{"product_id":"amine-modified-polystyrene-latex-beads-6-0-6-9-um-5-percent-w-v","title":"Amine-Modified Polystyrene Latex Beads (6.0–6.9 μm, 5% w\/v)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-Modified Polystyrene Latex Beads (6.0–6.9 μm, 5% w\/v) – Catalog #B2025743\u003c\/h2\u003e\n\u003cp\u003eAmine-Modified Polystyrene Latex Beads (Catalog #B2025743) are large latex microspheres (6.0–6.9 μm diameter) with amino groups attached to the surface, supplied at high concentration (5% w\/v suspension). This concentrated formulation delivers approximately 50 mg of beads per mL, offering significant cost and space advantages for large-scale applications. The larger particle size combines excellent optical properties with high surface area and robust mechanical stability.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025743\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL (5% w\/v concentration)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6.0–6.9 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface modification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine groups (NH₂)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5% w\/v (approximately 50 mg\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-concentration suspension in phosphate-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge-scale affinity column preparation, high-volume antibody coupling, protein purification, enzyme immobilization, multiplexed immunoassay development\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-concentration latex beads, concentrated amine beads, 5% w\/v beads, large amine microspheres, protein coupling, affinity chromatography beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe 5% w\/v formulation is a concentrated suspension designed for applications where large quantities of bead material are needed. This concentration (approximately 50 mg of solid beads per mL) reduces the volume of storage space required and provides economic advantages for high-throughput, large-volume projects.\u003c\/p\u003e\n\u003cp\u003eThe 6.0–6.9 μm bead diameter offers several advantages: high surface area for protein loading, excellent optical scatter for flow cytometry and microscopy, mechanical robustness during washing and manipulation, and compatibility with standard chromatography techniques. Amine-modified beads readily couple to proteins via EDC\/NHS, glutaraldehyde, or other standard cross-linking chemistries.\u003c\/p\u003e\n\u003cp\u003eHigh-concentration suspensions can be diluted to lower working concentrations as needed, allowing researchers to use the same formulation for multiple applications and scales of experimentation. This concentrated form is particularly suited to in-house manufacturing of affinity columns, large-scale antibody conjugation, or development workflows where material is consumed quickly.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePreparation of large affinity chromatography columns for protein purification\u003c\/li\u003e\n\u003cli\u003eBulk-scale antibody and enzyme coupling for assay development\u003c\/li\u003e\n\u003cli\u003eImmunomagnetic cell separation (with streptavidin\/biotin-magnetic nanoparticle methodology)\u003c\/li\u003e\n\u003cli\u003eManufacturing of custom diagnostic or research reagents\u003c\/li\u003e\n\u003cli\u003eEnzyme immobilization for biosensor or biocatalysis applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eBefore use, gently invert the bottle 20–30 times to achieve uniform resuspension of the high-concentration suspension. Do not vortex or sonicate. Dispense carefully to avoid splashing. The high bead concentration means rapid settling; work with freshly resuspended suspension or stir periodically during use.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eDilution:\u003c\/strong\u003e For most applications, dilute the 5% w\/v stock to 1–2% w\/v working suspension in appropriate buffer before use. This improves handling and reduces background in some assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLarge-scale coupling:\u003c\/strong\u003e The concentrated format is ideal for batch conjugation. Use standard EDC\/NHS or glutaraldehyde protocols, scaling from published small-volume methods (multiply by the appropriate factor).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColumn packing:\u003c\/strong\u003e For affinity chromatography, this concentrated suspension can be efficiently packed into columns of varying sizes without requiring large fluid volumes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of coupled beads:\u003c\/strong\u003e After protein immobilization, store in appropriate blocking buffer containing BSA (1%), PBS, and sodium azide (0.05%) to maintain stability over weeks to months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParticle settling:\u003c\/strong\u003e Due to the high concentration, beads settle rapidly. Resuspend by gentle inversion immediately before each use to ensure uniform particle distribution.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of high-concentration (5% w\/v) amine-modified polystyrene latex bead suspension\u003c\/li\u003e\n\u003cli\u003eLarge 6.0–6.9 μm diameter particles with reactive amine surface\u003c\/li\u003e\n\u003cli\u003eApproximately 250 mg of total bead material in the supplied volume\u003c\/li\u003e\n\u003cli\u003eIdeal for large-scale protein coupling and affinity column preparation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh bead concentration reduces storage space and cost per unit of material\u003c\/li\u003e\n\u003cli\u003eLarge particle size provides excellent visibility and optical scatter\u003c\/li\u003e\n\u003cli\u003eAmine surface enables efficient, straightforward protein conjugation\u003c\/li\u003e\n\u003cli\u003eRobust, durable beads suitable for aggressive assay conditions and washing\u003c\/li\u003e\n\u003cli\u003eIdeal for scaling up immunoassay development and manufacturing workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I dilute the 5% w\/v suspension for use?\u003c\/strong\u003e\u003cbr\u003eFor most immunoassays, dilute 1–2 fold in phosphate-buffered saline or your assay buffer. Gently invert to mix; do not vortex to avoid aggregation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the total amount of bead material in this 5 mL vial?\u003c\/strong\u003e\u003cbr\u003eThe 5% w\/v suspension contains approximately 250 mg of polystyrene bead solids in the 5 mL volume. This corresponds to roughly 5–10 million particles per microliter, depending on particle density.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I aliquot the concentrated suspension for long-term storage?\u003c\/strong\u003e\u003cbr\u003eYes. Aliquot into smaller tubes (e.g., 1 mL per tube) and store at 2–8°C. Gently thaw and resuspend by inversion before use; avoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the optimal dilution for flow cytometry?\u003c\/strong\u003e\u003cbr\u003eFor flow cytometry, use 0.5–1% w\/v to achieve 10,000–50,000 bead events per sample. Optimize based on your instrument and assay requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much protein can I couple to this concentrated bead preparation?\u003c\/strong\u003e\u003cbr\u003eUsing standard EDC\/NHS chemistry, expect 20–100 μg of protein per mL of settled beads (50 mg in 1 mL at 5% w\/v). Run pilot experiments to optimize coupling efficiency for your protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this suspension directly in my assay, or must I dilute?\u003c\/strong\u003e\u003cbr\u003eThe concentration is suitable for many applications directly, but bead settling is rapid at 5% w\/v. For most workflows, diluting to 1–2% w\/v improves handling and reduces settling-related issues.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVignali DA. Multiplexed particle-based flow cytometric assays. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2000;243(1-2):243-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(00)00238-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. \u003cem\u003eExp Hematol.\u003c\/em\u003e 2002;30(11):1227-37.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0301-472x(02)00922-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eAhsan H. Monoplex and multiplex immunoassays: approval, advancements, and alternatives. \u003cem\u003eComp Clin Path.\u003c\/em\u003e 2022;31(2):333-345.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s00580-021-03302-4\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLi T, Wang W, Cheng Y, Han W. Method for quantitative detection of FAM19A4 by flow cytometry using latex beads as solid carrier. \u003cem\u003eJ Biosci Bioeng.\u003c\/em\u003e 2018;125(3):359-364.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiosc.2017.10.008\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805760057642,"sku":"BTS-B2025743","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025743.png?v=1790801844","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-6-0-6-9-um-5-percent-w-v","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}