{"product_id":"amine-modified-polystyrene-latex-beads-1-0-1-9-um","title":"Amine-modified Polystyrene Latex Beads (1.0–1.9 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-modified Polystyrene Latex Beads (1.0–1.9 µm) – Catalog #B2025493\u003c\/h2\u003e\n\u003cp\u003eAmine-modified Polystyrene Latex Beads (Catalog #B2025493) are monodisperse, spherical particles (1.0–1.9 µm diameter) with primary amine groups grafted to the polystyrene surface. Supplied as 1 mL solution, these larger microspheres provide a platform for high-capacity protein immobilization, whole-cell labeling, and immunoassays where large bead size is advantageous for optical detection and manipulation.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025493\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1.0–1.9 µm (1000–1900 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePrimary amine (–NH2) groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-capacity protein immobilization, antibody-coated beads for immunoassays, cell labeling and tracking, bead-based enzyme assays, optical detection platforms, immunomagnetic separation (when combined with magnetic fields)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-functionalized polystyrene latex beads, amine-treated polystyrene latex particles, amine-modified PS latex beads, amine-enhanced polystyrene microspheres, amine-terminated polystyrene latex spheres, amine-substituted polystyrene latex beads, amine-activated polystyrene latex particles, 1 micrometer polystyrene beads, 1.9 micrometer beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePolystyrene microspheres in the 1–2 µm size range represent the largest diameter suitable for suspension-phase immunoassays, while remaining in the domain where individual beads can be observed and manipulated under standard light microscopy. At this size, each bead provides an exceptionally large surface area for ligand immobilization: a 1.5 µm microsphere has approximately 10–50 times the surface area of a 100 nm nanoparticle of the same material. This is advantageous when high protein loading capacity is required or when optical visualization of individual bead-ligand conjugates is desirable.\u003c\/p\u003e\n\u003cp\u003eAmine surface chemistry is stable and reactive, enabling straightforward coupling to proteins, antibodies, nucleic acids, and small-molecule ligands via standard carbodiimide (EDC\/NHS), reductive amination, or succinimide ester chemistry. The large particle size also makes these beads amenable to microfluidic platforms, flow sorting, and automated liquid handling systems.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-capacity protein and antibody immobilization platforms\u003c\/li\u003e\n\u003cli\u003eBead-based enzyme assays with high substrate capacity\u003c\/li\u003e\n\u003cli\u003eCell labeling and tracking (with fluorescent or magnetic derivatives)\u003c\/li\u003e\n\u003cli\u003eImmunomagnetic cell separation (when incorporated with magnetic materials)\u003c\/li\u003e\n\u003cli\u003eOptical detection and manipulation platforms (microarray, microfluidics)\u003c\/li\u003e\n\u003cli\u003eAgglutination assays where large bead size enhances optical readout\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C in the supplied buffer. Vortex gently or invert to resuspend beads that may have settled. Do not sonicate, as aggressive sonication can break or aggregate these larger microspheres.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein coupling:\u003c\/strong\u003e Wash beads 2–3 times in PBS or TBS to remove storage buffer. Incubate with protein and EDC\/NHS at a molar ratio of 1:4 to 1:10 (EDC:protein), depending on the desired loading. Incubate for 2–4 hours at room temperature or overnight at 4°C with gentle mixing to prevent bead settling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-capacity loading:\u003c\/strong\u003e The large surface area allows protein loading from 100 to 1000+ µg per mL of bead suspension, depending on the protein size and coupling efficiency. Optimize by performing a titration with your target protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e After coupling, block free amine groups with glycine (1 M, pH 8) or ethanolamine (1 M, pH 8) for 20–30 minutes at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuspension stability:\u003c\/strong\u003e These beads settle relatively quickly in water or low-salt buffers. Resuspend by gentle vortexing before each use, and minimize settling time during assays by maintaining gentle agitation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of amine-modified polystyrene latex beads (1.0–1.9 µm), supplied as solution\u003c\/li\u003e\n\u003cli\u003eMonodisperse microspheres with uniform, reactive amine surface\u003c\/li\u003e\n\u003cli\u003eMaximum surface area for high-capacity protein immobilization\u003c\/li\u003e\n\u003cli\u003eVisible under standard light microscopy for individual bead tracking\u003c\/li\u003e\n\u003cli\u003eReady-to-use solution—no preparation or activation required\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMaximum surface area (1.0–1.9 µm size) for high protein loading capacity\u003c\/li\u003e\n\u003cli\u003eIndividual beads visible under light microscopy for direct optical detection\u003c\/li\u003e\n\u003cli\u003eAmine surface enables rapid, efficient covalent protein immobilization\u003c\/li\u003e\n\u003cli\u003eBiologically inert polystyrene minimizes nonspecific interactions\u003c\/li\u003e\n\u003cli\u003eCompatible with manual and automated assay platforms, microfluidics, and optical imaging\u003c\/li\u003e\n\u003cli\u003eDecades of literature demonstrating stable, high-capacity bioconjugates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the protein loading capacity?\u003c\/strong\u003e\u003cbr\u003eDepends on protein size and coupling efficiency. Typical loading ranges from 100 to 1000+ µg protein per mL of bead suspension. Request a COA\/TDS or perform a titration with your target protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I see these beads under a microscope?\u003c\/strong\u003e\u003cbr\u003eYes. At 1.0–1.9 µm, individual beads are easily visible under a standard light microscope at 40× magnification or higher.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long does protein coupling take?\u003c\/strong\u003e\u003cbr\u003eTypically 2–4 hours at room temperature or overnight at 4°C with EDC\/NHS. Optimize by testing different incubation times and bead concentrations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy do my beads settle?\u003c\/strong\u003e\u003cbr\u003eAt this larger size, beads settle faster in low-salt buffers. Resuspend by gentle vortexing before each use and maintain gentle agitation during long incubations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in a magnetic separation system?\u003c\/strong\u003e\u003cbr\u003eNot directly—these beads are not magnetic. However, if you couple them to magnetic particles or coat them with a magnetic layer, they can be used in magnetic separation workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eXianyu Y, Dong Y, Wang Z, Xu Z, Huang R, Chen Y. Broad-Range Magnetic Relaxation Switching Bioassays Using Click Chemistry-Mediated Assembly of Polystyrene Beads and Magnetic Nanoparticles. \u003cem\u003eACS Sens.\u003c\/em\u003e 2019;4(7):1942-1949.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/acssensors.9b00900\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eYao Q, Gao Y, Gao T, Zhang Y, Harnoode C, Dong A, Liu Y, Xiao L. Surface arming magnetic nanoparticles with amine N-halamines as recyclable antibacterial agents: Construction and evaluation. \u003cem\u003eColloids Surf B Biointerfaces.\u003c\/em\u003e 2016;144:319-326.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.colsurfb.2016.04.024\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSheng Y, Liao LD, Thakor NV, Tan MC. Nanoparticles for molecular imaging. \u003cem\u003eJ Biomed Nanotechnol.\u003c\/em\u003e 2014;10(10):2641-76.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1166\/jbn.2014.1937\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805760745770,"sku":"BTS-B2025493","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025493.png?v=1790801852","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-1-0-1-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}