{"product_id":"aco2-antibody-center-rabbit-polyclonal","title":"ACO2 Antibody (Center), Rabbit Polyclonal","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eACO2 Antibody (Center), Rabbit Polyclonal – Catalog #L2001509\u003c\/h2\u003e\n\u003cp\u003eACO2 Antibody (Center) (Catalog #L2001509) is a purified rabbit polyclonal antibody against aconitate hydratase, mitochondrial (ACO2). It was generated by immunizing rabbits with a KLH-conjugated synthetic peptide corresponding to amino acids 438–467 in the central region of human ACO2, and is supplied as 100 µg in PBS with 0.09% (w\/v) sodium azide. The antibody reacts with human and rat ACO2 and is suitable for Western blotting (WB) and immunohistochemistry on paraffin-embedded sections (IHC-P).\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eL2001509\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e100 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e0.25–0.5 mg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eHost \/ Clonality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRabbit \/ Polyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eKLH-conjugated synthetic peptide, aa 438–467 (central region) of human ACO2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eWestern blot (WB), immunohistochemistry – paraffin (IHC-P)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003ePurification:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSaturated ammonium sulfate (SAS) precipitation followed by dialysis against PBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution in PBS with 0.09% (w\/v) sodium azide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTarget:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eACO2 (aconitate hydratase, mitochondrial; UniProt Q99798; EC 4.2.1.3)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eACO2 antibody, anti-ACO2, mitochondrial aconitase antibody, aconitate hydratase mitochondrial, aconitase 2, citrate hydro-lyase, EC 4.2.1.3, rabbit polyclonal ACO2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eACO2 encodes mitochondrial aconitase (aconitate hydratase), the second enzyme of the tricarboxylic acid (TCA) cycle, which catalyzes the stereospecific isomerization of citrate to isocitrate. The enzyme contains an iron–sulfur cluster that is sensitive to oxidative damage, so ACO2 abundance and activity are frequently examined in studies of mitochondrial function and oxidative stress.\u003c\/p\u003e\n\u003cp\u003eACO2 is located on chromosome 22q13.2. Biallelic pathogenic variants cause infantile cerebellar-retinal degeneration (ICRD) and have also been linked to optic atrophy, making ACO2 a target of interest in mitochondrial disease and neurodegeneration research.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDetecting ACO2 protein levels by Western blot in human and rat samples\u003c\/li\u003e\n\u003cli\u003eLocalizing ACO2 in paraffin-embedded tissue by IHC\u003c\/li\u003e\n\u003cli\u003eMitochondrial metabolism and TCA cycle research\u003c\/li\u003e\n\u003cli\u003eOxidative stress and mitochondrial disease studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at −20°C. Aliquot on first thaw to avoid repeated freeze–thaw cycles, and centrifuge briefly before opening. Optimal working dilutions should be determined by the end user for each application; request the datasheet for starting recommendations.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eSodium azide:\u003c\/strong\u003e Contains 0.09% sodium azide, which inhibits horseradish peroxidase. Keep azide out of HRP detection steps and handle with appropriate care.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include a positive-control lysate or tissue with known ACO2 expression when establishing conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e100 µg of purified rabbit polyclonal anti-ACO2 antibody\u003c\/li\u003e\n\u003cli\u003eSupplied at 0.25–0.5 mg\/mL in PBS with 0.09% sodium azide\u003c\/li\u003e\n\u003cli\u003eReactivity with human and rat ACO2\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDefined immunogen from the central region (aa 438–467) of human ACO2\u003c\/li\u003e\n\u003cli\u003eSuitable for both Western blot and IHC-P\u003c\/li\u003e\n\u003cli\u003eCross-species reactivity with human and rat\u003c\/li\u003e\n\u003cli\u003eTargets a key enzyme in mitochondrial and TCA cycle research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat does this antibody detect?\u003c\/strong\u003e\u003cbr\u003eHuman and rat ACO2 (mitochondrial aconitase), the TCA-cycle enzyme that converts citrate to isocitrate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhich applications is it suitable for?\u003c\/strong\u003e\u003cbr\u003eWestern blot and immunohistochemistry on paraffin-embedded sections.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs a monoclonal version available?\u003c\/strong\u003e\u003cbr\u003eYes. Catalog #L2001206 is a mouse monoclonal IgG1 anti-ACO2 antibody reactive with human ACO2 for Western blot.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat dilution should I use?\u003c\/strong\u003e\u003cbr\u003eOptimal dilutions depend on your sample and detection system. Titrate for each application and request the datasheet for starting points.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store it?\u003c\/strong\u003e\u003cbr\u003eAt −20°C in aliquots, avoiding repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSpiegel R, Pines O, Ta-Shma A, Burak E, Shaag A, Halvardson J, Edvardson S, Mahajna M, Zenvirt S, Saada A, Shalev S, Feuk L, Elpeleg O. Infantile cerebellar-retinal degeneration associated with a mutation in mitochondrial aconitase, ACO2. \u003cem\u003eAm J Hum Genet.\u003c\/em\u003e 2012;90(3):518-523.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ajhg.2012.01.009\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMetodiev MD, Gerber S, Hubert L, Delahodde A, Chretien D, Gérard X, Amati-Bonneau P, Giacomotto MC, Boddaert N, Kaminska A, Desguerre I, Amiel J, Rio M, Kaplan J, Munnich A, Rötig A, Rozet JM, Besmond C. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. \u003cem\u003eJ Med Genet.\u003c\/em\u003e 2014;51(12):834-838.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25351951\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805452955946,"sku":"BTS-L2001509","price":825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/L2001509.png?v=1790798135","url":"https:\/\/bluetigerscientific.com\/products\/aco2-antibody-center-rabbit-polyclonal","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}