{"product_id":"amine-modified-polystyrene-latex-beads-6-0-6-9-um","title":"Amine-Modified Polystyrene Latex Beads (6.0–6.9 μm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-Modified Polystyrene Latex Beads (6.0–6.9 μm) – Catalog #B2025693\u003c\/h2\u003e\n\u003cp\u003eAmine-Modified Polystyrene Latex Beads (Catalog #B2025693) are large, uniform latex microspheres with amino groups covalently coupled to the surface. The 6.0–6.9 μm diameter range strikes an ideal balance for applications where robust particle handling, excellent visibility under light microscopy, and high protein binding capacity are all required. These beads generate strong optical scatter, making them excellent for flow cytometry, and their larger size makes them mechanically stable during washing and manipulation.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025693\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6.0–6.9 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface modification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine groups (NH₂)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension in phosphate-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunoassay beads, cell labeling, immunomagnetic separation, microscopical applications, affinity chromatography, solid-phase enzyme assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge amine polystyrene beads, 6 micron latex beads, amine microspheres, large-particle beads, protein-coupling beads, immunomagnetic beads, microscopy beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eLarger latex beads in the 6–7 μm size range have several advantages for bioassays and cell biology. Their large surface area provides high protein binding capacity, making them excellent for antibody immobilization, enzyme coupling, and ligand-binding studies. The strong optical scatter they produce simplifies detection by light microscopy or flow cytometry, reducing the need for internal fluorescent dyes.\u003c\/p\u003e\n\u003cp\u003eIn multiplexed assays, larger beads separate more easily from smaller beads and biomolecules in the sample, reducing cross-talk and improving assay specificity. They are also mechanically robust—less prone to damage during magnetic separation, centrifugation, or mixing—and settle predictably, making them ideal for high-throughput liquid handling.\u003c\/p\u003e\n\u003cp\u003eAmine-modified beads at this size are commonly used to prepare affinity chromatography columns for protein purification, to immobilize enzymes for bioanalysis or synthetic chemistry applications, and to develop immunoassays and immunomagnetic cell-separation methods.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-capacity affinity chromatography (protein purification, antibody capture)\u003c\/li\u003e\n\u003cli\u003eEnzyme immobilization for biosensors and biocatalysis\u003c\/li\u003e\n\u003cli\u003eLarge-scale immunoassay platforms and diagnostic reagent development\u003c\/li\u003e\n\u003cli\u003eImmunomagnetic cell separation (negative selection, positive selection)\u003c\/li\u003e\n\u003cli\u003eFluorescence microscopy and cell-imaging applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C in the dark. The beads settle readily; before use, gently invert the bottle or stir slowly to resuspend evenly. These beads are robust and tolerate more vigorous handling than smaller beads, but do not vortex or sonicate to avoid aggregation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling to antibodies or enzymes:\u003c\/strong\u003e Amine coupling via EDC\/NHS or glutaraldehyde works efficiently at this bead size. The large surface area often permits higher protein loading (μg per mL of beads) than smaller particles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColumn preparation:\u003c\/strong\u003e For affinity chromatography, these beads can be packed into spin columns or larger column formats. Pack at appropriate flow rates to avoid bead aggregation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Although non-magnetic by themselves, these beads can be used with anti-bead magnetic capture reagents or functionalized with streptavidin for biotin-labeled magnetic nanoparticle-mediated separation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMicroscopy:\u003c\/strong\u003e The 6–7 μm size is easily visible by light microscopy without added fluorescence labels. For fluorescence microscopy, these beads can be internally dyed or conjugated to fluorescent proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCentrifugation and washing:\u003c\/strong\u003e Use moderate centrifugation (1,000–2,000 × g) to pellet these beads without damage. Gentle resuspension in wash buffers prevents aggregation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of amine-modified polystyrene latex beads, 6.0–6.9 μm diameter\u003c\/li\u003e\n\u003cli\u003eMonodisperse, size-uniform particles with high surface area\u003c\/li\u003e\n\u003cli\u003eReactive amine surface suitable for robust protein immobilization\u003c\/li\u003e\n\u003cli\u003eReady-to-use suspension in phosphate-buffered saline\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge size provides excellent visibility and strong optical scatter\u003c\/li\u003e\n\u003cli\u003eHigh surface area enables substantial protein loading per bead\u003c\/li\u003e\n\u003cli\u003eMechanically robust, ideal for aggressive assay conditions\u003c\/li\u003e\n\u003cli\u003eAmine surface allows straightforward, efficient protein coupling\u003c\/li\u003e\n\u003cli\u003eCompatible with immunomagnetic, chromatographic, and microscopy workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the binding capacity of these beads?\u003c\/strong\u003e\u003cbr\u003eBinding capacity depends on protein size and coupling efficiency. Larger beads typically bind 50–200 ng of protein per microliter of settled beads. Request lot-specific data from the certificate of analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these suitable for cell-labeling experiments?\u003c\/strong\u003e\u003cbr\u003eYes. Their 6 μm size is large enough to visualize by light microscopy and small enough for many cell-interaction studies. Conjugate with cell-specific antibodies or fluorescent proteins for cell tracking or sorting.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in an affinity column?\u003c\/strong\u003e\u003cbr\u003eAbsolutely. Pack them into a spin column or larger format column; couple with ligand-binding antibodies or other capture molecules to create custom affinity purification columns.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent bead aggregation?\u003c\/strong\u003e\u003cbr\u003eStore in cool, dark conditions; invert gently before use (do not vortex). After protein coupling, resuspend in blocking buffer containing BSA and sodium azide to stabilize the conjugate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat if I need magnetic separation?\u003c\/strong\u003e\u003cbr\u003eThese polystyrene beads are non-magnetic. For magnetic separation, either look for magnetic amine latex bead equivalents, or couple these beads to streptavidin and use biotin-labeled magnetic nanoparticles for pull-down.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much protein can I couple in a single batch?\u003c\/strong\u003e\u003cbr\u003eUsing EDC\/NHS chemistry, typically 20–100 μg of protein per mL of settled beads. Run a small-scale pilot coupling to optimize for your specific protein and coupling conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVignali DA. Multiplexed particle-based flow cytometric assays. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2000;243(1-2):243-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(00)00238-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. \u003cem\u003eExp Hematol.\u003c\/em\u003e 2002;30(11):1227-37.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0301-472x(02)00922-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLi T, Wang W, Cheng Y, Han W. Method for quantitative detection of FAM19A4 by flow cytometry using latex beads as solid carrier. \u003cem\u003eJ Biosci Bioeng.\u003c\/em\u003e 2018;125(3):359-364.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiosc.2017.10.008\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKrishnan VV, Selvan SR, Parameswaran N, Venkateswaran N, Luciw PA, Venkateswaran KS. Proteomic profiles by multiplex microsphere suspension array. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2018;461:1-14.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jim.2018.07.002\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759697194,"sku":"BTS-B2025693","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025693.png?v=1790801839","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-6-0-6-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}