{"product_id":"amine-modified-polystyrene-latex-beads-4-0-4-9-um","title":"Amine-Modified Polystyrene Latex Beads (4.0–4.9 μm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-Modified Polystyrene Latex Beads (4.0–4.9 μm) – Catalog #B202642\u003c\/h2\u003e\n\u003cp\u003eAmine-Modified Polystyrene Latex Beads (Catalog #B202642) are precision-engineered monodisperse microspheres with covalently attached amino groups on the surface. The 4.0–4.9 μm diameter range sits at the optimal size for many flow cytometric and immunological applications, combining excellent scatter signal with high surface-to-volume ratio and ease of handling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB202642\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4.0–4.9 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface modification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine groups (NH₂)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension in phosphate-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMultiplexed flow cytometric assays, antibody immobilization, cell sorting, sandwich immunoassays, protein capture, biomarker detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-modified polystyrene beads, amine latex microspheres, 4 micron beads, amine-functionalized particles, protein coupling beads, immunoassay beads, flow cytometry microspheres\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe 4.0–4.9 μm bead size has emerged as the gold standard for modern flow cytometric immunoassays because it provides optimal scatter signal intensity, sufficient surface area for antibody loading, and compatibility with standard flow cytometer optical systems. These beads are particularly suited to Luminex xMAP (multiplex assay platform) and similar multiplexed assays where beads of different sizes are used to create a multidimensional detection platform.\u003c\/p\u003e\n\u003cp\u003eAmine-modified beads conjugated with capture antibodies can form the basis of sensitive sandwich immunoassays in solution, in which detection antibodies labeled with fluorescent molecules bind to captured antigen on the bead surface. The bead format allows dramatic increases in assay throughput compared to plate-based formats because multiple colored beads can be read in parallel, and sample preparation is often simplified.\u003c\/p\u003e\n\u003cp\u003eThe mid-range particle size also makes these beads suitable for cell sorting and magnetic depletion strategies when conjugated with antibodies against cell-surface antigens.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMultiplexed Luminex-style cytokine and biomarker assays\u003c\/li\u003e\n\u003cli\u003eSandwich immunoassays for growth factors, hormones, and therapeutic proteins\u003c\/li\u003e\n\u003cli\u003eMagnetic bead-based cell isolation and immunomagnetic depletion\u003c\/li\u003e\n\u003cli\u003eAffinity chromatography columns for protein purification\u003c\/li\u003e\n\u003cli\u003eDevelopment of custom diagnostic or research immunological reagents\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C in the original suspension buffer, protected from light. The beads settle gradually; invert the bottle 10–20 times before use to achieve even resuspension. Do not vortex, sonicate, or shake vigorously.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein coupling protocols:\u003c\/strong\u003e EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) combined with NHS (N-hydroxysuccinimide) is the most common amine-to-carboxyl coupling strategy. Alternatively, glutaraldehyde cross-linking or direct streptavidin\/biotin coupling can be used.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimization:\u003c\/strong\u003e Coupling efficiency depends on pH, protein concentration, and cross-linker ratios. Always run small-scale optimization experiments before large-scale conjugation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMultiplexing:\u003c\/strong\u003e If using these beads in a multiplexed assay with other size-matched beads, confirm that your flow cytometer can reliably distinguish bead populations by forward\/side scatter or by internal fluorescent dyes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay format:\u003c\/strong\u003e For sandwich immunoassays, use capture antibodies at high surface density (maximizing binding) and detection antibodies labeled with R-phycoerythrin (PE), allophycocyanin (APC), or other flow cytometry-compatible fluorophores.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of conjugates:\u003c\/strong\u003e After coupling, store bead-antibody conjugates at 4°C in blocking buffer (typically PBS with BSA and sodium azide) at the concentration provided by your coupling kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of amine-modified polystyrene latex beads, 4.0–4.9 μm diameter\u003c\/li\u003e\n\u003cli\u003eHighly uniform, monodisperse particle population\u003c\/li\u003e\n\u003cli\u003eSurface amine groups ready for protein immobilization\u003c\/li\u003e\n\u003cli\u003eConvenient ready-to-use suspension format\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOptimal size for flow cytometry sensitivity and resolution\u003c\/li\u003e\n\u003cli\u003eAmine surface enables rapid, efficient protein conjugation\u003c\/li\u003e\n\u003cli\u003eIndustry-standard size for Luminex and multiplex platforms\u003c\/li\u003e\n\u003cli\u003eExcellent scatter properties for reliable bead detection\u003c\/li\u003e\n\u003cli\u003eExtensive published protocols supporting bead conjugation and multiplexing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow many proteins can I attach per bead?\u003c\/strong\u003e\u003cbr\u003eProtein loading density depends on protein size and coupling chemistry. Typical loading ranges from 10 to 100 ng of protein per microliter of beads. Request the TDS or COA for lot-specific capacity data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the optical signature of these beads in flow cytometry?\u003c\/strong\u003e\u003cbr\u003eThese beads display intermediate forward scatter (FSC) and side scatter (SSC) compared to smaller and larger latex beads, making them ideal for multiplexing without overlap in the FSC\/SSC gate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan these beads be used in immunomagnetic assays?\u003c\/strong\u003e\u003cbr\u003eNot directly, since these are non-magnetic polystyrene beads. To add magnetic properties, couple these beads with streptavidin and use biotin-labeled magnetic nanoparticles, or purchase the magnetic amine bead equivalent.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow stable are protein-conjugated beads in long-term storage?\u003c\/strong\u003e\u003cbr\u003eProtein-conjugated beads remain stable for several months at 4°C in appropriate blocking buffer. Avoid repeated freeze–thaw cycles and store in the dark to minimize protein degradation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use different detection methods (ELISA-like, turbidimetry) with these beads?\u003c\/strong\u003e\u003cbr\u003eYes. Beyond flow cytometry, these beads work well in ELISA-adapted formats and in latex agglutination or turbidimetric immunoassays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat cross-linker should I use for protein coupling?\u003c\/strong\u003e\u003cbr\u003eEDC\/NHS is most common and gentle on proteins. Glutaraldehyde is more aggressive but can provide higher cross-linking density. Consult published protocols or commercial coupling kits for your specific protein.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVignali DA. Multiplexed particle-based flow cytometric assays. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2000;243(1-2):243-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(00)00238-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. \u003cem\u003eExp Hematol.\u003c\/em\u003e 2002;30(11):1227-37.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0301-472x(02)00922-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKrishhan VV, Khan IH, Luciw PA. Multiplexed microbead immunoassays by flow cytometry for molecular profiling: Basic concepts and proteomics applications. \u003cem\u003eCrit Rev Biotechnol.\u003c\/em\u003e 2009;29(1):29-43.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1080\/07388550802688847\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZhang B, Xiao G, Mao YQ, Lv Z, Huang RP. Cytometry Multiplex Bead Antibody Array. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2021;2237:83-92.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-1064-0_7\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805758976298,"sku":"BTS-B202642","price":1055.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B202642.png?v=1790801833","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-4-0-4-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}