{"product_id":"anti-dykddddk-magnetic-beads","title":"Anti-DYKDDDDK Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAnti-DYKDDDDK Magnetic Beads – Catalog #B2026668\u003c\/h2\u003e\n\u003cp\u003eAnti-DYKDDDDK magnetic beads (Catalog #B2026668) are superparamagnetic particles (~10–100 nm iron oxide core) coated with monoclonal antibodies against the DYKDDDDK (FLAG) epitope. Supplied as 2 mL of suspension, they enable single-step affinity capture and purification of FLAG-tagged proteins from complex mixtures such as cell lysates, culture supernatants, or crude protein preparations. The magnetic properties allow rapid bead recovery using a simple magnet, eliminating the need for centrifugation and reducing sample loss.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026668\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBinding capacity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e50 kDa per 1 mL settled beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget epitope:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eDYKDDDDK (FLAG tag, MW ~916 Da)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAntibody type:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMonoclonal antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eFLAG-tagged protein purification, immunoprecipitation, pull-down assays, rapid protein isolation from cell lysates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnti-FLAG magnetic beads, DYKDDDDK antibody beads, FLAG tag purification, magnetic immunoprecipitation, FLAG affinity beads, anti-DYKDDDDK particles, FLAG-tag isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe DYKDDDDK epitope (also called the FLAG tag) is a short octapeptide sequence widely used as a protein-affinity handle in molecular and cell biology. It is recognized with high affinity by specific monoclonal antibodies (such as M1 and M2 variants) and enables rapid one-step affinity purification of recombinant proteins. Immobilization of anti-FLAG antibodies onto magnetic particles combines the selectivity of antibody recognition with the convenience of magnetic separation, eliminating tedious centrifugation steps and improving recovery of labile proteins.\u003c\/p\u003e\n\u003cp\u003eSuperparamagnetic beads containing iron oxide cores become magnetized only in the presence of an external magnetic field, making them ideal for rapid and reversible capture and release of protein–bead complexes. This property enables fast washing steps (seconds to minutes) and reduces non-specific interactions caused by prolonged incubation on a solid support.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step isolation of FLAG-tagged proteins from cell lysates\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation of protein complexes\u003c\/li\u003e\n\u003cli\u003eRapid purification of recombinant proteins in expression systems\u003c\/li\u003e\n\u003cli\u003eSample enrichment before mass spectrometry or other downstream analysis\u003c\/li\u003e\n\u003cli\u003eHigh-throughput screening and bioassay workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at −20°C. Thaw at room temperature and mix gently before use. For optimal binding, use appropriate lysis or binding buffers (typically PBS, Tris-HCl, or HEPES, pH 7.0–8.0). Incubate beads with sample at 4°C for 30 minutes to 2 hours (or at room temperature for 15–30 minutes) with gentle mixing. Use an external magnet to immobilize beads and carefully remove the supernatant, then wash beads thoroughly in binding buffer (typically 2–3 washes). Elute bound proteins with glycine–HCl (pH 2.8) or acidic buffer, followed by immediate neutralization.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead concentration:\u003c\/strong\u003e Use 10–50 µL of bead suspension per mL of sample, depending on target protein abundance and desired purification stringency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation time:\u003c\/strong\u003e Longer incubation (1–2 hours) improves capture efficiency; shorter incubation (15 min) reduces non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWash stringency:\u003c\/strong\u003e Add detergent (0.1% Triton X-100 or Tween-20) to wash buffers if non-specific co-precipitation is observed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Acidic elution (glycine–HCl, pH 2.8) recovers proteins while maintaining antibody–bead binding; proteins must be neutralized immediately after elution to avoid denaturation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of anti-DYKDDDDK magnetic bead suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use immunoprecipitation reagent for FLAG-tagged protein capture\u003c\/li\u003e\n\u003cli\u003eConvenient magnetic separation for rapid bead isolation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHighly specific monoclonal antibody against the DYKDDDDK epitope\u003c\/li\u003e\n\u003cli\u003eMagnetic separation eliminates centrifugation and reduces sample loss\u003c\/li\u003e\n\u003cli\u003eFast workflow (30 min to 2 hours total time)\u003c\/li\u003e\n\u003cli\u003eCompatible with downstream proteomics, structural biology, and cell biology applications\u003c\/li\u003e\n\u003cli\u003eWell-established protocols and wide use in research and diagnostic labs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the DYKDDDDK epitope?\u003c\/strong\u003e\u003cbr\u003eDYKDDDDK is an 8-amino-acid peptide sequence (also called the FLAG tag) recognized with high affinity by specific monoclonal antibodies. It is widely used in molecular biology to tag and purify recombinant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much sample can these beads handle?\u003c\/strong\u003e\u003cbr\u003eBinding capacity is approximately 50 kDa per mL of settled beads. For larger quantities, use proportionally more beads or contact us for bulk options.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for Western blotting?\u003c\/strong\u003e\u003cbr\u003eYes. After immunoprecipitation, wash the beads and either boil them directly in SDS-PAGE sample buffer or elute the protein first, then proceed with standard Western blot protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat buffer should I use?\u003c\/strong\u003e\u003cbr\u003eAny pH 7.0–8.0 buffer suitable for your application works well (PBS, Tris-HCl, HEPES). Avoid buffers containing azide, which can reduce bead performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eNot recommended. While beads can be regenerated with acidic elution followed by washing, we recommend fresh beads for each purification to ensure consistent results and avoid cross-contamination between samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBeugelink JW, Sweep E, Janssen BJC, Snijder J, Pronker MF. Structural Basis for Recognition of the FLAG-tag by Anti-FLAG M2. \u003cem\u003eJ Mol Biol.\u003c\/em\u003e 2024;436(16):168649.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jmb.2024.168649\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVélez-Bermúdez IC, Salazar-Henao JE, Riera M, Caparros-Ruiz D, Schmidt W. Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads. \u003cem\u003eSTAR Protoc.\u003c\/em\u003e 2022;3(2):101449.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.xpro.2022.101449\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchotte L, Rombaut B, Thys B. A liquid phase affinity capture assay using magnetic beads to study protein-protein interaction: the poliovirus-nanobody example. \u003cem\u003eJ Vis Exp.\u003c\/em\u003e 2012;(63).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/3937\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805760352554,"sku":"BTS-B2026668","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026668.png?v=1790801848","url":"https:\/\/bluetigerscientific.com\/products\/anti-dykddddk-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}