{"product_id":"4-percent-magnetic-agarose-bead","title":"4% Magnetic Agarose Bead","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003e4% Magnetic Agarose Bead – Catalog #B2025436\u003c\/h2\u003e\n\u003cp\u003e4% Magnetic Agarose Beads (Catalog #B2025436) are composite particles combining agarose—a neutral, inert polysaccharide gel matrix from red seaweed—with embedded magnetic iron oxide particles (magnetite, Fe₃O₄). The 4% agarose concentration provides moderate mechanical strength and porosity suitable for binding and elution of biomolecules. Supplied as 25 mL of resin suspension, these T1-type beads are designed for rapid magnetic separation in molecular biology and biochemistry laboratories.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025436\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead type:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eT1 Magnetic Agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification, protein separation, antibody isolation, immunoprecipitation, magnetic separation, nucleic acid extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4% magnetic agarose beads, T1 magnetic agarose, magnetic agarose particles, magnetic beads, agarose beads, magnetic separation, affinity purification beads, protein purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eAgarose is a biopolymer extracted from red algae, composed of alternating agarose and agaropectin units. Its neutral charge, inert surface, and well-defined pore structure make it ideal for gel electrophoresis, chromatography, and as a bead matrix for affinity purification. Embedding magnetic iron oxide (magnetite, Fe₃O₄) particles within agarose beads enables rapid isolation under an external magnetic field, eliminating the need for centrifugation or filtration steps in protein separation workflows. The 4% concentration balances mechanical strength with sufficient porosity for biomolecule binding. T1 beads refer to a standard size and magnetization suitable for use with common laboratory magnetic separation racks.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAffinity chromatography for protein or antibody purification\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation (IP) and co-immunoprecipitation (co-IP)\u003c\/li\u003e\n\u003cli\u003eRapid magnetic separation of conjugated or captured biomolecules\u003c\/li\u003e\n\u003cli\u003eNucleic acid extraction from cell lysates or samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Before use, gently resuspend the beads by inverting the tube several times (do not vortex vigorously, which may cause bead aggregation or damage). Use a magnetic separation rack compatible with the bead size (typically a standard lab rack for T1 beads).\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead activation:\u003c\/strong\u003e If coupling ligands (antibodies, proteins, or binding molecules), activate beads according to the ligand conjugation protocol appropriate for agarose (e.g., cyanogen bromide, epoxide, or aldehyde activation).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeparation:\u003c\/strong\u003e After incubating beads with sample, place the tube in a magnetic rack and allow 1–3 minutes for beads to settle. Carefully aspirate the supernatant while beads are held against the magnet.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Gently resuspend beads in buffer and re-magnetize multiple times to remove unbound material.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of 4% magnetic agarose beads (T1 type), supplied as resin suspension\u003c\/li\u003e\n\u003cli\u003eEfficient magnetic separation for affinity purification workflows\u003c\/li\u003e\n\u003cli\u003eInert, biocompatible agarose matrix suitable for diverse applications\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eInert agarose matrix minimizes non-specific binding\u003c\/li\u003e\n\u003cli\u003eRapid magnetic separation replaces time-consuming centrifugation\u003c\/li\u003e\n\u003cli\u003eCompatible with standard magnetic separation racks\u003c\/li\u003e\n\u003cli\u003eModerate porosity suitable for proteins, nucleic acids, and small biomolecule complexes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads without pre-coupling a ligand?\u003c\/strong\u003e\u003cbr\u003eYes, if your agarose supplier has pre-coupled antibodies, proteins, or binding molecules to the beads. Check the product specification for pre-coupled variants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I couple an antibody or protein to these beads?\u003c\/strong\u003e\u003cbr\u003eAgarose beads can be activated using cyanogen bromide, epoxide, aldehyde, or other standard chemistry. Consult a biochemistry methods manual or request guidance from our technical team.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy are the beads aggregating?\u003c\/strong\u003e\u003cbr\u003eVigorous vortexing or sonication may cause aggregation. Resuspend gently by hand inversion only.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long does magnetic separation take?\u003c\/strong\u003e\u003cbr\u003eTypically 1–3 minutes depending on bead size and tube geometry. Ensure the tube is fully inserted into the magnetic rack.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eIf covalently coupled with ligands, yes—beads can be regenerated with appropriate buffers and reused until performance declines.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLevison PR, Badger SE, Dennis J, Hathi P, Davies MJ, Bruce IJ, Schimkat D. Recent developments of magnetic beads for use in nucleic acid purification. \u003cem\u003eJ Chromatogr A.\u003c\/em\u003e 1998;816(1):107-11.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0021-9673(98)00064-8\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLee YC, Srajer Gajdosik M, Josic D, Lin SH. Plasma membrane isolation using immobilized concanavalin A magnetic beads. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2012;909:29-41.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-61779-959-4_3\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eTsunehiro M, Meki Y, Matsuoka K, Kinoshita-Kikuta E, Kinoshita E, Koike T. A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules. \u003cem\u003eJ Chromatogr B Analyt Technol Biomed Life Sci.\u003c\/em\u003e 2013;925:86-94.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jchromb.2013.02.039\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805759500586,"sku":"BTS-B2025436","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025436.png?v=1790801836","url":"https:\/\/bluetigerscientific.com\/products\/4-percent-magnetic-agarose-bead","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}