{"product_id":"amine-modified-polystyrene-latex-beads-3-0-3-9-um","title":"Amine-Modified Polystyrene Latex Beads (3.0–3.9 μm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-Modified Polystyrene Latex Beads (3.0–3.9 μm) – Catalog #B202648\u003c\/h2\u003e\n\u003cp\u003eAmine-Modified Polystyrene Latex Beads (Catalog #B202648) are carboxyl-free, monodisperse microspheres with amino groups covalently attached to the surface. The 3.0–3.9 μm size range offers a balance between high surface area per unit volume and adequate visibility and handling in most optical and separation systems. These beads are ideal for applications where protein binding capacity and assay sensitivity are both critical.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB202648\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e3.0–3.9 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface modification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine groups (NH₂)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension in phosphate-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMultiplexed flow cytometric immunoassays, protein immobilization, solid-phase binding assays, turbidimetric assays, cell-surface marker detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-modified polystyrene beads, amine latex microspheres, amino polystyrene particles, amine-functionalized latex beads, amine-coated microspheres, polystyrene latex, protein-binding beads, flow cytometry beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePolystyrene latex beads have become a standard tool in biological research because their uniform size, smooth surface, and optical properties make them ideal carriers for binding proteins, nucleic acids, and small molecules. The addition of amine groups to the surface converts these inert particles into reactive carriers suitable for covalent immobilization of biomolecules.\u003c\/p\u003e\n\u003cp\u003eAmine groups react readily with carboxyl groups (via EDC\/NHS cross-linking) and with aldehyde-modified molecules (Schiff base formation), allowing researchers to conjugate antibodies, antigens, and other proteins to the bead surface. The 3.0–3.9 μm size is large enough to be easily handled and visualized by light microscopy, while still offering significant surface area for biomolecule loading.\u003c\/p\u003e\n\u003cp\u003eMultiplexed bead-based assays, where different-sized beads are each coated with different antibodies or capture molecules, represent a key innovation in high-throughput protein detection. Because the beads are distinguishable by size and\/or fluorescent internal labeling, a single flow cytometer run can measure dozens of targets simultaneously from a single sample.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMultiplexed flow cytometric immunoassays (sandwich assays for cytokines, growth factors, and other proteins)\u003c\/li\u003e\n\u003cli\u003eSolid-phase capture of antibodies or antigens for turbidimetric immunoassays\u003c\/li\u003e\n\u003cli\u003eAffinity purification of target proteins from crude cell lysates or tissue extracts\u003c\/li\u003e\n\u003cli\u003eFunctional studies of ligand–receptor interactions\u003c\/li\u003e\n\u003cli\u003ePreparation of custom immunological reagents\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C in the dark. The beads will settle over time; invert the bottle gently to resuspend before use. Do not vortex vigorously, as this can cause aggregation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein coupling:\u003c\/strong\u003e Amine groups are most effectively coupled to proteins via NHS ester activation or EDC\/NHS chemistry. Optimized protocols and coupling kits are available from multiple commercial suppliers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody immobilization:\u003c\/strong\u003e Use anti-species antibodies (e.g., anti-mouse IgG, anti-rabbit IgG) to coat the beads for sandwich immunoassays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Use gentle centrifugation (e.g., 2,000 × g for 2 minutes) rather than harsh magnetic separation to preserve bead integrity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Ensure beads are adequately resuspended and instrument gain settings are optimized for the bead size; acquire 10,000–50,000 bead events per sample.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking and storage:\u003c\/strong\u003e After protein conjugation, store bead conjugates at 4°C in phosphate-buffered saline containing 0.05% sodium azide and 1% bovine serum albumin to prevent aggregation and microbial contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of amine-modified polystyrene latex beads, 3.0–3.9 μm diameter\u003c\/li\u003e\n\u003cli\u003eUniform monodisperse particles suitable for multiplexing\u003c\/li\u003e\n\u003cli\u003eReactive amine surface for protein immobilization\u003c\/li\u003e\n\u003cli\u003eReady-to-use suspension in phosphate-buffered saline\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eWell-defined, uniform size ideal for optical sorting and flow cytometry\u003c\/li\u003e\n\u003cli\u003eAmine surface for straightforward covalent protein conjugation\u003c\/li\u003e\n\u003cli\u003eCompatible with standard immunoassay protocols and multiplexing workflows\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity and minimal background in typical assay conditions\u003c\/li\u003e\n\u003cli\u003eDecades of published literature supporting bead-based multiplex assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I conjugate proteins to these beads?\u003c\/strong\u003e\u003cbr\u003eAmine groups on the bead surface are most commonly conjugated using NHS ester or EDC\/NHS cross-linking chemistry. Commercial kits for protein conjugation to amine beads are available from Luminex, Bio-Rad, and other suppliers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for magnetic separation?\u003c\/strong\u003e\u003cbr\u003eThese beads are polystyrene and not inherently magnetic. For magnetic separation workflows, look for magnetic amine-modified beads, or coat these beads with streptavidin and use biotin-labeled magnetic particles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the particle concentration?\u003c\/strong\u003e\u003cbr\u003eParticle concentration varies by lot. Request the certificate of analysis (COA) or technical data sheet (TDS) for lot-specific particle count.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent bead aggregation during storage?\u003c\/strong\u003e\u003cbr\u003eStore at 2–8°C, protected from light, and avoid repeated freeze–thaw cycles. After protein conjugation, addition of bovine serum albumin and sodium azide helps maintain stability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these for ELISA or other solid-phase immunoassays?\u003c\/strong\u003e\u003cbr\u003eYes. These beads work well in ELISA-like formats where capture antibodies are immobilized, but the bead format offers advantages in multiplexing and automation compared to traditional microtiter plates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do these compare to magnetic beads?\u003c\/strong\u003e\u003cbr\u003eAmine polystyrene beads offer simpler handling and no magnetic interference, but magnetic beads allow faster, field-based separation. Choice depends on your workflow and instrumentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVignali DA. Multiplexed particle-based flow cytometric assays. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 2000;243(1-2):243-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(00)00238-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. \u003cem\u003eExp Hematol.\u003c\/em\u003e 2002;30(11):1227-37.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0301-472x(02)00922-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKumada Y, Miyamura Y, Tanibata R, Takahashi K, Ogasawara S, Gondaira F, Horiuchi JI. Design and site-directed immobilization of single-chain Fv antibody to polystyrene latex beads via material-binding peptides and application to latex turbidimetric assay. \u003cem\u003eJ Biosci Bioeng.\u003c\/em\u003e 2021;131(1):84-89.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiosc.2020.08.014\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGraham H, Chandler DJ, Dunbar SA. The genesis and evolution of bead-based multiplexing. \u003cem\u003eMethods.\u003c\/em\u003e 2019;158:2-11.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ymeth.2019.01.007\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805756485930,"sku":"BTS-B202648","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B202648.png?v=1790801825","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-3-0-3-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}