{"product_id":"4-percent-magnetic-large-agarose-bead","title":"4% Magnetic Large Agarose Bead","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003e4% Magnetic Large Agarose Bead – Catalog #B2025438\u003c\/h2\u003e\n\u003cp\u003e4% Magnetic Large Agarose Beads (Catalog #B2025438) are the large-particle version of standard 4% magnetic agarose beads, composed of 4% agarose cross-linked with embedded magnetite (Fe₃O₄) particles. The larger diameter improves magnetic response and settling speed, reducing incubation time and minimizing bead carryover during pipetting. Supplied as 25 mL of resin suspension, these beads are ideal for high-throughput protein purification, affinity isolation of antibodies, and large-scale immunoprecipitation.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025438\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification, protein separation, antibody isolation, immunoprecipitation, high-throughput magnetic separation, nucleic acid extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4% magnetic agarose beads, large magnetic beads, magnetic agarose particles, affinity purification, protein isolation, antibody purification, magnetic separation beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eAgarose is a biopolymer from red seaweed with a neutral, inert surface and well-defined porosity ideal for biochemical separations. Magnetic agarose beads embed iron oxide particles within the gel matrix, allowing rapid isolation using an external magnetic field. The larger bead size in this product provides several advantages: (1) faster magnetic sedimentation due to greater mass and magnetic moment, (2) reduced bead loss during transfer with pipettes due to easier visibility and settling, and (3) improved handling in automated or high-throughput workflows. The 4% agarose concentration balances mechanical rigidity with sufficient porosity for large protein binding.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge-scale affinity chromatography for protein and antibody purification\u003c\/li\u003e\n\u003cli\u003eHigh-throughput immunoprecipitation (IP) and co-immunoprecipitation (co-IP)\u003c\/li\u003e\n\u003cli\u003eRapid magnetic separation with minimal incubation time\u003c\/li\u003e\n\u003cli\u003eBulk nucleic acid or biomolecule extraction from cell suspensions\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Gently invert the tube several times to resuspend beads before each use (do not vortex). The larger bead size makes them more robust; use standard magnetic separation racks and allow 0.5–2 minutes for full bead settling.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead coupling:\u003c\/strong\u003e Standard agarose activation methods (cyanogen bromide, epoxide) work well. The larger beads may offer reduced bead-to-beads surface contact, potentially improving ligand accessibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Insert the tube fully into a magnetic rack. The larger bead size typically allows faster settling and more complete bead capture than standard beads.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Resuspend beads gently and re-magnetize to remove unbound material.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHandling:\u003c\/strong\u003e The larger bead size makes these beads easier to see and less prone to loss during transfer, ideal for small-scale or manual workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of 4% magnetic agarose beads (large size), supplied as resin suspension\u003c\/li\u003e\n\u003cli\u003eEnhanced magnetic separation speed and ease of handling\u003c\/li\u003e\n\u003cli\u003eInert agarose matrix compatible with diverse ligand coupling chemistry\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eFaster magnetic settling reduces assay turnaround time\u003c\/li\u003e\n\u003cli\u003eLarger size minimizes bead loss and improves visibility in manual workflows\u003c\/li\u003e\n\u003cli\u003eRobust agarose matrix withstands repeated use and regeneration\u003c\/li\u003e\n\u003cli\u003eModerate porosity binds large proteins and antibody complexes effectively\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow is the large bead different from standard beads?\u003c\/strong\u003e\u003cbr\u003eLarger diameter provides faster magnetic response, quicker settling, and easier visibility. Use the same magnetic racks and similar separation protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the recommended magnetic field strength?\u003c\/strong\u003e\u003cbr\u003eStandard laboratory magnetic separation racks suitable for agarose beads work well; follow the rack manufacturer's guidelines for incubation time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads with automated purification systems?\u003c\/strong\u003e\u003cbr\u003eYes, if your system is configured for larger bead sizes. Check your equipment specifications or contact our technical team.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I couple antibodies to these beads?\u003c\/strong\u003e\u003cbr\u003eUse standard agarose activation methods (cyanogen bromide, epoxide, aldehyde). Request a methods guide or consult published protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan these beads be regenerated and reused?\u003c\/strong\u003e\u003cbr\u003eYes, if ligands are covalently attached. Regenerate with appropriate buffers and monitor performance over multiple cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBrechmann NA, Eriksson PO, Eriksson K, Oscarsson S, Buijs J, Shokri A, Hjälm G, Chotteau V. Pilot-scale process for magnetic bead purification of antibodies directly from non-clarified CHO cell culture. \u003cem\u003eBiotechnol Prog.\u003c\/em\u003e 2019;35(3):e2775.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/btpr.2775\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eChen-Cleland TA, Boffa LC, Carpaneto EM, Mariani MR, Valentin E, Mendez E, Allfrey VG. Recovery of transcriptionally active chromatin restriction fragments by binding to organomercurial-agarose magnetic beads. A rapid and sensitive method for monitoring changes in higher order chromatin structure during gene activation and repression. \u003cem\u003eJ Biol Chem.\u003c\/em\u003e 1993;268(31):23409-16.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/8226865\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLee YC, Srajer Gajdosik M, Josic D, Lin SH. Plasma membrane isolation using immobilized concanavalin A magnetic beads. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2012;909:29-41.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-61779-959-4_3\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805760024874,"sku":"BTS-B2025438","price":1235.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025438.png?v=1790801844","url":"https:\/\/bluetigerscientific.com\/products\/4-percent-magnetic-large-agarose-bead","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}