{"product_id":"anti-flag-magnetic-beads","title":"Anti-Flag Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-Flag Magnetic Beads – Catalog #B2025661\u003c\/h2\u003e\n\u003cp\u003eAnti-Flag magnetic beads (Catalog #B2025661) are superparamagnetic particles covalently coated with monoclonal antibodies recognizing the FLAG epitope. Supplied as 500 µL of suspension, they provide rapid, one-step affinity capture and purification of FLAG-tagged recombinant proteins directly from cell lysates, culture media, or crude protein preparations. The magnetic core enables fast bead separation with an external magnet, reducing sample handling time and improving protein recovery compared to traditional centrifugation-based methods.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025661\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget epitope:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eFLAG tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAntibody type:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMonoclonal anti-FLAG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eFLAG-tagged protein purification, immunoprecipitation, pull-down assays, protein complex isolation, sample enrichment for proteomics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-FLAG magnetic beads, FLAG tag purification beads, magnetic immunoprecipitation, FLAG-protein isolation, anti-FLAG affinity beads, magnetic FLAG capture, FLAG affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe FLAG epitope is one of the most widely used protein tags in modern molecular biology. Its small size (~916 Da for DYKDDDDK), availability of high-affinity monoclonal antibodies, and minimal impact on protein function make it ideal for rapid purification and detection of recombinant proteins. Anti-FLAG magnetic beads combine the specificity of monoclonal antibody recognition with the convenience of magnetic separation, enabling researchers to isolate FLAG-tagged proteins in minutes rather than hours.\u003c\/p\u003e\n\u003cp\u003eThe superparamagnetic iron oxide core at the bead center becomes magnetized only in the presence of an external field, allowing rapid immobilization and release without permanent magnetic properties that could interfere with downstream applications. This property also enables facile washing: beads are immobilized for seconds to minutes while buffers are exchanged, then released by removing the magnet.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step isolation of FLAG-tagged proteins from complex cell lysates\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of native protein complexes\u003c\/li\u003e\n\u003cli\u003eRapid purification of recombinant proteins during expression screening\u003c\/li\u003e\n\u003cli\u003eSample enrichment and cleanup before mass spectrometry\u003c\/li\u003e\n\u003cli\u003eHigh-throughput protein isolation workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at −20°C. Thaw at room temperature and mix gently (do not vortex) before use. Incubate beads with sample (typically 10–50 µL of bead suspension per mL of lysate) at 4°C for 30 minutes to 2 hours with gentle rotation, or at room temperature for 15–30 minutes with mixing. Place the tube on a magnetic rack to immobilize the beads, then carefully remove the supernatant. Wash beads 2–3 times in appropriate buffer (PBS, Tris-HCl, or HEPES, pH 7.0–8.0), using the magnetic rack between washes. Elute bound proteins with acidic buffer (e.g., 0.1 M glycine–HCl, pH 2.8) and immediately neutralize with Tris base or 1 M Tris–HCl, pH 8.0.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead volume:\u003c\/strong\u003e Start with 10–20 µL per mL of sample and adjust if needed based on target protein abundance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation time and temperature:\u003c\/strong\u003e 4°C with mixing is gentler and suitable for labile proteins; room temperature is faster if protein stability permits.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWash buffer additives:\u003c\/strong\u003e Include 0.05–0.1% Tween-20 or Triton X-100 in wash buffers to reduce non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Acidic elution preserves antibody–bead binding for potential reuse; neutral elution (e.g., high-salt buffer or competitive peptide elution) may be preferred if acidic pH denatures your target protein.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 µL of anti-FLAG magnetic bead suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use immunoprecipitation and purification reagent\u003c\/li\u003e\n\u003cli\u003eConvenient magnetic separation, no centrifugation needed\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-affinity monoclonal antibody ensures specific capture of FLAG-tagged proteins\u003c\/li\u003e\n\u003cli\u003eMagnetic separation is faster and gentler than centrifugation\u003c\/li\u003e\n\u003cli\u003eBroad compatibility with cell types, tissues, and expression systems\u003c\/li\u003e\n\u003cli\u003eMinimal sample loss during purification compared to traditional chromatography\u003c\/li\u003e\n\u003cli\u003eWidely used and well-established protocols in the literature\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long can I incubate the sample with beads?\u003c\/strong\u003e\u003cbr\u003eIncubation for 30 min to 2 hours at 4°C is typical. Longer incubation improves capture but may increase non-specific binding. Optimize incubation time and temperature for your target protein and buffer composition.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I perform multiple rounds of elution and reuse?\u003c\/strong\u003e\u003cbr\u003eBeads can be regenerated by washing with acidic then neutral buffer, but we recommend fresh beads for each new sample to avoid cross-contamination. If reusing beads, validate purity and efficiency first.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat downstream applications are compatible with eluted proteins?\u003c\/strong\u003e\u003cbr\u003eProteins eluted in acidic buffer must be neutralized immediately. Downstream applications (SDS-PAGE, mass spectrometry, functional assays) are generally compatible; confirm stability and activity of your target protein under elution conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much buffer do I need for washing?\u003c\/strong\u003e\u003cbr\u003eUse ~1 mL of wash buffer per 10–20 µL of bead slurry, performing 2–3 washes. Adjust based on non-specific binding observed in negative controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat if I see incomplete protein elution?\u003c\/strong\u003e\u003cbr\u003eIncrease incubation time in elution buffer or use a lower pH (down to pH 2.2). Alternatively, use competitive elution with FLAG peptide (DYKDDDDK, ~10 µg\/mL in PBS) for milder conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBeugelink JW, Sweep E, Janssen BJC, Snijder J, Pronker MF. Structural Basis for Recognition of the FLAG-tag by Anti-FLAG M2. \u003cem\u003eJ Mol Biol.\u003c\/em\u003e 2024;436(16):168649.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jmb.2024.168649\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVélez-Bermúdez IC, Salazar-Henao JE, Riera M, Caparros-Ruiz D, Schmidt W. Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads. \u003cem\u003eSTAR Protoc.\u003c\/em\u003e 2022;3(2):101449.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.xpro.2022.101449\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchotte L, Rombaut B, Thys B. A liquid phase affinity capture assay using magnetic beads to study protein-protein interaction: the poliovirus-nanobody example. \u003cem\u003eJ Vis Exp.\u003c\/em\u003e 2012;(63).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/3937\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805762580778,"sku":"BTS-B2025661","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025661.png?v=1790801864","url":"https:\/\/bluetigerscientific.com\/products\/anti-flag-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}