{"product_id":"alkali-tolerant-protein-a-magnetic-agarose-beads","title":"Alkali-Tolerant Protein A Magnetic Agarose Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eAlkali-Tolerant Protein A Magnetic Agarose Beads – Catalog #B2025483\u003c\/h2\u003e\n\u003cp\u003eAlkali-Tolerant Protein A Magnetic Agarose Beads (Catalog #B2025483) combine magnetic agarose particles with covalently immobilized Protein A, a ~42 kDa protein from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e strain Cowan I that binds the Fc domain of IgG with high affinity and specificity. The key innovation is alkali tolerance: the coupling chemistry and linker are engineered to resist degradation under alkaline pH (typically pH 12–13), allowing beads to be regenerated using mild alkaline buffers instead of harsh denaturants. This enables cost-effective reuse over many cycles without loss of Protein A activity. Supplied as 10 mL of suspension, these beads simplify large-scale and repetitive IgG purification.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025483\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLigand:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eProtein A (Staphylococcus aureus Cowan I)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBinding specificity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eIgG Fc region (all mammalian IgG subtypes)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAlkali-tolerance:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eStable at pH 12–13 for regeneration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAntibody (IgG) purification, affinity capture of IgG-tagged proteins, immunoprecipitation, rapid isolation of immunoglobulin from serum or culture supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eProtein A beads, Protein A magnetic beads, IgG purification, antibody purification, magnetic agarose, alkali-resistant, alkali-stable, immunoglobulin G affinity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eProtein A is a 42 kDa cell wall protein from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e strain Cowan I that contains five domains (E, D, A, B, C), each capable of binding the Fc region of IgG. This binding is species-independent but isoform-dependent: human IgG1, IgG2, and IgG4 bind tightly, while IgG3 and some non-mammalian IgGs may bind more weakly. Protein A has been used for decades in affinity chromatography and immunoprecipitation because of its high specificity, high binding capacity (~100 mg IgG per mL settled beads), and reversibility—IgG can be eluted under mild acidic conditions (pH 2–3) or by competitive displacement. The key innovation in these alkali-tolerant beads is the linker chemistry connecting Protein A to agarose. Standard Protein A beads require harsh denaturants (e.g., 6 M urea, 0.1 M acetic acid) for regeneration, which eventually degrade the ligand. Alkali-tolerant variants withstand repeated exposure to pH 12–13 buffers (e.g., 0.1 M glycine-NaOH), preserving Protein A activity over 20–50+ cycles.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid purification of IgG from serum, plasma, or cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eAffinity isolation of IgG-tagged recombinant proteins\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation (IP) of antibody-bound antigen complexes\u003c\/li\u003e\n\u003cli\u003eCost-effective, reusable affinity matrix for repeated large-scale antibody purifications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Before use, gently resuspend the beads by inverting the tube several times. Avoid vortexing. Use a standard magnetic separation rack for T1 or similar-sized beads.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eIgG binding:\u003c\/strong\u003e Incubate the bead suspension with your IgG-containing sample (serum, culture supernatant, or purified IgG) in neutral pH buffer (PBS or Tris) at room temperature or 4°C for 10–30 minutes with gentle mixing. Protein A binding is rapid and avid, reaching equilibrium quickly.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Place the tube in a magnetic rack and allow 1–2 minutes for beads to settle. Carefully aspirate the supernatant while beads are held magnetically.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Resuspend beads in neutral buffer, re-magnetize, and aspirate to remove non-specifically bound material. Repeat 2–3 times.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Resuspend beads in acidic buffer (e.g., 0.1 M glycine–HCl, pH 2.7, or 0.2 M citric acid, pH 3.0) for 2–5 minutes to elute IgG. Immediately neutralize the eluate with a buffering reagent (e.g., 1 M Tris–HCl, pH 8.0) to prevent IgG denaturation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e To regenerate beads for reuse, wash extensively with neutral buffer, then expose to alkaline buffer (0.1 M glycine–NaOH, pH 12–13) for 10 minutes, followed by extensive neutral buffer washing. The alkali-tolerant linker preserves Protein A activity; activity typically remains \u0026gt;80% after 20+ regeneration cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e10 mL of alkali-tolerant Protein A magnetic agarose beads, supplied as suspension\u003c\/li\u003e\n\u003cli\u003eHigh-capacity IgG affinity matrix (\u0026gt;100 mg IgG per mL settled beads)\u003c\/li\u003e\n\u003cli\u003eReusable over 20+ binding\/elution\/regeneration cycles\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAlkali-stable Protein A preserves activity over many reuse cycles, reducing cost per purification\u003c\/li\u003e\n\u003cli\u003eRapid magnetic separation eliminates centrifugation\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity and specificity for IgG\u003c\/li\u003e\n\u003cli\u003eCompatible with acidic and alkaline pH buffers for flexible regeneration strategies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow many times can I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eTypically 20–50+ cycles of binding, washing, elution, and regeneration with alkaline buffer. Activity gradually declines; monitor performance or contact our technical team for guidance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat IgG species does Protein A bind?\u003c\/strong\u003e\u003cbr\u003eProtein A binds all mammalian IgG subtypes with varying affinity (human IgG1, IgG2, IgG4 very tightly; IgG3 and non-mammalian IgGs variably). For bird, fish, or non-mammalian antibodies, test binding empirically or consider Protein G.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I elute with high salt instead of acid?\u003c\/strong\u003e\u003cbr\u003eYes, high-salt buffers (e.g., 1 M NaCl) elute IgG less completely than acidic pH. For quantitative recovery, use pH 2.7–3.0 elution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between alkali-tolerant and standard Protein A beads?\u003c\/strong\u003e\u003cbr\u003eStandard beads degrade under pH 12–13, requiring denaturant-based regeneration (urea, acid). Alkali-tolerant beads resist alkaline pH, preserving ligand and allowing mild regeneration and cost-effective reuse.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for other proteins tagged with IgG or Fc?\u003c\/strong\u003e\u003cbr\u003eYes, Protein A binds any Fc-tagged protein. Binding affinity depends on the Fc domain; human and mouse Fc are standard.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSalimi K, Usta DD, Koçer İ, Çelik E, Tuncel A. Protein A and protein A\/G coupled magnetic SiO(2) microspheres for affinity purification of immunoglobulin G. \u003cem\u003eInt J Biol Macromol.\u003c\/em\u003e 2018;111:178-185.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2018.01.019\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchotte L, Rombaut B, Thys B. A liquid phase affinity capture assay using magnetic beads to study protein-protein interaction: the poliovirus-nanobody example. \u003cem\u003eJ Vis Exp.\u003c\/em\u003e 2012;(63).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/3937\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eStoltenburg R, Schubert T, Strehlitz B. In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein A. \u003cem\u003ePLoS One.\u003c\/em\u003e 2015;10(7):e0134403.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1371\/journal.pone.0134403\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805762777386,"sku":"BTS-B2025483","price":1215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025483.png?v=1790801867","url":"https:\/\/bluetigerscientific.com\/products\/alkali-tolerant-protein-a-magnetic-agarose-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}