{"product_id":"amine-modified-polystyrene-latex-beads-9-0-9-9-um","title":"Amine-modified Polystyrene Latex Beads (9.0–9.9 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-modified Polystyrene Latex Beads (9.0–9.9 µm) – Catalog #B2025453\u003c\/h2\u003e\n\u003cp\u003eAmine-modified polystyrene latex beads (Catalog #B2025453) are uniform, monodisperse microspheres with a narrow size distribution (9.0–9.9 µm diameter) and reactive amine groups covalently bound to the polystyrene surface. Supplied as 5 mL of suspension, these particles are suitable for direct covalent labeling of biomolecules and are widely used in multiplexed assays, immunoprecipitation, and diagnostic applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025453\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e9.0–9.9 µm diameter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface modification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-functionalized (–NH₂ groups)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAntibody and protein conjugation, immunoassays, flow cytometry, multiplex bead arrays, protein purification, diagnostic assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-functionalized polystyrene beads, amine-modified microspheres, amino-modified latex beads, latex microspheres, polystyrene particles, protein-conjugated beads, carboxyl coupling beads, covalent protein coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePolystyrene latex beads are among the most versatile solid-phase matrices in molecular biology and diagnostics. Their spherical geometry, uniform size distribution, and ease of surface functionalization make them ideal platforms for building multiplexed assays and separation workflows. When coated with amine groups, the particles present reactive primary amino groups that can be directly coupled to carboxylated ligands (such as antibodies, proteins, or oligonucleotides) via standard EDC–NHS (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide–N-hydroxysuccinimide) chemistry or aldehyde-based cross-linking.\u003c\/p\u003e\n\u003cp\u003eThe 9.0–9.9 µm size range is large enough to be easily visualized by microscopy, handle by magnetic separation (when conjugated with magnetic components), and employ in flow cytometry, yet still small enough to provide high surface area for conjugation and adequate suspension stability. The narrow size distribution ensures consistent optical properties and reproducible assay performance across batches.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDirect covalent coupling of antibodies for multiplex immunoassays\u003c\/li\u003e\n\u003cli\u003eProtein A\/G-coated versions for antibody capture and purification\u003c\/li\u003e\n\u003cli\u003eLabeling with fluorescent or enzymatic markers for optical detection\u003c\/li\u003e\n\u003cli\u003eBead-based affinity purification and immunoprecipitation workflows\u003c\/li\u003e\n\u003cli\u003eFlow cytometric assays with multiplexing capability\u003c\/li\u003e\n\u003cli\u003eDiagnostic and clinical laboratory assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Mix gently before use (avoid vortexing, which may create aggregates). For optimal coupling, bring beads to room temperature and mix with ligands at the appropriate pH and molar ratios. Activation of amine groups by EDC–NHS cross-linker typically proceeds at room temperature for 30 minutes to 2 hours, followed by incubation with carboxyl-modified ligands. Allow adequate contact time (usually 2–4 hours) for coupling to reach saturation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003epH considerations:\u003c\/strong\u003e Optimal coupling pH is typically 4.5–6.0 for EDC–NHS chemistry. Extreme pH can protonate amines and reduce coupling efficiency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer choice:\u003c\/strong\u003e Use buffers free of primary amines (e.g., not Tris); Tris competes with bead amines for EDC and reduces coupling yield. MES (morpholinoethanesulfonic acid) or acetate buffers are preferred.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking unreacted amines:\u003c\/strong\u003e After coupling and washing, incubate beads in 1 M ethanolamine, pH 8.5, to block residual amine groups and reduce nonspecific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Always wash thoroughly between steps (coupling, blocking, and before use) using PBS or other appropriate buffers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL suspension of uniform amine-modified polystyrene latex beads (9.0–9.9 µm diameter)\u003c\/li\u003e\n\u003cli\u003eReactive amine groups ready for direct covalent conjugation\u003c\/li\u003e\n\u003cli\u003eNarrow size distribution for consistent assay performance\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eUniform, monodisperse particles ensure reproducible assay results\u003c\/li\u003e\n\u003cli\u003eReady-to-use amine surface simplifies antibody and protein conjugation\u003c\/li\u003e\n\u003cli\u003eLarge particle size (9–10 µm) is ideal for visualization and multiplexing in flow cytometry\u003c\/li\u003e\n\u003cli\u003eHigh purity and sterility support sensitive diagnostic applications\u003c\/li\u003e\n\u003cli\u003eWell-established protocols available in the literature for standard coupling chemistries\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I conjugate an antibody to these beads?\u003c\/strong\u003e\u003cbr\u003eUse EDC–NHS carbodiimide chemistry: activate the amine groups with EDC and NHS in MES buffer (pH 4.5–6.0) for 30 min, then incubate with your carboxyl-modified antibody for 2–4 hours. Wash and block with ethanolamine. See our Protein Conjugation Guide or contact us for detailed protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the typical binding capacity?\u003c\/strong\u003e\u003cbr\u003eCapacity depends on the size and purity of your ligand. Request the Certificate of Analysis or contact us for lot-specific conjugation data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these for magnetic separation?\u003c\/strong\u003e\u003cbr\u003eThese beads are not inherently magnetic. However, you can conjugate them with streptavidin and use magnetic streptavidin-coated beads, or purchase pre-made magnetic versions of amine-modified beads.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads compatible with my fluorescence instrument?\u003c\/strong\u003e\u003cbr\u003ePolystyrene has low autofluorescence and is compatible with most fluorophores. Confirm that your microscope or flow cytometer has filters appropriate for your chosen fluorescent label.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store the beads after coupling?\u003c\/strong\u003e\u003cbr\u003eStore conjugated beads at 2–8°C in PBS or other suitable buffer. Add 0.1% sodium azide as a preservative if storing for extended periods. Always avoid freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVignali DA. Multiplexed particle-based flow cytometric assays. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2000;243(1-2):243-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(00)00238-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. \u003cem\u003eExp Hematol.\u003c\/em\u003e 2002;30(11):1227-37.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0301-472x(02)00922-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGraham H, Chandler DJ, Dunbar SA. The genesis and evolution of bead-based multiplexing. \u003cem\u003eMethods.\u003c\/em\u003e 2019;158:2-11.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ymeth.2019.01.007\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLi T, Wang W, Cheng Y, Han W. Method for quantitative detection of FAM19A4 by flow cytometry using latex beads as solid carrier. \u003cem\u003eJ Biosci Bioeng.\u003c\/em\u003e 2018;125(3):359-364.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiosc.2017.10.008\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759828266,"sku":"BTS-B2025453","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025453.png?v=1790801841","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-9-0-9-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}