variants?\u003c\/strong\u003e\u003cbr\u003eThe anti-GFP monoclonal typically recognizes canonical GFP (wtGFP, EGFP, sfGFP) with highest affinity. Cross-reactivity with other fluorescent proteins varies. Contact us to confirm compatibility with your specific tag.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for live-cell protein capture?\u003c\/strong\u003e\u003cbr\u003eYes, if cells remain viable during the incubation period. Use gentle, isotonic buffers and minimize incubation time (10–15 min at room temperature) to preserve cell viability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much protein can I expect to recover?\u003c\/strong\u003e\u003cbr\u003eRecovery depends on GFP-protein abundance, antibody binding kinetics, and buffer composition. Typically, 30–80% of input GFP-protein can be recovered with standard protocols. Optimize for your specific target.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I analyze eluted proteins by SDS-PAGE immediately after magnetic bead purification?\u003c\/strong\u003e\u003cbr\u003eYes. If you elute with acidic buffer, neutralize first with 1 M Tris–HCl, pH 8.0 (typically 1 volume of Tris per 10 volumes of eluate), then proceed to SDS-PAGE or other downstream applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the best elution method for maintaining protein activity?\u003c\/strong\u003e\u003cbr\u003eFor functional assays, use high-salt buffer (500 mM NaCl) or competitive GFP-peptide elution (10–100 µg\/mL) rather than acid elution, as these gentler methods better preserve protein conformation and activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKubala MH, Kovtun O, Alexandrov K, Collins BM. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. \u003cem\u003eProtein Sci.\u003c\/em\u003e 2010;19(12):2389-401.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/pro.519\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT, Rout MP. A robust pipeline for rapid production of versatile nanobody repertoires. \u003cem\u003eNat Methods.\u003c\/em\u003e 2014;11(12):1253-60.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/nmeth.3170\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVélez-Bermúdez IC, Salazar-Henao JE, Riera M, Caparros-Ruiz D, Schmidt W. Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads. \u003cem\u003eSTAR Protoc.\u003c\/em\u003e 2022;3(2):101449.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.xpro.2022.101449\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805764153642,"sku":"BTS-B2025655","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025655.png?v=1790801882","url":"https:\/\/bluetigerscientific.com\/products\/anti-gfp-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}