{"product_id":"amine-magnetic-beads-1-um","title":"Amine Magnetic Beads (1 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine Magnetic Beads (1 µm) – Catalog #B2025653\u003c\/h2\u003e\n\u003cp\u003eAmine Magnetic Beads (Catalog #B2025653) are monodisperse polymer or composite beads (1 µm diameter) with superparamagnetic iron oxide embedded in their core and primary amine groups on the surface. Supplied as 1 mL of suspension, these beads rapidly respond to external magnetic fields and provide a high-surface-area platform for biomolecule immobilization and magnetic separation.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025653\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePrimary amine (–NH2) groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic sample preparation, immunoassays, protein immobilization, antibody capture, DNA\/RNA extraction, cell isolation, biomarker enrichment, diagnostic assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-coated magnetic beads, amine-functionalized magnetic particles, amine magnetic nanoparticles, amine-modified magnetic beads, amino magnetic beads, amino-functional magnetic particles, amino-coated magnetic beads, 1 micrometer magnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eMagnetic beads have become the workhorse platform for high-throughput sample preparation and separation in clinical diagnostics, molecular biology, and proteomics. Their 1 µm size offers a practical balance: large enough to be separated efficiently in seconds with handheld or automated magnetic racks (1–2 Tesla fields), yet small enough to provide high surface area per unit volume for ligand immobilization and rapid reaction kinetics. The embedded superparamagnetic core (iron oxide) ensures that the beads are attracted to magnetic fields but do not aggregate in the absence of a field.\u003c\/p\u003e\n\u003cp\u003eAmine surface chemistry enables straightforward covalent attachment of proteins, antibodies, enzymes, aptamers, and nucleic acids using standard cross-linking reagents (EDC\/NHS for carboxyl coupling) or other primary amine-reactive chemistries. This makes amine-functionalized beads the platform of choice for building biomolecule-capture systems with defined binding kinetics and specificity.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid immunomagnetic capture of antibodies, antigens, and antibody-antigen complexes\u003c\/li\u003e\n\u003cli\u003eMagnetic sample preparation for proteomics and genomics workflows\u003c\/li\u003e\n\u003cli\u003eCell isolation and enrichment (via magnetic labeling of cell-surface antigens)\u003c\/li\u003e\n\u003cli\u003eEnzyme immobilization with magnetic recovery for biocatalysis\u003c\/li\u003e\n\u003cli\u003eHigh-throughput biomarker screening and diagnostic assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C. Vortex gently or invert several times before use to resuspend the beads. Particles settle over time; resuspend before each aliquot.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a magnetic separator rated for 1 µm particles (e.g., Dynal, Invitrogen, or equivalent). Typical separation time is 1–2 minutes with a 1 Tesla field.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBioconjugation:\u003c\/strong\u003e The amine surface is compatible with EDC\/NHS cross-linking, amine-reactive NHS esters, and other standard chemistries. Titrate the bead concentration and cross-linker to avoid over-functionalization, which can reduce binding specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein loading:\u003c\/strong\u003e Request a COA\/TDS for the maximum protein binding capacity per unit mass of beads.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e After magnetic separation, wash beads in suspension by resuspending in buffer and re-separating 2–3 times to remove unbound material.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of amine-coated magnetic beads (1 µm), supplied as suspension\u003c\/li\u003e\n\u003cli\u003eSuperparamagnetic beads for rapid magnetic separation\u003c\/li\u003e\n\u003cli\u003eHigh surface area for efficient biomolecule immobilization\u003c\/li\u003e\n\u003cli\u003eReady-to-use suspension—no synthesis or coating required\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 µm size balances high surface area with rapid, complete magnetic separation\u003c\/li\u003e\n\u003cli\u003eAmine surface enables straightforward covalent immobilization of proteins, antibodies, and nucleic acids\u003c\/li\u003e\n\u003cli\u003eConsistent, monodisperse particles ensure reproducible immobilization and reaction kinetics\u003c\/li\u003e\n\u003cli\u003eRapid sample preparation in automated or manual magnetic separation workflows\u003c\/li\u003e\n\u003cli\u003eWell-established protocols across diagnostic, genomic, and proteomic applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long does magnetic separation take?\u003c\/strong\u003e\u003cbr\u003eTypically 1–2 minutes with a standard magnetic separator and 1 mL sample volume. Smaller volumes separate faster.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the binding capacity?\u003c\/strong\u003e\u003cbr\u003eDepends on the ligand and surface coverage. Request a COA\/TDS for lot-specific binding data, or perform a titration with your target protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in an automated separator?\u003c\/strong\u003e\u003cbr\u003eYes. 1 µm beads work with commercial magnetic racks (Dynal, KingFisher, Invitrogen) designed for beads in this size range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent bead clumping?\u003c\/strong\u003e\u003cbr\u003eGently vortex or invert before each use. Avoid prolonged standing without resuspension. If clumping occurs, sonicate gently or vortex again.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat cross-linker should I use?\u003c\/strong\u003e\u003cbr\u003eEDC\/NHS is a standard choice for amine-to-carboxyl coupling. For other cross-linking chemistries, consult protocols or request recommendations from us.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHola K, Markova Z, Zoppellaro G, Tucek J, Zboril R. Tailored functionalization of iron oxide nanoparticles for MRI, drug delivery, magnetic separation and immobilization of biosubstances. \u003cem\u003eBiotechnol Adv.\u003c\/em\u003e 2015;33(6 Pt 2):1162-76.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.biotechadv.2015.02.003\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSingh S, Barick KC, Bahadur D. Surface engineered magnetic nanoparticles for removal of toxic metal ions and bacterial pathogens. \u003cem\u003eJ Hazard Mater.\u003c\/em\u003e 2011;192(3):1539-47.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jhazmat.2011.06.074\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCh'ng ACW, Hamidon NHB, Konthur Z, Lim TS. Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2018;1701:301-319.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-7447-4_16\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKim KS, Park JK. Magnetic force-based multiplexed immunoassay using superparamagnetic nanoparticles in microfluidic channel. \u003cem\u003eLab Chip.\u003c\/em\u003e 2005;5(6):657-64.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/b502225h\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805756748074,"sku":"BTS-B2025653","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025653.png?v=1790801827","url":"https:\/\/bluetigerscientific.com\/products\/amine-magnetic-beads-1-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}