{"product_id":"25-oh-vitamin-d3-hrp-conjugated","title":"25-OH Vitamin D3, HRP Conjugated","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003e25-OH Vitamin D3, HRP Conjugated – Catalog #B2026710\u003c\/h2\u003e\n\u003cp\u003e25-OH Vitamin D3, HRP Conjugated (Catalog #B2026710) combines 25-hydroxyvitamin D3 (calcifediol), the primary circulating form of vitamin D produced by hepatic 25-hydroxylation of vitamin D3, with horseradish peroxidase (HRP). Supplied as 100 μg in solution, this conjugate is used in enzyme immunoassays and chemiluminescence assays to measure serum 25-hydroxyvitamin D concentration, the standard biomarker of vitamin D status.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026710\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e100 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eConjugate:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHorseradish peroxidase (HRP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSubstrate:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCompatible with HRP substrates (TMB, OPD, ABTS, chemiluminescent substrates)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEnzyme immunoassay (EIA), ELISA, chemiluminescence immunoassay, vitamin D quantification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25-hydroxyvitamin D3, 25-OH D3, 25-hydroxycholecalciferol, calcifediol, vitamin D metabolite, HRP conjugate, enzyme immunoassay, vitamin D assay, vitamin D detection, horseradish peroxidase conjugate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 μm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eVitamin D metabolism begins with ergocalciferol (vitamin D2) or cholecalciferol (vitamin D3) ingestion or skin synthesis. In the liver, both forms are hydroxylated by 25-hydroxylase (CYP2R1) to form 25-hydroxyvitamin D [25(OH)D or calcifediol], which circulates at the highest concentration (typically 20–100 ng\/mL). This 25-hydroxy metabolite is the best indicator of total body vitamin D stores and is the standard clinical marker of vitamin D status. Further hydroxylation by 1α-hydroxylase in the kidney produces 1,25-dihydroxyvitamin D, the most biologically active form, which regulates calcium homeostasis and immune function.\u003c\/p\u003e\n\u003cp\u003eHorseradish peroxidase is a widely used enzyme label in immunoassays, catalyzing the oxidation of chromogenic or chemiluminescent substrates to produce colored or luminescent products proportional to analyte concentration. HRP conjugation to 25-OH D3 enables sensitive detection in competitive immunoassays where the conjugate competes with sample 25-OH D3 for antibody binding.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eEnzyme immunoassay (EIA) and ELISA for 25-hydroxyvitamin D measurement\u003c\/li\u003e\n\u003cli\u003eChemiluminescence immunoassay for high-sensitivity vitamin D quantification\u003c\/li\u003e\n\u003cli\u003eDevelopment and optimization of vitamin D diagnostic assays\u003c\/li\u003e\n\u003cli\u003eResearch on vitamin D metabolism and physiological roles\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at -20°C and protect from light. Before use, allow the vial to come to room temperature and mix gently. Aliquot to avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimization:\u003c\/strong\u003e Titrate the conjugate concentration in your assay to achieve optimal signal-to-background ratio before running samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSubstrate selection:\u003c\/strong\u003e Choose HRP substrates appropriate for your detection method (colorimetric, chemiluminescent, or electrochemical).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer compatibility:\u003c\/strong\u003e Use assay buffers free of strong reducing agents that may inactivate the HRP enzyme.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e100 μg of 25-OH Vitamin D3 conjugated to horseradish peroxidase, supplied in solution\u003c\/li\u003e\n\u003cli\u003eA ready-to-use enzyme immunoassay tracer for vitamin D quantification\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSpecific for 25-hydroxyvitamin D, the clinically relevant vitamin D biomarker\u003c\/li\u003e\n\u003cli\u003eHRP enzyme label enables sensitive detection by multiple methods\u003c\/li\u003e\n\u003cli\u003eSuitable for developing competitive immunoassays\u003c\/li\u003e\n\u003cli\u003eWell-characterized substrate chemistry with established kinetics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat does this conjugate measure?\u003c\/strong\u003e\u003cbr\u003eIt is used to quantify 25-hydroxyvitamin D, the primary circulating form of vitamin D and the standard biomarker for vitamin D status.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat HRP substrates can I use?\u003c\/strong\u003e\u003cbr\u003eAny HRP substrate, including TMB (3,3',5,5'-tetramethylbenzidine), OPD (o-phenylenediamine), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and chemiluminescent substrates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs this suitable for clinical diagnostics?\u003c\/strong\u003e\u003cbr\u003eThis product is for research use only (RUO). Clinical assays require validation and regulatory approval; consult available documentation on the lot.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I optimize my assay?\u003c\/strong\u003e\u003cbr\u003eTitrate the conjugate concentration and antibody concentration to maximize signal-to-background ratio using positive and negative controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHan S, Qiu W, Zhang J, Bai Z, Tong X. Development of a Chemiluminescence Immunoassay for Quantification of 25-Hydroxyvitamin D in Human Serum. \u003cem\u003eJ Anal Methods Chem.\u003c\/em\u003e 2020;2020:9039270.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1155\/2020\/9039270\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eShrivastav TG. Preparation of horseradish peroxidase hydrazide and its use in immunoassay. \u003cem\u003eJ Immunoassay Immunochem.\u003c\/em\u003e 2003;24(3):301-9.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1081\/IAS-120022939\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLin AV. Direct ELISA. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2015;1318:61-7.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-2742-5_6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52805756780842,"sku":"BTS-B2026710","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026710.png?v=1790801827","url":"https:\/\/bluetigerscientific.com\/products\/25-oh-vitamin-d3-hrp-conjugated","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}