{"product_id":"amine-iron-oxide-nanoparticles-5-30-nm","title":"Amine Iron Oxide Nanoparticles (5–30 nm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAmine Iron Oxide Nanoparticles (5–30 nm) – Catalog #B2026063\u003c\/h2\u003e\n\u003cp\u003eAmine Iron Oxide Nanoparticles (Catalog #B2026063) are colloidal suspensions of superparamagnetic iron oxide nanoparticles (5–30 nm diameter) with primary amine groups grafted onto their surfaces. These particles are supplied as 1 mL of liquid suspension and are widely used in biomedical and research applications that exploit both the magnetic and surface-reactive properties of functionalized iron oxide.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026063\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5–30 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLiquid suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePrimary amine (–NH2) groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic separation, immunoassays, protein immobilization, enzyme immobilization, cell tracking, bioconjugation, drug delivery, diagnostics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmine-coated iron oxide nanoparticles, amino iron oxide nanoparticles, amine-functionalized iron oxide nanoparticles, iron oxide nanoparticles with amine groups, amine-modified iron oxide nanoparticles, iron oxide nanoparticles with amino functionality, superparamagnetic iron oxide nanoparticles, SPION\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eIron oxide nanoparticles are composed of Fe₃O₄ or γ-Fe₂O₃ and exhibit superparamagnetic behavior: they acquire a magnetic moment when exposed to an external field but return to zero magnetization when the field is removed. This prevents aggregation in the absence of a field while allowing rapid magnetic separation when needed. The amine surface coating is introduced via silane chemistry or polymer coating to enable covalent bioconjugation. Amine groups are nucleophilic and can be coupled to carboxyl groups using carbodiimides (EDC\/NHS), to aldehyde groups via reductive amination, or to maleimides and other activated esters.\u003c\/p\u003e\n\u003cp\u003eIron oxide nanoparticles have been extensively studied for applications spanning magnetic resonance imaging, magnetic cell separation, enzyme immobilization, and magnetic immunoassays. Surface functionalization—particularly with amine groups—is critical to directing the particles' behavior in biological systems and enabling bioconjugation strategies tailored to specific applications.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eMagnetic protein and antibody immobilization for immunoassays and affinity purification\u003c\/li\u003e\n\u003cli\u003eEnzyme immobilization and biocatalysis with magnetic recovery\u003c\/li\u003e\n\u003cli\u003eRapid magnetic cell and bacterial separation in diagnostic assays\u003c\/li\u003e\n\u003cli\u003eBioconjugation via covalent attachment of targeting molecules, aptamers, or peptides\u003c\/li\u003e\n\u003cli\u003eMagnetic drug delivery and cell labeling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C. Sonicate or vortex gently before use to ensure uniform dispersion. Avoid prolonged storage without agitation to prevent settling. Protect from strong magnetic fields during storage.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBioconjugation:\u003c\/strong\u003e The amine groups are stable and reactive. Use standard amine-to-carboxyl or amine-to-aldehyde cross-linking chemistry with commercial kits (EDC\/NHS for carboxyl coupling, reductive amination for aldehyde coupling).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Apply 0.5–1 minute of magnetic field separation; longer times can cause irreversible particle settling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration and aggregation:\u003c\/strong\u003e Particles are stable in aqueous suspension at working concentrations. Avoid extended dilution beyond manufacturer recommendations, which may promote aggregation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein binding capacity:\u003c\/strong\u003e Request a COA\/TDS for the lot-specific binding capacity and saturation conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of amine-coated iron oxide nanoparticles (5–30 nm), supplied as liquid suspension\u003c\/li\u003e\n\u003cli\u003eSuperparamagnetic particles with rapid magnetic response\u003c\/li\u003e\n\u003cli\u003eReactive amine surface for covalent bioconjugation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAmine surface enables straightforward covalent attachment of biomolecules without additional chemistry\u003c\/li\u003e\n\u003cli\u003eSuperparamagnetic behavior allows rapid, field-driven separation without aggregation\u003c\/li\u003e\n\u003cli\u003eNanoparticle size (5–30 nm) enables deep tissue penetration in cell-based and diagnostic assays\u003c\/li\u003e\n\u003cli\u003eDecades of literature demonstrating stable bioconjugates and reliable magnetic recovery\u003c\/li\u003e\n\u003cli\u003eReady-to-use liquid suspension—no lengthy synthesis or coating steps required\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat are the particles made of?\u003c\/strong\u003e\u003cbr\u003eIron oxide (Fe₃O₄ or γ-Fe₂O₃) with an amine-coated surface layer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I attach proteins or antibodies to these particles?\u003c\/strong\u003e\u003cbr\u003eUse amine-to-carboxyl cross-linking with EDC\/NHS, or use amine-specific reagents. Request a COA\/TDS for recommended starting concentrations and protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre the particles magnetic enough for separation?\u003c\/strong\u003e\u003cbr\u003eYes. They respond rapidly to 0.5–1 Tesla fields commonly used in clinical and research magnetic separation devices.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I store them long-term?\u003c\/strong\u003e\u003cbr\u003eStore at 2–8°C in the original container. Sonicate before each use to resuspend. Shelf life depends on the lot; consult the label or request a COA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat if the particles start to aggregate?\u003c\/strong\u003e\u003cbr\u003eGentle sonication usually restores suspension. If aggregation persists, the suspension may have exceeded its shelf life; contact us for a replacement lot.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLi Y, Wu W, Liu Q, Wu Q, Ren P, Xi X, Liu H, Zhao J, Zhang W, Wang Z, Lv Y, Tian B, Sun S, Cui J, Zhao Y, Wu J, Gao M, Chen F. Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity. \u003cem\u003eNat Commun.\u003c\/em\u003e 2024;15(1):10400.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/s41467-024-54810-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eChang YK, Liu YP, Ho JH, Hsu SC, Lee OK. Amine-surface-modified superparamagnetic iron oxide nanoparticles interfere with differentiation of human mesenchymal stem cells. \u003cem\u003eJ Orthop Res.\u003c\/em\u003e 2012;30(9):1499-506.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/jor.22088\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHola K, Markova Z, Zoppellaro G, Tucek J, Zboril R. Tailored functionalization of iron oxide nanoparticles for MRI, drug delivery, magnetic separation and immobilization of biosubstances. \u003cem\u003eBiotechnol Adv.\u003c\/em\u003e 2015;33(6 Pt 2):1162-76.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.biotechadv.2015.02.003\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eYuan Y, Rende D, Altan CL, Bucak S, Ozisik R, Borca-Tasciuc DA. Effect of surface modification on magnetization of iron oxide nanoparticle colloids. \u003cem\u003eLangmuir.\u003c\/em\u003e 2012;28(36):13051-9.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/la3022479\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805756059946,"sku":"BTS-B2026063","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026063-1.png?v=1790801819","url":"https:\/\/bluetigerscientific.com\/products\/amine-iron-oxide-nanoparticles-5-30-nm","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}