{"product_id":"amine-modified-polystyrene-latex-beads-0-05-0-1-um","title":"Amine-modified Polystyrene Latex Beads (0.05–0.1 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine-modified Polystyrene Latex Beads (0.05–0.1 µm) – Catalog #B2025485\u003c\/h2\u003e\n\u003cp\u003eAmine-modified Polystyrene Latex Beads (Catalog #B2025485) are monodisperse nanoparticles (50–100 nm diameter) composed of polystyrene with primary amine functional groups on the surface. Supplied as 5 mL solution, these beads are widely used in diagnostic assay development, immunology, and cell biology as platforms for protein immobilization, antigen or antibody labeling, and high-sensitivity detection.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025485\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e0.05–0.1 µm (50–100 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePrimary amine (–NH2) groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eELISA and immunoassay platforms, flow cytometry calibration and compensation, protein labeling, antibody capture beads, diagnostic assay development, agglutination assays, vaccine development\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-functionalized polystyrene latex beads, amine-treated polystyrene latex particles, amine-modified PS latex beads, amine-enhanced polystyrene latex spheres, amine-terminated polystyrene latex beads, amine-substituted polystyrene latex particles, amine-activated polystyrene latex beads, polystyrene nanoparticles, amino polystyrene beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePolystyrene latex beads are well-characterized, biologically inert particles that serve as a universal platform for protein immobilization and diagnostics. Their nanometer-scale size (50–100 nm) provides a very high surface-area-to-volume ratio, enabling efficient and rapid binding of protein ligands. Because polystyrene does not interact nonspecifically with most proteins, beads derivatized with specific antibodies or antigens can be used directly in diagnostic assays with minimal background.\u003c\/p\u003e\n\u003cp\u003eAmine modification introduces primary amino groups on the bead surface, which are nucleophilic and react readily with activated carboxyls (via EDC\/NHS), aldehydes (via reductive amination), succinimide esters, and isothiocyanates. This chemical versatility has made amine-modified polystyrene the reference standard for immunodiagnostic bead development, flow cytometry, and high-throughput screening platforms.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSandwich ELISA and capture-based immunoassays\u003c\/li\u003e\n\u003cli\u003eFlow cytometry controls and compensation beads\u003c\/li\u003e\n\u003cli\u003eImmunomagnetic separation (when coupled with magnetic beads)\u003c\/li\u003e\n\u003cli\u003eProtein labeling and fluorescence immunoassays\u003c\/li\u003e\n\u003cli\u003eVaccine development and immune response monitoring\u003c\/li\u003e\n\u003cli\u003eAgglutination assays and lateral-flow diagnostic devices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Vortex gently before use to resuspend beads that may have settled. Avoid prolonged sonication, which can damage the surface or break particle agglomerates.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein immobilization:\u003c\/strong\u003e Wash beads 2–3 times in PBS (phosphate-buffered saline) to remove storage buffer, then incubate with the ligand (protein, antibody, or antigen) and appropriate cross-linker (e.g., EDC\/NHS at molar ratios of 1:4 to 1:10). Allow 1–4 hours coupling time at room temperature or 4°C overnight.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e After coupling, block remaining amine groups with glycine or ethanolamine to reduce nonspecific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e Request a COA\/TDS for the suspension concentration (particles per mL), which is critical for downstream applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimization:\u003c\/strong\u003e Protein loading depends on the molar ratio of cross-linker, ligand, and bead concentration. Perform a titration to optimize binding and minimize aggregation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of amine-modified polystyrene latex beads (50–100 nm), supplied as solution\u003c\/li\u003e\n\u003cli\u003eMonodisperse nanoparticles with uniform, reactive amine surface\u003c\/li\u003e\n\u003cli\u003eHigh surface area for efficient protein immobilization and labeling\u003c\/li\u003e\n\u003cli\u003eReady-to-use suspension—no preparation or activation required\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eWell-characterized, biologically inert polystyrene platform with decades of diagnostic use\u003c\/li\u003e\n\u003cli\u003eSmall size (50–100 nm) maximizes surface area and minimizes aggregation in assays\u003c\/li\u003e\n\u003cli\u003eAmine surface enables rapid, straightforward covalent coupling to proteins and antibodies\u003c\/li\u003e\n\u003cli\u003eConsistent monodispersity ensures reproducible assay performance\u003c\/li\u003e\n\u003cli\u003eCompatible with standard immunoassay workflows, flow cytometry, and diagnostic devices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the particle size?\u003c\/strong\u003e\u003cbr\u003e50–100 nm diameter, chosen for high surface area and fast reaction kinetics in diagnostic assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow many particles are in the suspension?\u003c\/strong\u003e\u003cbr\u003eRequest a COA\/TDS for the concentration in particles per mL, which is essential for calculating ligand-to-bead molar ratios.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I wash the beads?\u003c\/strong\u003e\u003cbr\u003eYes. Centrifuge at 10,000–20,000 × g for 10–15 minutes, discard supernatant, and resuspend in fresh buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long does protein coupling take?\u003c\/strong\u003e\u003cbr\u003eTypically 1–4 hours at room temperature or overnight at 4°C with EDC\/NHS. Optimize by testing different incubation times and bead concentrations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent nonspecific binding?\u003c\/strong\u003e\u003cbr\u003eBlock unreacted amine groups after coupling with glycine (1 M, pH 7–8) for 30 minutes, then wash thoroughly.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eXianyu Y, Dong Y, Wang Z, Xu Z, Huang R, Chen Y. Broad-Range Magnetic Relaxation Switching Bioassays Using Click Chemistry-Mediated Assembly of Polystyrene Beads and Magnetic Nanoparticles. \u003cem\u003eACS Sens.\u003c\/em\u003e 2019;4(7):1942-1949.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/acssensors.9b00900\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKim S, Pyo HB, Ko SH, Ah CS, Kim A, Kim WJ. Fabrication of anionic sulfate-functionalized nanoparticles as an immunosensor by protein immobilization. \u003cem\u003eLangmuir.\u003c\/em\u003e 2010;26(10):7355-64.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/la9043717\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKetmen S, Er Zeybekler S, Gelen SS, Odaci D. Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein. \u003cem\u003eBiosensors (Basel).\u003c\/em\u003e 2022;12(12).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3390\/bios12121175\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759172906,"sku":"BTS-B2025485","price":1215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025485.png?v=1790801836","url":"https:\/\/bluetigerscientific.com\/products\/amine-modified-polystyrene-latex-beads-0-05-0-1-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}