{"product_id":"amine-modified-polystyrene-latex-beads-8-0-8-9-um","title":"Amine-Modified Polystyrene Latex Beads (8.0–8.9 μm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-Modified Polystyrene Latex Beads (8.0–8.9 μm) – Catalog #B202636\u003c\/h2\u003e\n\u003cp\u003eAmine-Modified Polystyrene Latex Beads (Catalog #B202636) are precision-manufactured extra-large latex microspheres with covalently attached amino groups on the surface. The 8.0–8.9 μm diameter range represents the upper end of practical bead sizes for most biomedical applications, offering maximum surface area for protein loading, highest optical scatter intensity, and exceptional mechanical and physical stability. These beads are ideal for applications requiring high-capacity binding, easy visualization, and rugged handling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB202636\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e8.0–8.9 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface modification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine groups (NH₂)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension in phosphate-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge-scale protein purification, high-capacity immunoassays, immunomagnetic cell depletion and enrichment, enzyme immobilization, cell labeling and tracking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge amine polystyrene beads, 8 micron latex beads, extra-large microspheres, amine-modified particles, high-capacity binding beads, immunomagnetic beads, cell separation beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eBeads in the 8 μm size range represent a unique sweet spot for researchers who prioritize protein loading capacity and ease of visualization over absolute requirement for small particle size. At this diameter, each bead offers approximately 4-fold greater surface area than a 4 μm bead, enabling dramatically higher antibody or enzyme loading per unit mass of beads. The strong scatter signal means these beads are easily visualized by light microscopy (no fluorescent labeling required) and generate strong signals in flow cytometers without complex compensation.\u003c\/p\u003e\n\u003cp\u003eThe larger size also provides mechanical advantages: these beads are more robust during centrifugation, enzymatic digestion, or incubation in harsh buffers. They sediment rapidly and predictably, simplifying separation from solution. When conjugated with capture antibodies, they make excellent tools for immunomagnetic cell separation, where antibody-coated beads bind target cells, and magnetic nanoparticles or external magnets facilitate recovery.\u003c\/p\u003e\n\u003cp\u003eFor enzyme immobilization, the high surface area of large beads can support enzyme loadings in the range of 100–300 μg per mL of settled beads, creating highly active biocatalysts or biosensor surfaces. For affinity chromatography, these beads pack efficiently into large-volume columns while maintaining acceptable backpressure.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-capacity affinity purification and antibody\/protein capture\u003c\/li\u003e\n\u003cli\u003eLarge-scale enzyme immobilization for biocatalysis or biosensing\u003c\/li\u003e\n\u003cli\u003eImmunomagnetic cell enrichment or depletion workflows\u003c\/li\u003e\n\u003cli\u003eCell labeling, tracking, and microscopic analysis\u003c\/li\u003e\n\u003cli\u003eManufacturing of diagnostic or research reagent libraries\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C in the dark. Before use, gently invert the bottle 15–20 times to resuspend the beads evenly. The larger size and greater density mean these beads settle faster than smaller particles; resuspend immediately before use and work with fresh suspension. Do not vortex, as aggregation is more likely with larger particles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein coupling—high efficiency:\u003c\/strong\u003e The large surface area of 8 μm beads makes them ideal for high-efficiency protein coupling. EDC\/NHS chemistry typically yields \u0026gt;80% coupling efficiency even at high protein input. Glutaraldehyde coupling is also effective and can produce even higher cross-link densities.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-capacity binding studies:\u003c\/strong\u003e Use these beads for characterizing ligand–receptor interactions when high ligand density is important, or for kinetic studies where the large surface can accommodate multiple, simultaneous binding events.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunomagnetic separation:\u003c\/strong\u003e Conjugate with antibodies against cell-surface antigens; bind target cells in suspension; use magnetic nanoparticles (streptavidin-coated beads + biotin-labeled magnetic particles) or an external magnet to pull down bead-bound cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme immobilization:\u003c\/strong\u003e For biosensor or biocatalytic applications, use high enzyme loading (100–300 μg\/mL settled beads) to maximize activity per unit volume of material.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMicroscopy and imaging:\u003c\/strong\u003e These beads require no fluorescent dyes for visualization under brightfield microscopy. They are readily visible at 10× and higher magnification, simplifying tracking in cell-imaging experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of amine-modified polystyrene latex beads, 8.0–8.9 μm diameter\u003c\/li\u003e\n\u003cli\u003eMonodisperse, extremely uniform particles with maximum surface area\u003c\/li\u003e\n\u003cli\u003eReactive amine surface for robust protein immobilization\u003c\/li\u003e\n\u003cli\u003eExtra-large size for excellent visibility and mechanical stability\u003c\/li\u003e\n\u003cli\u003eReady-to-use suspension in phosphate-buffered saline\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eExceptional surface area enables maximum protein loading per bead\u003c\/li\u003e\n\u003cli\u003eExceptional optical scatter for easy flow cytometry and microscopy detection\u003c\/li\u003e\n\u003cli\u003eMechanical robustness ideal for aggressive assays and washing\u003c\/li\u003e\n\u003cli\u003eAmine surface allows efficient, high-yield protein coupling\u003c\/li\u003e\n\u003cli\u003ePerfect for immunomagnetic cell separation and enzyme immobilization\u003c\/li\u003e\n\u003cli\u003eRapid, predictable settling for straightforward handling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the maximum protein loading capacity of these beads?\u003c\/strong\u003e\u003cbr\u003eFor 8 μm beads, typical EDC\/NHS coupling yields 50–150 μg of protein per mL of settled beads. With glutaraldehyde, loading can reach 150–300 μg\/mL. Request the certificate of analysis for lot-specific binding capacity data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I see these beads under a light microscope without fluorescence?\u003c\/strong\u003e\u003cbr\u003eYes. The 8 μm size and strong scatter properties make these beads easily visible under brightfield light microscopy at 10× magnification and higher, without the need for fluorescent dyes or labels.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do these beads work with immunomagnetic cell separation?\u003c\/strong\u003e\u003cbr\u003eConjugate with antibodies targeting your cell of interest. Incubate with cell sample to allow binding. Then use biotin-labeled magnetic nanoparticles (streptavidin-coupled) or an external magnet to pull down bead-bound cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads suitable for packing into affinity columns?\u003c\/strong\u003e\u003cbr\u003eAbsolutely. The 8 μm size packs efficiently without excessive back-pressure. These columns achieve high binding capacity due to the large surface area of each bead and can handle substantial sample volumes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long do coupled beads remain stable?\u003c\/strong\u003e\u003cbr\u003eProtein-coupled beads stored at 4°C in blocking buffer (PBS + 1% BSA + 0.05% sodium azide) remain stable for 3–6 months. Avoid repeated freeze–thaw cycles and protect from light.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I dilute these beads, or should I use them at full concentration?\u003c\/strong\u003e\u003cbr\u003eYou can dilute as needed for your application, but the concentrated suspension allows you to work with less volume for high-capacity applications. For most assays, working at or near full concentration is economical.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVignali DA. Multiplexed particle-based flow cytometric assays. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2000;243(1-2):243-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(00)00238-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. \u003cem\u003eExp Hematol.\u003c\/em\u003e 2002;30(11):1227-37.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0301-472x(02)00922-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKumada Y, Miyamura Y, Tanibata R, Takahashi K, Ogasawara S, Gondaira F, Horiuchi JI. Design and site-directed immobilization of single-chain Fv antibody to polystyrene latex beads via material-binding peptides and application to latex turbidimetric assay. \u003cem\u003eJ Biosci Bioeng.\u003c\/em\u003e 2021;131(1):84-89.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiosc.2020.08.014\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKrishnan VV, Selvan SR, Parameswaran N, Venkateswaran N, Luciw PA, Venkateswaran KS. Proteomic profiles by multiplex microsphere suspension array. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2018;461:1-14.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jim.2018.07.002\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805760647466,"sku":"BTS-B202636","price":705.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B202636.png?v=1790801850","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-8-0-8-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}