{"product_id":"amine-modified-polystyrene-latex-beads-0-2-0-5-um-2-5-w-v","title":"Amine-Modified Polystyrene Latex Beads (0.2–0.5 µm, 2.5% w\/v)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-Modified Polystyrene Latex Beads (0.2–0.5 µm, 2.5% w\/v) – Catalog #B2025659\u003c\/h2\u003e\n\u003cp\u003eAmine-Modified Polystyrene Latex Beads (Catalog #B2025659) are uniformly sized, spherical particles (200–500 nm diameter) with amine functional groups covalently bound to the polystyrene surface. Supplied as a 2.5% w\/v suspension in 5 mL, these beads offer a ready-to-use platform for flow cytometry, immunoassays, protein immobilization, and diagnostic applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025659\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e0.2–0.5 µm (200–500 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2.5% w\/v\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePrimary amine (–NH2) groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eFlow cytometry, immunoassays, protein and antibody immobilization, biomarker detection, fluorescence labeling, diagnostic assay development, multiplex bead-based assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-modified polystyrene microspheres, amine-functionalized polystyrene latex, amine-enhanced polystyrene beads, amine-modified PS latex particles, amine-functional polystyrene beads, amine-modified polystyrene spheres, amine-modified PS microspheres, polystyrene microspheres, 200 nm beads, 500 nm beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePolystyrene latex microspheres in the 200–500 nm size range occupy an important niche between smaller nanoparticles and larger microbeads. This size provides moderate surface area, good particle dispersity, and efficient binding kinetics in solution-phase immunoassays. The 2.5% w\/v formulation is a standard concentration that balances particle availability with ease of handling and resuspension.\u003c\/p\u003e\n\u003cp\u003eAmine surface chemistry enables facile covalent coupling to proteins and antibodies using EDC\/NHS or other amine-reactive cross-linkers. In flow cytometry, these beads serve both as compensator beads (for instrument standardization) and as platforms for multiplex assays where different bead subsets are distinguished by size and internal fluorescence and each subset carries a different capture antibody.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eFlow cytometry compensation and standardization beads\u003c\/li\u003e\n\u003cli\u003eMultiplex bead-based immunoassays (e.g., Luminex platforms)\u003c\/li\u003e\n\u003cli\u003eELISA and sandwich immunoassay platforms\u003c\/li\u003e\n\u003cli\u003eFluorescence-based protein labeling and detection\u003c\/li\u003e\n\u003cli\u003eCell-free in vitro diagnostic assays\u003c\/li\u003e\n\u003cli\u003eBiomarker enrichment and detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. The 2.5% w\/v concentration provides a convenient working stock; vortex gently before each use to resuspend settled beads. Do not sonicate aggressively, as this can damage the surface or reduce the monodispersity.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein coupling:\u003c\/strong\u003e Wash beads 2–3 times in PBS to exchange the storage buffer. Incubate with protein and EDC\/NHS at a molar ratio optimized for your ligand. Typical coupling times are 2–4 hours at room temperature or overnight at 4°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDilution:\u003c\/strong\u003e For low-concentration assays, dilute beads in PBS or Tris-buffered saline (TBS) just before use. Extreme dilution may promote aggregation; prepare fresh working dilutions daily.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling efficiency:\u003c\/strong\u003e Request a COA\/TDS for lot-specific binding data, or perform a test coupling with your target protein to optimize conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e After coupling, block free amine groups with glycine (1 M, pH 8) for 20–30 minutes to reduce nonspecific binding.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of amine-modified polystyrene latex beads (200–500 nm), 2.5% w\/v suspension\u003c\/li\u003e\n\u003cli\u003eMonodisperse, uniformly sized particles with reactive amine surface\u003c\/li\u003e\n\u003cli\u003eConvenient, ready-to-use concentration for standard assay applications\u003c\/li\u003e\n\u003cli\u003eOptimal size for flow cytometry and moderate-throughput immunoassays\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eModerate size (200–500 nm) balances surface area with assay compatibility\u003c\/li\u003e\n\u003cli\u003e2.5% w\/v concentration is ideal for flow cytometry and multiplex assays\u003c\/li\u003e\n\u003cli\u003eAmine surface enables rapid, straightforward protein immobilization\u003c\/li\u003e\n\u003cli\u003eBiologically inert polystyrene minimizes nonspecific protein binding\u003c\/li\u003e\n\u003cli\u003eWide compatibility with commercial flow cytometers and multiplex platforms (Luminex, etc.)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the exact particle concentration in this 2.5% w\/v suspension?\u003c\/strong\u003e\u003cbr\u003eConcentration varies by particle size and density. Request a COA\/TDS for the particles-per-mL specification for your lot.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in a Luminex multiplex assay?\u003c\/strong\u003e\u003cbr\u003eYes, if the bead size and concentration are within the instrument's specifications. Consult the instrument manual and request a COA confirming Luminex compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prepare beads for coupling?\u003c\/strong\u003e\u003cbr\u003eWash 2–3 times in PBS by centrifuging and resuspending. Discard the supernatant and proceed with EDC\/NHS coupling in the desired buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the maximum protein loading?\u003c\/strong\u003e\u003cbr\u003eDepends on the protein size and the cross-linker stoichiometry. Perform a titration with your target protein to find the saturation point, or request lot-specific binding data from us.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I couple nucleic acids to these beads?\u003c\/strong\u003e\u003cbr\u003eYes. Use amine-to-carboxyl coupling if your DNA or RNA has 5' or 3' phosphate or carboxyl groups, or use amine-reactive linkers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eXianyu Y, Dong Y, Wang Z, Xu Z, Huang R, Chen Y. Broad-Range Magnetic Relaxation Switching Bioassays Using Click Chemistry-Mediated Assembly of Polystyrene Beads and Magnetic Nanoparticles. \u003cem\u003eACS Sens.\u003c\/em\u003e 2019;4(7):1942-1949.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/acssensors.9b00900\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eChen H, Yang F, Yin G, Song P. Nanomaterial-assisted determination of osteosarcoma by antibody-osteopontin-aptamer sandwich ELISA. \u003cem\u003eBiotechnol Appl Biochem.\u003c\/em\u003e 2022;69(4):1646-1652.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/bab.2234\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKim S, Pyo HB, Ko SH, Ah CS, Kim A, Kim WJ. Fabrication of anionic sulfate-functionalized nanoparticles as an immunosensor by protein immobilization. \u003cem\u003eLangmuir.\u003c\/em\u003e 2010;26(10):7355-64.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/la9043717\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759926570,"sku":"BTS-B2025659","price":705.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025659.png?v=1790801843","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-0-2-0-5-um-2-5-w-v","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}